DABs are inorganic carbon pumps found throughout prokaryotic phyla

Desmarais, John J.; Flamholz, Avi I.; Blikstad, Cecilia; Dugan, Eli; Laughlin, Thomas G.; Oltrogge, Luke M.; Chen, Allen W; Wetmore, Kelly M.; Diamond, Spencer; Wang, Joy Y.; Savage, David F.

doi:10.1038/s41564-019-0520-8

Cited by 57 publications

(105 citation statements)

References 65 publications

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“…Our model proposes that the  pathway is a distinct category of CCM: namely, a transmembrane, photon-energized or photosynthetically-enhanced hydroxylation of CO 2 to HCO 3 -. Unidirectional moieties sharing some of these characteristics have been described or modeled in cyanobacteria, green algae, proteobacteria, and land plants (Volokita et al, 1984;Fridlyand et al, 1996;Kaplan and Reinhold, 1999;Tchernov et al, 2001;Wang and Spalding, 2014;Eichner et al, 2015;Griffiths et al, 2017;Desmarais et al, 2019).…”

Section: Relationship To Existing Ccm Definitionsmentioning

confidence: 99%

A general model for carbon isotopes in red-lineage phytoplankton: Interplay between unidirectional processes and fractionation by RubisCO

Wilkes

Pearson

2019

Geochimica et Cosmochimica Acta

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Section: Relationship To Existing Ccm Definitionsmentioning

confidence: 99%

A general model for carbon isotopes in red-lineage phytoplankton: Interplay between unidirectional processes and fractionation by RubisCO

Wilkes

Pearson

2019

Geochimica et Cosmochimica Acta

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“…The two substantially under-represented proteins on the SDS gel were identified as a protein annotated Aq_863 of 118 kDa with a probable function as an inorganic carbon pump (band b) [36] and an alkyl hydroperoxide reductase of 24 kDa (band e), both of which are probably contaminants.…”

Section: Purification and Identification Of Soeabc As The Sulfite Dehmentioning

confidence: 99%

Sulfite oxidation by the quinone-reducing molybdenum sulfite dehydrogenase SoeABC from the bacterium Aquifex aeolicus

Boughanemi

Infossi

Giudici‐Orticoni

et al. 2020

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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“…2 into the chloroplast (Hanson et al, 2016;Long et al, 2018;Desmarais et al, 2019). There have been recent improvements in approaches to tackle the issue of localizing these inorganic carbon pumps (Rolland et al, 2016;Uehara et al, 2016) alongside advances in understanding the role of the various carbonic anhydrases (Hu et al, 2015;DiMario et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Hybrid Cyanobacterial-Tobacco Rubisco Supports Autotrophic Growth and Procarboxysomal Aggregation

et al. 2019

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Much of the research aimed at improving photosynthesis and crop productivity attempts to overcome shortcomings of the primary CO 2-fixing enzyme Rubisco. Cyanobacteria utilize a CO 2-concentrating mechanism (CCM), which encapsulates Rubisco with poor specificity but a relatively fast catalytic rate within a carboxysome microcompartment. Alongside the active transport of bicarbonate into the cell and localization of carbonic anhydrase within the carboxysome shell with Rubisco, cyanobacteria are able to overcome the limitations of Rubisco via localization within a high-CO 2 environment. As part of ongoing efforts to engineer a b-cyanobacterial CCM into land plants, we investigated the potential for Rubisco large subunits (LSU) from the b-cyanobacterium Synechococcus elongatus (Se) to form aggregated Rubisco complexes with the carboxysome linker protein CcmM35 within tobacco (Nicotiana tabacum) chloroplasts. Transplastomic plants were produced that lacked cognate Se Rubisco small subunits (SSU) and expressed the Se LSU in place of tobacco LSU, with and without CcmM35. Plants were able to form a hybrid enzyme utilizing tobacco SSU and the Se LSU, allowing slow autotrophic growth in high CO 2. CcmM35 was able to form large Rubisco aggregates with the Se LSU, and these incorporated small amounts of native tobacco SSU. Plants lacking the Se SSU showed delayed growth, poor photosynthetic capacity, and significantly reduced Rubisco activity compared with both wild-type tobacco and lines expressing the Se SSU. These results demonstrate the ability of the Se LSU and CcmM35 to form large aggregates without the cognate Se SSU in planta, harboring active Rubisco that enables plant growth, albeit at a much slower pace than plants expressing the cognate Se SSU.

show abstract

DABs are inorganic carbon pumps found throughout prokaryotic phyla

Cited by 57 publications

References 65 publications

A general model for carbon isotopes in red-lineage phytoplankton: Interplay between unidirectional processes and fractionation by RubisCO

A general model for carbon isotopes in red-lineage phytoplankton: Interplay between unidirectional processes and fractionation by RubisCO

Sulfite oxidation by the quinone-reducing molybdenum sulfite dehydrogenase SoeABC from the bacterium Aquifex aeolicus

Hybrid Cyanobacterial-Tobacco Rubisco Supports Autotrophic Growth and Procarboxysomal Aggregation

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