Daily ingestion of fermented milk containing Lactobacillus casei DN114001 improves innate defense capacity in healthy middle-aged people

Abstract

Different lactic acid bacteria have often been administered as a dietary means to enhance immune system activity. Based on this statement, the aim of the current work was to test the effects of a Lactobacillus casei DN114001 fermented milk consumption on the immune response capacity in middle-age volunteers. Forty-five healthy volunteers, 24 women and 21 men (aged: 51-58 years), were randomized into two groups to receive three cups per day of a L. casei DN114001 (10(8)-10(10) ufc/g) fermented milk (n = 23), or placebo (n = 22), during an 8-week period. Measurements were performed before (day 0), and after the nutritional intervention (day 56). After the trial, no changes in immune cell proportions were detected, but the probiotic-treated group increased oxidative burst capacity of monocytes (probiotic group: p = 0.029; placebo group: p = 0.625), as well as NK cells tumoricidal activity (probiotic group: p = 0.023; placebo group: p = 0.125). Results showed that daily intake of fermented milk containing Lactobacillus casei DN114001 could have a positive effect in modulating the innate immune defense in healthy-middle-age people.

Examination of pH, Titratable Acidity and Antioxidant Activity in Fermented Milk

“…Lately there are a number of scientific papers [1], which prove and confirm that the consumption of fermented dairy products shows great contributions in maintaining health, which can be considered to be more important than the nutritional value of milk.…”

Beneficial Effects of a Synbiotic Supplement on Self-Perceived Gastrointestinal Well-Being and Immunoinflammatory Status of Healthy Adults

“…The sample size was chosen in order to provide enough statistical power for immune parameter changes on the basis of the data reviewed in the literature. [20][21][22][23] The exclusion criteria were the following: to be a vegetarian or to be on a diet, to suffer from any diagnosed gastrointestinal disease, chronic diseases such as diabetes and=or dyslipidemia, have had intestinal resections, or receiving chronic pharmacological treatment or acute antibiotic treatment 2 weeks prior to the intervention. Taking these criteria into account, 37 volunteers were finally included in this study who were judged to be in good health on the basis of physical examination and medical history.…”

Predictors for Reporting of Dietary Assessment Methods in Food-based Randomized Controlled Trials over a Ten-year Period

“…Tables 1 and 2 display the detail of each of the studies (by size of the study) that did report a dietary assessment method. In total 50 studies (20.6%) did not report the dietary assessment method used (Janatuinen et al, 1995;Sacks et al, 1995;Conlin et al, 2000;Fan et al, 2000;Kauwell et al, 2000;Knopp et al, 2000;Vuksan et al, 2000;Gregory et al, 2001;Lietz et al, 2001;Moore et al, 2001;Svetkey et al, 2001;Vollmer et al, 2001;Janatuinen et al, 2002;Pijls et al, 2002;Shankar et al, 2002;Vicennati et al, 2002;Allison et al, 2003, Berg et al, 2003Engstrom et al, 2003;Facchini et al, 2003;Gluck and Gebbers, 2003;Hadley et al, 2003;Hoyt et al, 2003;Khan et al, 2003;Upritchard et al, 2003;Vozzo et al, 2003;Wolfe et al, 2003;Xiao et al, 2003; Writing group of the PREMIER Collaborative Research Group, 2003;Chen et al, 2004;Maubach et al, 2004;McGuire et al, 2004;Miyashita et al, 2004;Parra et al, 2004;Sagara et al, 2004;Westerterp-Plantenga et al, 2004;Davey Smith et al, 2005;Svetkey et al, 200...…”

“…Although it was hypothesized that newer studies would utilize technology for dietary assessment more so than older ones, this was not found to be the case x 2 0.487 (p D 0.485). Writers of the publications also appeared to have assumed that the (Janatuinen et al, 1995, Sacks et al, 1995, Conlin et al, 2000, Fan et al, 2000, Vuksan et al, 2000, Kauwell et al, 2000, Knopp et al, 2000, Gregory et al, 2001, Lietz et al, 2001, Janatuinen et al, 2002, Pijls et al, 2002, Shankar et al, 2002, Vicennati et al, 2002, Vozzo et al, 2003, Khan et al, 2003, Engstrom et al, 2003, Hadley et al, 2003, Xiao et al, 2003, Allison et al, 2003, Wolfe et al, 2003, Berg et al, 2003, Facchini et al, 2003, Gluck and Gebbers, 2003, Hoyt et al, 2003, Writing group of the PREMIER Collaborative Research Group, 2003, Upritchard et al, 2003, Westerterp-Plantenga et al, 2004, Miyashita et al, 2004, Parra et al, 2004, Sagara et al, 2004, Maubach et al, 2004, Chen et al, 2004, Davey Smith et al, 2005, Derouiche et al, 2005, McGuire et al, 2004, Pieterse et al, 2005, Shankar et al, 2005, Lejeune et al, 2005, Benito et al, 2006<...>…”