D-Tryptophan governs biofilm formation rates and bacterial interaction in P. mendocina and S. aureus

Ghosh, Saheli; Qureshi, Asifa; Purohit, Hemant J.

doi:10.1007/s12038-018-9841-7

Cited by 24 publications

(21 citation statements)

References 44 publications

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“…In a sister study analysis of dissolved organic matter (DOM) from samples collected concurrently with our biofilm samples revealed that all benthic organisms in our study increased organic matter exudates in response to nutrient addition and corals exude more proteinaceous organic matter than other benthic organisms—specifically, the exudation and accumulation of tryptophan-like, tyrosine-like, and phenalalanine-like organic matter was enriched in coral treatments 17 . Tyrosine and tryptophan along with other small cyclic compounds have been shown to inhibit biofilm formation 77 by inhibiting cellular communication 78 , 79 and flagellar motility 80 . While reduction in biofilm formation and successional dynamics was not observed, it was clear from our study that coral and algal biofilms were distinct throughout the experiment and followed different successional trajectories.…”

Section: Discussionmentioning

confidence: 99%

Coral reef biofilm bacterial diversity and successional trajectories are structured by reef benthic organisms and shift under chronic nutrient enrichment

Remple

Silbiger

Quinlan

et al. 2021

npj Biofilms Microbiomes

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Work on marine biofilms has primarily focused on host-associated habitats for their roles in larval recruitment and disease dynamics; little is known about the factors regulating the composition of reef environmental biofilms. To contrast the roles of succession, benthic communities and nutrients in structuring marine biofilms, we surveyed bacteria communities in biofilms through a six-week succession in aquaria containing macroalgae, coral, or reef sand factorially crossed with three levels of continuous nutrient enrichment. Our findings demonstrate how biofilm successional trajectories diverge from temporal dynamics of the bacterioplankton and how biofilms are structured by the surrounding benthic organisms and nutrient enrichment. We identify a suite of biofilm-associated bacteria linked with the orthogonal influences of corals, algae and nutrients and distinct from the overlying water. Our results provide a comprehensive characterization of marine biofilm successional dynamics and contextualize the impact of widespread changes in reef community composition and nutrient pollution on biofilm community structure.

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Section: Discussionmentioning

confidence: 99%

Coral reef biofilm bacterial diversity and successional trajectories are structured by reef benthic organisms and shift under chronic nutrient enrichment

Remple

Silbiger

Quinlan

et al. 2021

npj Biofilms Microbiomes

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“…Crystal violet binding assay in 6ml microplates were performed as described in Ghosh et al (2017a) after every 24 hrs of interval upto 288 hrs under static and shaking conditions. For the enumeration of attached cells, standard curve with positive correlation between crystal violet retention O.D and CFU count was determined (r 2 = 0.965 at p < 0.05) as per the method described in Ghosh et al (2019a). The rate of bio lm formation were also determined according to Ghosh et al (2019a).…”

Section: Bio Lm Formation Analysis By Pseudomonas Simiae Egd-aq6 In Media Amended With 2-4-dmentioning

confidence: 99%

“…For the enumeration of attached cells, standard curve with positive correlation between crystal violet retention O.D and CFU count was determined (r 2 = 0.965 at p < 0.05) as per the method described in Ghosh et al (2019a). The rate of bio lm formation were also determined according to Ghosh et al (2019a). The suspended cultures from the microplates i.e., unattached cells (planktonic) growth rate was determined as per Wood et al (2019).…”

Section: Bio Lm Formation Analysis By Pseudomonas Simiae Egd-aq6 In Media Amended With 2-4-dmentioning

confidence: 99%

Managing Gene Expression in Pseudomonas Simiae EGD-AQ6 for Chloroaromatic Compound Degradation

