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doi:10.1002/bit.v101:2

Biotech & Bioengineering

2008

DOI: 10.1002/bit.v101:2

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Abstract: The proteomic era has increased the demand for simple and reliable methods to clone and express new target genes and purify their encoded proteins. Research in this area has recently delivered two new methods that independently simplify these goals. On the cloning end, the Invitrogen corporation has developed Gateway

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Cited by 6 publications

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Filamentous bulking caused by Thiothrix species is efficiently controlled in full-scale wastewater treatment plants by implementing a sludge densification strategy

Henriet

Meunier

Henry

et al. 2017

Sci Rep

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Filamentous bulking caused by Thiothrix species is responsible for sludge washout and loss of performance in dairy wastewater treatment plants. A long-term study was conducted over 1.5 years to test three different mitigation strategies in a full-scale plant composed of two parallel sequential batch reactors (SBR1 and 2). Strategies based on polyaluminium chloride addition and volatile fatty acids reduction were ineffective to permanently solve the problem. On the contrary, modification of the reactor cycle based on the implementation of a periodic starvation proved efficient to solve the biomass wash-out and drastically reduce the sludge volume index in both reactors. Bacterial diversity analysis using 16S amplicon sequencing and quantitative PCR indicated a reduction of Thiothrix abundance from 51.9 to 1.0% in SBR1 and from 71.8 to 0.6% in SBR2. Simultaneously, the abundance of the glycogen-accumulating bacterium Candidatus Competibacter increased in both reactors. Microscopy analysis confirmed the transition between a bulking sludge towards a granular-like sludge. This study confirms the applicability of a periodic starvation to (1) solve recurring Thiothrix bulking, (2) convert loose aggregates into dense and compact granular-like structures and (3) considerably reduce energy demand for aeration.

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Filamentous bulking caused by Thiothrix species is efficiently controlled in full-scale wastewater treatment plants by implementing a sludge densification strategy

Henriet

Meunier

Henry

et al. 2017

Sci Rep

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An integrated approach: advances in the use ofClostridiumfor biofuel

Kök

2015

Biotechnology and Genetic Engineering Reviews

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Almost 90% of our energy comes from fossil fuels, which are both limited and polluting, hence the need to find alternative sources. Biofuels can provide a sustainable and renewable source of energy for the future. Recent significant advances in genetic engineering and fermentation technology have made microbial bio-based production of chemicals from renewable resources more viable. Clostridium species are considered as promising micro-organisms for the production of a wide range of chemicals for industrial use. However, a number of scientific challenges still need to be overcome to facilitate an economically viable production system. These include the use of cheap non-food-based substrates, a better understanding of the metabolic processes involved, improvement of strains through genetic engineering and innovation in process technology. This paper reviews recent developments in these areas, advancing the use of Clostridium within an industrial context especially for the production of biofuels.

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Influence of anoxic to aerobic volume ratio on sludge settleability and bacterial community structure in a denitrifying–nitrifying activated sludge system

Chen

Yuan

et al. 2014

Desalination and Water Treatment

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Untitled

Cited by 6 publications

References 0 publications

Filamentous bulking caused by Thiothrix species is efficiently controlled in full-scale wastewater treatment plants by implementing a sludge densification strategy

Filamentous bulking caused by Thiothrix species is efficiently controlled in full-scale wastewater treatment plants by implementing a sludge densification strategy

An integrated approach: advances in the use ofClostridiumfor biofuel

Influence of anoxic to aerobic volume ratio on sludge settleability and bacterial community structure in a denitrifying–nitrifying activated sludge system

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