“…The FRAP assay was adapted from previous studies. 65,66 The reaction mixture (2 mL total volume) consisted of EtOH solution of tested compounds (nal concentration 25, 50, 100, 200, and 300 mM), 100 mL of 0.1% solution of FeSO 4 $7H 2 O in H 2 O, 200 mL of 0.25% EtOH solution of a,a ′ -dipyridyl, and 50% aqueous EtOH to 2 mL of the total value. BHT was used as a reference compound.…”