Daily stress protein (hsp70) cycle in chitons (Acanthopleura granulata Gmelin, 1791) which inhabit the rocky intertidal shoreline in a tropical ecosystem

Schill, Ralph O.; Gayle, Peter M. H.; Köhler, Heinz-Rüdiger

doi:10.1016/s1532-0456(02)00012-1

Cited by 20 publications

(16 citation statements)

References 15 publications

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“…There was also no evidence of greater oxidative damage of proteins, in contrast to previous work that has shown that elevated temperatures increased levels of carbonlyated proteins in limpets (Han et al, 2013;Zhang et al, 2014). Intertidal animals tailor their cellular stress response mechanisms to be quickly initiated following the onset of high tide such that recovery is complete before the start of the next low tide period (Hofmann and Somero, 1996;Tomanek and Somero, 2000;Schill et al, 2002;Clark et al, 2008;Zhang et al, 2014). Transcriptomics of Mytilus californianus have also shown upregulated gene expression of recovery mechanisms, including antioxidants, to return to cellular homeostasis during the high tide period before the next low tide period (Gracey et al, 2008;Place et al, 2012).…”

Section: Discussioncontrasting

confidence: 55%

The role of stochastic thermal environments in modulating the thermal physiology of an intertidal limpet, Lottia digitalis

Drake

Miller

Todgham

2017

Journal of Experimental Biology

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Much of our understanding of the thermal physiology of intertidal organisms comes from experiments with animals acclimated under constant conditions and exposed to a single heat stress. In nature, however, the thermal environment is more complex. Aerial exposure and the unpredictable nature of thermal stress during low tides may be critical factors in defining the thermal physiology of intertidal organisms. In the fingered limpet, Lottia digitalis, we investigated whether upper temperature tolerance and thermal sensitivity were influenced by the pattern of fluctuation with which thermal stress was applied. Specifically, we examined whether there was a differential response (measured as cardiac performance) to repeated heat stress of a constant and predictable magnitude compared with heat stress applied in a stochastic and unpredictable nature. We also investigated differences in cellular metabolism and damage following immersion for insights into biochemical mechanisms of tolerance. Upper temperature tolerance increased with aerial exposure, but no significant differences were found between predictable treatments of varying magnitudes (13°C versus 24°C versus 32°C). Significant differences in thermal tolerance were found between unpredictable trials with different heating patterns. There were no significant differences among treatments in basal citrate synthase activity, glycogen content, oxidative stress or antioxidants. Our results suggest that aerial exposure and recent thermal history, paired with relief from high low-tide temperatures, are important factors modulating the capacity of limpets to deal with thermal stress.

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Section: Discussioncontrasting

confidence: 55%

The role of stochastic thermal environments in modulating the thermal physiology of an intertidal limpet, Lottia digitalis

Drake

Miller

Todgham

2017

Journal of Experimental Biology

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“…In studies of the intertidal limpet Lottia gigantea, 70 kDa expression was induced by temperature changes over the course of hours (Miller et al, 2009) and peaked near the lower level of lethal temperatures. Similarly, Schill et al (2002) showed that the Hsp70 response in tropical intertidal chitons cycled with air temperature and was delayed by a few hours. In subtidal scallops, the temporal response of inducible Hsp70 and Hsp40 levels with temperature varied between species with the estuarine species (Argopecten irradians) up-regulating within 3 h (Brun et al, 2008).…”

Section: Discussionmentioning

confidence: 93%

Physiological consequences of the supralittoral fringe: microhabitat temperature profiles and stress protein levels in the tropical periwinkle Cenchritis muricatus (Linneaus, 1758)

2011

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Habitat selection by marine snails is profoundly affected by variations in biotic and abiotic factors. In the supralittoral fringe of Caribbean rocky shores, the littorinid Cenchritis muricatus endures a near-terrestrial existence through a combination of active microhabitat choice and, during dry periods, repose. In this study, we sought to compare knobby periwinkle body size, thermal load, water loss, and stress protein expression among common supralittoral microhabitats to determine the physiological consequences of habitat selection. In this study, we show: (1) body temperatures in these snails exhibit daily fluctuations of more than 20°C and regularly exceed 46°C, (2) microhabitats differ in thermal stress over small spatial scales, with snails on black rocks and within crevices experiencing more extreme temperatures than snails on white rocks or grass, (3) water losses of 8.5% of total snail mass do not affect survival during 1 week, and (4) Hsp70, but not Hsp90, expression varies slightly among microhabitats but at a level much lower than physiologically possible. During arousal following hydration, snails exhibited substantially higher levels of Hsp70s than snails on dry substrates in the field. When inactive, Cenchritis appears to utilize a distinctly different physiological state consistent with aestivation metabolism and does not exhibit significant up-regulation of inducible heat shock proteins (Hsps). In summary, studies lacking detailed thermal and hydration history, and relying only upon Hsp levels, may misrepresent the true physiological consequences of microhabitat choice for high-shore tropical gastropods.

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“…The ''optical volume'' (average grey scale value Â band area) of the Hsp70 bands in the immunoblots were quantified by a densitometric image analysis (Herolab EASY, Heidelberg, Germany). For this system, a standard curve relating the different weights of supernatant Hsp70 and signal intensity was established by Schill et al (2002). Each gel contained a pre-stained protein ladder (Benchmark, GibcoGRL, Life Technologies, Gaithersburg, MD) and a protein standard to minimize methodical variability.…”

Section: Stress Protein Analysismentioning

confidence: 99%

Quiescent gemmules of the freshwater sponge, Spongilla lacustris (Linnaeus, 1759), contain remarkably high levels of Hsp70 stress protein and hsp70 stress gene mRNA

et al. 2006

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With the onset of changing environmental conditions in autumn, the freshwater sponge Spongilla lacustris (Linnaeus, 1759) produces resistant stages (gemmules) which are in the state of quiescence. Gemmules stored at 8 degrees C remained quiescent; however, germination occurred within 24 hr after the water temperature was increased to 22.5 degrees C. Sponges hatched through the micropyle and subsequently built new spicules and a new canal system. The molecular and biochemical mechanisms which enable the gemmules to survive long periods of adverse conditions are not known. For the first time we focused on the role of the stress protein Hsp70 and the expression of hsp70 mRNA in S. lacustris during the development of gemmules in the state of quiescence into growing sponges. The partial sequence of hsp70 seems to be a true hsp gene since transcription could be clearly enhanced by temperature elevation. The results showed a large pool of cellular Hsp70 and hsp70 mRNA in gemmules during the state of quiescence. Within hours after a temperature trigger, the Hsp70 level decreased slowly and reached approximately the level of an adult sponge. Hsp70 presumably allows gemmules to stabilize their proteins and membranes during dormancy from autumn to spring when water temperatures change.

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Daily stress protein (hsp70) cycle in chitons (Acanthopleura granulata Gmelin, 1791) which inhabit the rocky intertidal shoreline in a tropical ecosystem

Cited by 20 publications

References 15 publications

The role of stochastic thermal environments in modulating the thermal physiology of an intertidal limpet, Lottia digitalis

The role of stochastic thermal environments in modulating the thermal physiology of an intertidal limpet, Lottia digitalis

Physiological consequences of the supralittoral fringe: microhabitat temperature profiles and stress protein levels in the tropical periwinkle Cenchritis muricatus (Linneaus, 1758)

Quiescent gemmules of the freshwater sponge, Spongilla lacustris (Linnaeus, 1759), contain remarkably high levels of Hsp70 stress protein and hsp70 stress gene mRNA

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