Daily Cycle of bHLH-PAS Proteins, Ah Receptor and Arnt, in Multiple Tissues of Female Sprague–Dawley Rats

Richardson, Vicki M.; Santostefano, Michael J.; Birnbaum, Linda S.

doi:10.1006/bbrc.1998.9634

Cited by 55 publications

(39 citation statements)

References 40 publications

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“…Our results suggest that the AHR1 protein is more widely expressed than mRNA expression levels would suggest. This incongruence may be due to tissue-specific differences in the stability of AHR1 mRNA or protein, or differences in the AHR status among the fish sampled in the two studies including factors influencing tissue mRNA expression and protein concentration, for example, time of day (Richardson et al 1998). With respect to AHR2 protein, our results showing that AHR2 protein is widely expressed are consistent with the mRNA data of Karchner et al (1999).…”

Section: Discussionsupporting

confidence: 83%

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Merson

Franks

Karchner

et al. 2006

Comparative Biochemistry and Physiology Part C: Toxicology &

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The aryl hydrocarbon receptor (AHR) and AHR repressor (AHRR) proteins regulate gene expression in response to some halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons. The Atlantic killifish is a valuable model of the AHR signaling pathway, but antibodies are not available to fully characterize AHR and AHRR proteins. Using bacterially expressed AHRs, we developed specific and sensitive polyclonal antisera against the killifish AHR1, AHR2, and AHRR. In immunoblots, these antibodies recognized full-length killifish AHR and AHRR proteins synthesized in rabbit reticulocyte lysate, proteins expressed in mammalian cells transfected with killifish AHR and AHRR constructs, and AHR proteins in cytosol preparations from killifish tissues. Killifish AHR1 and AHR2 proteins were detected in brain, gill, kidney, heart, liver, and spleen. Antisera specifically precipitated their respective target proteins in immunoprecipitation experiments with in vitro-expressed proteins. Killifish ARNT2 co-precipitated with AHR1 and AHR2. These sensitive, specific, and versatile antibodies will be valuable to researchers investigating AHR signaling and other physiological processes involving AHR and AHRR proteins.

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Section: Discussionsupporting

confidence: 83%

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Merson

Franks

Karchner

et al. 2006

Comparative Biochemistry and Physiology Part C: Toxicology &

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show abstract

“…and thymus but not in spleen (38). However, we were not able to detect any significant fluctuations of Arnt gene expression in any of the tissues we examined, including liver.…”

Section: Discussioncontrasting

confidence: 66%

Characterization of Peripheral Circadian Clocks in Adipose Tissues

et al. 2006

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“…Both mRNA and protein levels of AhR in rat liver oscillates in a circadian manner (Richardson et al, 1998;Huang et al, 2002). It is not surprising that the level of CYP1A1 mRNA, one of the AhR-regulated genes, has a rhythm that is antiphase to the AhR.…”

mentioning

confidence: 99%

The Aryl Hydrocarbon Receptor Agonist 2,3,7,8-Tetrachlorodibenzo-p-dioxin Alters the Circadian Rhythms, Quiescence, and Expression of Clock Genes in Murine Hematopoietic Stem and Progenitor Cells

Garrett

Gasiewicz

2006

Mol Pharmacol

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2,3,7,, an aryl hydrocarbon receptor (AhR) agonist, has been identified as a potent immunohematopoietic toxicant with the ability to alter the number of Lin Ϫ Sca-1 ϩ cKit ϩ (LSK) bone marrow cells, a population enriched for murine hematopoietic stem cells. The biology of these cells is governed by circadian rhythms and TCDD has been shown to disrupt circadian rhythms of other biological endpoints. We investigated the effect of TCDD on the circadian rhythms of hematopoietic precursors. Female C57BL/6 mice were treated with a single oral dose of 10 g/kg TCDD. Five days later, bone marrow was harvested every 4 h for 24 h and stained for specific hematopoietic populations using fluorescently labeled antibodies. In addition, cells were placed into semisolid culture to measure different functionally defined populations. Activation of the AhR by TCDD elicited disruptions in the rhythms of LSK cell numbers and phenotypically defined myeloid and erythroid precursors. Simultaneous DNA and RNA staining revealed an abnormal in vivo rhythm of percentage of total number of LSK cells in G 0 phase of the cell cycle, suggesting disruption of stem cell quiescence. Finally, quantitative reverse transcription-polymerase chain reaction revealed that expression of AhR and Arnt mRNA within enriched hematopoietic precursors oscillates with a circadian period. Modest changes in the 24-h expression of mPer1 and mPer2 mRNA and increased AhR repressor mRNA after TCDD exposure suggest a direct effect on the molecular machinery responsible for these rhythms. Together, these data demonstrate that activation of the AhR by TCDD disrupts the circadian rhythms associated with murine hematopoietic precursors.

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Daily Cycle of bHLH-PAS Proteins, Ah Receptor and Arnt, in Multiple Tissues of Female Sprague–Dawley Rats

Cited by 55 publications

References 40 publications

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Characterization of Peripheral Circadian Clocks in Adipose Tissues

The Aryl Hydrocarbon Receptor Agonist 2,3,7,8-Tetrachlorodibenzo-p-dioxin Alters the Circadian Rhythms, Quiescence, and Expression of Clock Genes in Murine Hematopoietic Stem and Progenitor Cells

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