D-Penicillamine prolongs survival and lessens copper-induced toxicity in Drosophila melanogaster

Abstract

D-penicillamine (DPA) is an amino-thiol that has been established as a copper chelating agent for the treatment of Wilson’s disease. DPA reacts with metals to form complexes and/or chelates. Here, we investigated the survival rate extension capacity and modulatory role of DPA on Cu2+-induced toxicity in Drosophila melanogaster. Adult Wild type (Harwich strain) flies were exposed to Cu2+ (1 mM) and/or DPA (50 μM) in the diet for 7 days. Additionally, flies were exposed to acute Cu2+ (10 mM) for 24 h, followed by DPA (50 μM) treatment for 4 days. Thereafter, the antioxidant status [total thiol (T-SH) and glutathione (GSH) levels and glutathione S-transferase and catalase activities] as well as hydrogen peroxide (H2O2) level and acetylcholinesterase activity were evaluated. The results showed that DPA treatment prolongs the survival rate of D. melanogaster by protecting against Cu2+-induced lethality. Further, DPA restored Cu2+-induced depletion of T-SH level compared to the control (P < 0.05). DPA also protected against Cu2+ (1 mM)-induced inhibition of catalase activity. In addition, DPA ameliorated Cu2+-induced elevation of acetylcholinesterase activity in the flies. The study may therefore have health implications in neurodegenerative diseases involving oxidative stress such as Alzheimer’s disease.

Effects of Arbuscular Mycorrhizal Fungi-Colonized Populus alba × P. berolinensis Seedlings on the Microbial and Metabolic Status of Gypsy Moth Larvae

“…Our results revealed that 25 DAMs were identified in both GM and GI groups. In the comparative analysis of GM and CK groups, it was observed that sev-eral DAMs that are critical for growth, reproduction, and detoxification metabolism were significantly reduced in the GM group, such as uridine, 5, 6-dihydroxyindole-2-carboxylic acid, and penicillamine [36][37][38]. These results indicated that the metabolic disorder of gypsy moth larvae in the GM treatment group occurred, consistent with the growth retardation of gypsy moth larvae mentioned above.…”

Providing multicolor plasmonic patterns with graphene quantum dots functionalized d‐penicillamine for visual recognition of V(V), Cu (II), and Fe(III): Colorimetric fingerprints of GQDs‐DPA for discriminating ions in human urine samples

“…The same GQD accumulation process might also occur in the presence of V 5+ and considerably enhance the fluorescence intensity of GQD. Regarding the fact that DPA has been considered as Cu 2+ chelator, 41‐43 the GQD‐DPA can be accumulated through N‐Cu 2+ ‐N and S − ‐Cu 2+ ‐S − interactions of copper ion‐DPA. Thus, in the presence of Cu 2+ , considering the chelating behavior of DPA toward Cu 2+ , an immediate and unstable color alternation occurred.…”