Daily rhythms of insulin and glucose levels in the plasma of sea bass Dicentrarchus labrax after experimental feeding

Gutiérrez, Joaquím; Carrillo, Manuel; Zanuy, Silvia; Planas, José Giner

doi:10.1016/0016-6480(84)90009-1

Cited by 126 publications

(58 citation statements)

References 23 publications

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“…Such a decrease in metabolites is most likely linked to the moment of feeding and the concomitant release of hormones. Although the fish in our study were fasted for 3 days, a feeding-entrained hormonal release could still have been present (Gutierrez et al, 1984).…”

Section: Discussionmentioning

confidence: 94%

Beta-adrenergic control of plasma glucose and free fatty acid levels in the air-breathing African catfishClarias gariepinusBurchell 1822

Heeswijk

Vianen

Thillart

et al. 2005

Journal of Experimental Biology

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In several water-breathing fish species, β-adrenergic receptor stimulation by noradrenaline leads to a decrease in plasma free fatty acid (FFA) levels, as opposed to an increase in air-breathing mammals. We hypothesised that this change in adrenergic control is related to the mode of breathing. Therefore, cannulated air-breathing African catfish were infused for 90·min with noradrenaline or with the nonselective β-agonist, isoprenaline. To identify the receptor type involved, a bolus of either a selective β 1 -antagonist (atenolol) or a selective β 2 -antagonist (ICI 118,551) was injected 15·min prior to the isoprenaline infusion. Both noradrenaline and isoprenaline led to an expected rise in glucose concentration. Isoprenaline combined with both the β 1 -and β 2 -antagonist led to higher glucose concentrations than isoprenaline alone. This could indicate the presence of a stimulatory β-adrenoceptor different from β 1 and β 2 -adrenoceptors; these two receptors thus seemed to mediate a reduction in plasma glucose concentration. Both noradrenaline and isoprenaline led to a significant decrease in FFA concentration. Whereas the β 1 -antagonist had no effect, the β 2 -antagonist reduced the decrease in FFA concentration, indicating the involvement of β 2 -adrenoceptors. It is concluded that the air-breathing African catfish reflects water-breathing fish in the adrenergic control of plasma FFA and glucose levels.

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Section: Discussionmentioning

confidence: 94%

Beta-adrenergic control of plasma glucose and free fatty acid levels in the air-breathing African catfishClarias gariepinusBurchell 1822

Heeswijk

Vianen

Thillart

et al. 2005

Journal of Experimental Biology

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“…14) sont plus faibles que celles rapportées dans la littérature : par exemple, 1 à 4,5 nglml chez la truite arc-en-ciel (Wagner et McKeown, 1982), 4 à 6 nglml chez le saumon (Plisetskaya et al, 1986), jusqu'à 10-12 nglml chez le bar (Gutierrez et al, 1987 Cependant, en dehors de la pé-riode de reproduction, le taux d'insuline plasmatique apparaît relativement stable chez les carnivores alors que des écarts mensuels importants sont notés chez les omnivores surtout chez le chevesne. Ces différences intra et interspécifiques pourraient être induites par la méthode de capture des animaux (Delahunty et al, 1978 ;Carrillo et al, 1982 ;Gutierrez et al, 1984). Si d'aprés Wagner et McKeown (1982) ou Gapp (1987), les catécholamines libérées à la suite d'un stress inhibent la sécrétion de l'insuline, selon Tilzey et al (1985a,b), leur action sur la sécrétion hormonale serait double : en fonction des taux circulants, il y aurait inhibition ou stimulation, ce qui pourrait expliquer, chez les espèces étudiées, non seulement les divergences observées dans l'évolution de I'insulinémie mais aussi, pour une part, les faibles valeurs obtenues.…”

Section: Insulinémieunclassified

Recherches sur l'état physiologique des poissons de la retenue de Pareloup : données relatives à cinq années d'étude

et al. 1994

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Résumé. -L'état physiologique des poissons a été estimé, pour une part, par l'évaluation mensuelle, au cours de quatre années (1984, 1989, 1990, 1991), de caractéristiques morphophysiologiques et de la composition somatique chez deux espèces différant par leur régime alimentaire : la perche et le gardon. Les valeurs obtenues ont démontré qu'il y a certaines années (1984, 1989), en été, un ralentissement de la croissance pondérale, une diminution ou un arrêt de l'accrétion protéique accompagné d'une modification de la concentration d'acides aminés ainsi qu'une chute de la teneur en lipides somatiques. Ces résultats indiquent nettement l'existence, dans la retenue de Pareloup, de conditions nutritionnelles plus ou moins favorables aux populations pisciaires, durant la période estivale. Par ailleurs, la détermination de paramètres sanguins, au cours d'un cycle annuel (1992)(1993), chez plusieurs espèces de poissons (brème, chevesne, brochet, sandre, perche) a révélé que si la valeur de l'hématocrite, la cholestérolémie, la triglycéridémie et la protéinémie sont, d'une manière générale, relativement stables, la glycémie varierait avec la photopériode et le taux d'insuline circulante serait en relation avec le cycle sexuel, pour la plupart des espèces.Mots-clés. -Poissons, retenue, composition somatique, paramètres sanguins.Abstract. -The physiological state of fish was first assessed by monthly evaluation in four years (1 984, 1989, 1990, 1991) of certain morphophysiological characteristics and of somatic composition in two species (perch and roach) with different dietary habits. In 1984 and 1989, a slowing down in weight gain, a decrease or an interruption in protein accretion associated with changes in amino acid contents, and a fall in somatic lipid levels were observed in summer. These results clearly point to dietary conditions 258 F. Vellas et al.in Pareloup Reservoir which are more or less favorable to fish populations during this period. In addition, blood parameter rneasurernents, carried out frorn 1992 to 1993 in various fish species (bream, chub, pike, pike-perch, perch), generally showed relatively unchanged values for hematocrit and plasma cholesterol, triglyceride and protein contents, while plasma glucose levels appeared dependent on the photoperiod and circulating insulin rates on the reproductive cycle, for most species.

