DA-9801 Promotes Neurite Outgrowth &lt;i&gt;via&lt;/i&gt; ERK1/2-CREB Pathway in PC12 Cells

Won, Jong Hoon; Ahn, Kyong Hoon; Back, Moon Jung; Ha, Hae Chan; Jang, Ji Min; Kim, Ha Hyung; Choi, Sang-Zin; Son, Miwon; Kim, Dae Kyong

doi:10.1248/bpb.b14-00236

Cited by 17 publications

(10 citation statements)

References 61 publications

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“…Lin identified the involvement of cAMP/PKA and ERK dependent CREB signaling pathways in the luteolin-mediated miR-132 expression and neuritogenesis of PC12 cells [47]. Won demonstrated that DA-9801 exerts its beneficial effects of stimulating neurite outgrowth through the ERK1/2-CREB pathway in PC12 cells [48]. Behavioral analyses of animals with altered ERK signaling have revealed a central involvement of this cascade in learning and memory [49], and it has been reported that ERK activity was decreased in dentate gyrus of aged rats, which did not sustain LTP [50, 51].…”

Section: Discussionmentioning

confidence: 99%

Ketamine administered pregnant rats impair learning and memory in offspring via the CREB pathway

Guo

et al. 2017

Oncotarget

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Ketamine has been reported to impair the capacity for learning and memory. This study examined whether these capacities were also altered in the offspring and investigated the role of the CREB signaling pathway in pregnant rats, subjected to ketamine-induced anesthesia. On the 14th day of gestation (P14), female rats were anesthetized for 3 h via intravenous ketamine injection (200 mg/Kg). Morris water maze task, contextual and cued fear conditioning, and olfactory tasks were executed between the 25th to 30th day after birth (B25-30) on rat pups, and rats were sacrificed on B30. Nerve density and dendritic spine density were examined via Nissl’s and Golgi staining. Simultaneously, the contents of Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII), p-CaMKII, CaMKIV, p-CaMKIV, Extracellular Regulated Protein Kinases (ERK), p-ERK, Protein Kinase A (PKA), p-PKA, cAMP-Response Element Binding Protein (CREB), p-CREB, and Brain Derived Neurotrophic Factor (BDNF) were detected in the hippocampus. We pretreated PC12 cells with both PKA inhibitor (H89) and ERK inhibitor (SCH772984), thus detecting levels of ERK, p-ERK, PKA, p-PKA, p-CREB, and BDNF. The results revealed that ketamine impaired the learning ability and spatial as well as conditioned memory in the offspring, and significantly decreased the protein levels of ERK, p-ERK, PKA, p-PKA, p-CREB, and BDNF. We found that ERK and PKA (but not CaMKII or CaMKIV) have the ability to regulate the CREB-BDNF pathway during ketamine-induced anesthesia in pregnant rats. Furthermore, ERK and PKA are mutually compensatory for the regulation of the CREB-BDNF pathway.

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Section: Discussionmentioning

confidence: 99%

Ketamine administered pregnant rats impair learning and memory in offspring via the CREB pathway

Guo

et al. 2017

Oncotarget

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“…10-1341692-0000). Several preclinical studies have been done to evaluate the therapeutic efficacy and mechanism of DA-9801 in vitro and in vivo (Kim et al, 2011a;Ji et al, 2013;Jin et al, 2013;Lee et al, 2013;Moon et al, 2014;Song et al, 2014;Won et al, 2015). Recently, in January 2015, DA-9801 ended its US phase II clinical trial for assessing the safety and effectiveness in the treatment of subjects with diabetic neuropathy (ClinicalTrials.gov identifier NCT01822925).…”

Section: Introductionmentioning

confidence: 99%

Combined Application of UHPLC‐QTOF/MS, HPLC‐ELSD and ¹H–NMR Spectroscopy for Quality Assessment of DA‐9801, A Standardised Dioscorea Extract

Kang

Ryu

Cho

et al. 2016

Phytochemical Analysis

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“…Fisetin reduced the phosphorylation of p38 but not that of ERK1/2, JNK1/2, or AKT. 42 Moreover, Won et al 43 anticancer drugs. 44,45 Our study revealed that DNE exerted no significant cytotoxic effects on cell viability (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Dioscorea nipponica Makino suppresses TPA‐induced migration and invasion through inhibition of matrix metalloproteinase‐9 in human cervical cancer cells

Lee

Chou

Hsin

et al. 2020

Environmental Toxicology

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Dioscorea nipponica Makino has been used for the treatment of chronic bronchitis, rheumatoid arthritis, cough, and asthma. Several studies have established the antitumor effect of D. nipponica Makino extract (DNE). However, no investigations have considered the antimetastatic potential of DNE in cervical cancer cells. The present study examined the effects of DNE on cervical cancer cells treated with 12-Otetradecanoylphorbol-13-acetate and characterized the possible molecular mechanisms. MTT assay results indicated that DNE exhibited very low cytotoxicity, and DNE significantly reduced the invasion and migration abilities of cervical cancer cells. Gelatin zymography analysis revealed that DNE significantly inhibited matrix metalloproteinase-9 (MMP-9) activity. Reverse transcription-polymerase chain reaction assay results revealed that DNE treatment inhibited the MMP-9 mRNA levels of HeLa and SiHa cells. Western blot results revealed that DNE significantly diminished the ERK1/2 phosphorylation. In conclusion, we revealed that the antimetastatic effects of DNE on cervical cancer cells are due to its inhibition of MMP-9 expression through the ERK1/2 pathway.

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DA-9801 Promotes Neurite Outgrowth <i>via</i> ERK1/2-CREB Pathway in PC12 Cells

Cited by 17 publications

References 61 publications

Ketamine administered pregnant rats impair learning and memory in offspring via the CREB pathway

Ketamine administered pregnant rats impair learning and memory in offspring via the CREB pathway

Combined Application of UHPLC‐QTOF/MS, HPLC‐ELSD and ¹H–NMR Spectroscopy for Quality Assessment of DA‐9801, A Standardised Dioscorea Extract

Dioscorea nipponica Makino suppresses TPA‐induced migration and invasion through inhibition of matrix metalloproteinase‐9 in human cervical cancer cells

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DA-9801 Promotes Neurite Outgrowth <i>via</i> ERK1/2-CREB Pathway in PC12 Cells

Cited by 17 publications

References 61 publications

Ketamine administered pregnant rats impair learning and memory in offspring via the CREB pathway

Ketamine administered pregnant rats impair learning and memory in offspring via the CREB pathway

Combined Application of UHPLC‐QTOF/MS, HPLC‐ELSD and 1H–NMR Spectroscopy for Quality Assessment of DA‐9801, A Standardised Dioscorea Extract

Dioscorea nipponica Makino suppresses TPA‐induced migration and invasion through inhibition of matrix metalloproteinase‐9 in human cervical cancer cells

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Product

Resources

About

Combined Application of UHPLC‐QTOF/MS, HPLC‐ELSD and ¹H–NMR Spectroscopy for Quality Assessment of DA‐9801, A Standardised Dioscorea Extract