Ghosh¹,

Purohit²,

Qureshi³

2021

Preprint

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Pseudomonas simiae EGD-AQ6 showed utilization of chloroaromatic compound, 2-4-dichlorophenoxyacetic acid (2,4-D) efficiently in its biofilm phenotype. The differential rates of accumulation of intermediate metabolite 4-chlorocatechol (4-CCA) were significant in both planktonic and biofilm phenotypes; also increased number of biofilm cells were observed during 2,4-D utilization. Interestingly, response surface analysis demonstrated the combined positive effects of 2,4-D degradation and 4-CCA accumulations. Also, gene expression profiles showed significant up-regulation of degradative and biofilm genes (particularly pellicle forming genes) in the biofilm phenotypes than their planktonic counterparts, thereby confirming occurrence of phenotype variations of Pseudomonas simiae EGD-AQ6 during chloroaromatic degradation. Furthermore, the sequence similarity of the 2-4-D catabolic genes and biofilm forming proteins (pel ABCDEFG and pga ABCD) which are responsible for building carbohydrate rich extracellular matrix, were significant with the respective organisms as revealed through genome analysis. This is the first report, which endorses this Pseudomonas simiae species to be unique in chloro-aromatic degradation through phenotype variation, thereby proving as a potential candidate in the improvement of bioremediation technologies.

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“…Saheli Ghosh et al (2019) Figure 14.1. Metabolic pathways for plastic degradation by biofilm forming microbes.…”

Section: Engineered Pathwaysmentioning

confidence: 99%

“…Biofilms are functionally, and phylogenetically diverse communities of bacteria, fungi, and algae, conjointly termed as a microbial conglomeration, attached to a surface (Ghosh et al, 2017b). They are mostly embedded in extracellular polymeric substance (EPS) (Ghosh et al, 2016;Ghosh et al, 2019). Biofilm provides a plethora of benefits for survival and competition strategies, which includes bioavailability of nutrients, horizontal gene transfer, cell viability and prevents toxic shock (Qureshi et al, 2015;Ghosh et al, 2017a).…”

Section: Introductionmentioning

confidence: 99%

Microbial degradation of plastics: Biofilms and degradation pathways

Ghosh¹,

Qureshi²,

Purohit³

2019

Contaminants in Agriculture and Environment: Health Risks and Remediation

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Plastics are recalcitrant polymers released in the environment through unpredicted use leading to accumulation and increased water and soil pollution. Transportation of these recalcitrant polymers in agricultural soil, sediment, and water has been causing concerns for environmentalists. Biofilm community adhered on plastic polymers have a significant contribution in their degradation as they warrant bioavailability of substrates, sharing of metabolites and increased cell viability thereby accelerating biodegradation. Metabolic enzymes of the microbes can be exploited as a potent tool for polymer degradation. However very little or Chapter contents Introduction …………………………………………………………………………………………………….. Plastic degradation pathways in bacteria …………………………………………………………………… Natural metabolic pathways ……………………………………………………………………………… Engineered pathways ……………………………………………………………………………………… Biofilm forming microbes involved in degradation ……………………………………………………….. Bacteria ………………………………………………………………………………………………………. Fungus ………………………………………………………………………………………………………. Influence of biofilm on plastic degradation ………………………………………………………………... Biofilm-plastic interactions ………………………………………………………………………………….. Conclusion ……………………………………………………………………………………………………... Acknowledgments ……………………………………………………………………………………………. References ……………………………………………………………………………………………………… 185 no reports are available about the influence of biofilm and plastic degradation and vice versa. The present chapter reports the impact of biofilm microbes in the degradation of commonly used plastics. Furthermore, potent microorganisms and their interactions with the plastic surface has been deciphered, which would serve as a better understanding of the utilization of biofilm-based methods in the development of plastic waste management.

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D-Tryptophan governs biofilm formation rates and bacterial interaction in P. mendocina and S. aureus

Cited by 24 publications

References 44 publications

Coral reef biofilm bacterial diversity and successional trajectories are structured by reef benthic organisms and shift under chronic nutrient enrichment

Coral reef biofilm bacterial diversity and successional trajectories are structured by reef benthic organisms and shift under chronic nutrient enrichment

Managing Gene Expression in Pseudomonas Simiae EGD-AQ6 for Chloroaromatic Compound Degradation

Microbial degradation of plastics: Biofilms and degradation pathways

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