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“…Plasma glucose concentration was determined by the glucose oxidase colorimetric method (GLUCOFIX; Menarini Diagnostics, Firenze, Italy) (15,29), and plasma FFAs were analyzed with the use of a commercial enzymatic method (NEFA-C, Wako Test). We measured plasma insulin levels by radioimmunoassay using bonito insulin as standard and a rabbit anti-bonito insulin as antiserum (11).…”

Section: Methodsmentioning

confidence: 99%

Nutritional and hormonal control of lipolysis in isolated gilthead seabream (Sparus aurata) adipocytes

Albalat¹,

Gómez‐Requeni²,

Rojas³

et al. 2005

American Journal of Physiology-Regulatory, Integrative and Comp

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-We examined the effects of diet composition and fasting on lipolysis of freshly isolated adipocytes from gilthead seabream (Sparus aurata). We also analyzed the effects of insulin, glucagon, and growth hormone (GH) in adipocytes isolated from fish fed with different diets. Basal lipolysis, measured as glycerol release, increased proportionally with cell concentration and time of incubation, which validates the suitability of these cell preparations for the study of hormonal regulation of this metabolic process. Gilthead seabream were fed two different diets, FM (100% of fish meal) and PP (100% of plant protein supplied by plant sources) for 6 wk. After this period, each diet group was divided into two groups: fed and fasted (for 11 days). Lipolysis was significantly higher in adipocytes from PP-fed fish than in adipocytes from FM-fed fish. Fasting provoked a significant increase in the lipolytic rate, about threefold in isolated adipocytes regardless of nutritional history. Hormone effects were similar in the different groups: glucagon increased the lipolytic rate, whereas insulin had almost no effect. GH was clearly lipolytic, although the relative increase in glycerol over control was lower in isolated adipocytes from fasted fish compared with fed fish. Together, we demonstrate for the first time that lipolysis, measured in isolated seabream adipocytes, is affected by the nutritional state of the fish. Furthermore, our data suggest that glucagon and especially GH play a major role in the control of adipocyte lipolysis.fish; nutritional and hormonal regulation; insulin; glucagon; growth hormone; fasting ADIPOSE TISSUE PLAYS A CENTRAL role in energy homeostasis in storing lipids in the form of triacylglycerols and in mobilizing them via breakdown into free fatty acids (FFAs) and glycerol (34). Adipose tissue is one of the most important lipid stores in several teleosts, although in some species liver or muscle also constitutes lipid storage organs (33). In salmonids, adipose tissue is distributed primarily in the abdominal cavity, associated with the mesenteric and pyloric ceca (31). In gilthead seabream, adipose tissue is also located periviscerally. It is known that fish adiposity changes seasonally and is affected by trophic status. High-fat feeds can lead to increases in visceral fat (4), resulting in reduced product yield and quality of cultured fish (7). In gilthead seabream, replacing fish meal with plant protein seems to alter lipid metabolism and results in smaller fat depots (10).Endocrine control of adipose tissue mobilization and storage remains almost unexplored in fish, although insulin and glucagon are clearly involved (12,24,28). Key hepatic enzymes in lipid metabolism such as hepatic lipase and acetyl-CoA carboxylase are also regulated by pancreatic hormones in vitro in isolated hepatocytes or in vivo in injected fish (13,21). Several in vivo studies suggest that growth hormone (GH) and somatolactin act together, in a complementary way, to regulate fat stores in gilthead seabream (4, 20). Howev...

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Daily rhythms of insulin and glucose levels in the plasma of sea bass Dicentrarchus labrax after experimental feeding

Cited by 126 publications

References 23 publications

Beta-adrenergic control of plasma glucose and free fatty acid levels in the air-breathing African catfishClarias gariepinusBurchell 1822

Beta-adrenergic control of plasma glucose and free fatty acid levels in the air-breathing African catfishClarias gariepinusBurchell 1822

Recherches sur l'état physiologique des poissons de la retenue de Pareloup : données relatives à cinq années d'étude

Nutritional and hormonal control of lipolysis in isolated gilthead seabream (Sparus aurata) adipocytes

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