Dachengqi Decoction alleviates intestinal inflammation in ovalbumin-induced asthma by reducing group 2 innate lymphoid cells in a microbiota-dependent manner

Liu, Zirui; Li, Yalan; Li, Na; Wang, Yongan; Li, Qiuyi; Ge, Dongyu; Peng, Guiying; Zhou, Mengyu

doi:10.1016/j.jtcme.2023.01.003

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…Th17 and Treg cells have opposing roles, and the immune imbalance between Th17 and Treg cells may lead to dysregulation of the inflammatory response, further leading to asthma [10]. The interaction of cytokines such as interleukin (IL)-4, transforming growth factor-beta 1 (TGF-β1), tumor necrosis factoralpha (TNF-α), and IL-17 are also implicated in asthma pathogenesis [11,12]. The primary targets for alleviating asthma are immunity and inflammation; the immunological and inflammatory process is the underlying cause of asthma attacks [13,14].…”

Section: Introductionmentioning

confidence: 99%

Gut Microbiota-Derived Tryptophan Metabolites Alleviate Allergic Asthma Inflammation in Ovalbumin-Induced Mice

Wang,

He,

Dang

et al. 2024

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Asthma is a prevalent respiratory disease. The present study is designed to determine whether gut microbiota-derived tryptophan metabolites alleviate allergic asthma inflammation in ovalbumin (OVA)-induced mice and explore the effect and potential mechanism therein. Asthma model mice were constructed by OVA treatment, and kynurenine (KYN), indole-3-lactic acid (ILA), in-dole-3-carbaldehyde (I3C), and indole acetic acid (IAA) were administered by intraperitoneal injection. The percent survival, weight and asthma symptom score of mice were recorded. The total immunoglobulin E and OVA-specific (s)IgE in the serum and the inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) were detected by the corresponding ELISA kits. The composition of the gut microbiota and tryptophan-targeted metabolism in mouse feces were analyzed using 16S rRNA gene sequencing and targeted metabolomics, respectively. The four tryptophan metabolites improved the percent survival, weight and asthma symptoms of mice, and reduced the inflammatory cells in lung tissues, especially I3C. I3C and IAA significantly (p < 0.05) downregulated the levels of OVA-IgE and inflammatory cytokines. KYN was observed to help restore gut microbiota diversity. Additionally, I3C, KYN, and ILA increased the relative abundance of Anaeroplasma, Akkermansia, and Ruminococcus_1, respectively, which were connected with tryptophan metabolic pathways. IAA also enhanced capability of tryptophan metabolism by the gut microbiota, restoring tryptophan metabolism and increasing production of other tryptophan metabolites. These findings suggest that tryptophan metabolites may modulate asthma through the gut microbiota, offering potential benefits for clinical asthma management.

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