Daily administration of Sake Lees (Sake Kasu) reduced psychophysical stress-induced hyperalgesia and Fos responses in the lumbar spinal dorsal horn evoked by noxious stimulation to the hindpaw in the rats

Abstract

We tested whether Sake Lees (SL) had inhibitory effects on hyperalgesia in the hindpaw under psychophysical stress conditions. Male rats were subjected to repeated forced swim stress treatments (FST) from Day −3 to Day −1. Intraperiotoneal administration of SL which contained low concentration of ethanol (SLX) was conducted after each FST. On Day 0, formalin-evoked licking behaviors and Fos responses in the lumbar spinal cord (DH) and several areas within the rostral ventromedial medulla (RVM) were quantified as nociceptive responses. FST-induced hyperalgesia in the hindpaw was prevented by repeated SL and SLX treatments. Fos expression was significantly increased in DH and some areas within the RVM under FST, which was prevented by repeated SL or SLX. These findings indicated that daily administration of SL had the potential to alleviate stress-induced hyperalgesia.

Preclinical models of deep craniofacial nociception and temporomandibular disorder pain

“…Currently, interpretation of immobility behaviors as depression-like behaviors is controversial [ 179 , 180 ]; however, FS is the useful model for studying the basis for stress-induced hyperalgesia, since FS (10–20 min/day) could consistently increase nociceptive responses in the animals. For example, FS for 2–3 days causes hyperalgesia in the hindpaw, increased neural activities in the lumbar dorsal horn [ 181 , 182 ] and in the supra-spinal areas [ 183 , 184 ].…”

Effects of Sake lees (Sake-kasu) supplementation on the quality characteristics of fermented dry sausages

“…Saccharomyces cerevisiae generates carboxypeptidases, proteinases, and aminopeptidases (Van Den Hazel et al, 1996). Sake lees and their constituents have been reported to improve hepatic lipid accumulation (Kubo et al, 2017), reduce blood pressure (Saito et al, 1994), mitigate hyperalgesia (Shimizu et al, 2020), prevent allergic rhinitis-like symptoms (Kawamoto et al, 2011), and suppress acute alcohol-induced liver injury (Izu et al, 2006). Several studies reported reductions in the viable counts of aerobic bacteria in raw ham treated with Sake lees (Matsuoka et al, 2006) and altered texture in squid meat cured with Sake lees based on sensory tests, texturometry, and protein decomposition analyses (Shimomura et al, 1992).…”

Effects of stress contagion on anxiogenic‐ and orofacial inflammatory pain‐like behaviors with brain activation in mice

“…A brainstem pain-modulating system, also known as descending pain controls, with its output node in the rostral ventromedial medulla, regulates deep craniofacial nociception through the regulation of neural activities in the C1/C2 regions [44]; changes in neural activities in the rostral ventromedial medulla could facilitate nociception in various psychological stress models [45][46][47]. The rostral ventromedial medulla, including nucleus raphe magnus and LPGi, has descending 5-HT fibers to the C1/C2 region [13,48].…”