D/H ratios of fatty acids from marine particulate organic matter in the California Borderland Basins

Jones, Ashley A.; Sessions, Alex L.; Campbell, Brian; Li, Chao; Valentine, David L.

doi:10.1016/j.orggeochem.2007.11.001

Cited by 35 publications

(39 citation statements)

References 55 publications

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“…FA from the SB\13CO2\12CH4 and SB\13CH4 incubations were not enriched in D beyond previously reported background levels (Jones et al, 2008). Similar to the trend observed in 13C enrichments, 16:1 FA from SB\13CH4\CH3D had the largest D enrichment of any FA analyzed, while 16:0 FA displayed slight enrichment (Table 7).…”

Section: Microbial Communities and Stable Isotope Probingsupporting

confidence: 74%

“…Similar to the trend observed in 13C enrichments, 16:1 FA from SB\13CH4\CH3D had the largest D enrichment of any FA analyzed, while 16:0 FA displayed slight enrichment (Table 7). 14:0 and 18:0 fatty acids for this sample reflect background values for δD (Jones et al, 2008) The uptake of isotope labels into fatty acids indicative of methanotrophs suggests that bacteria actively and vigorously consume methane in these deep ocean environments. While the 13C-based technique used here has previously been employed to probe methanotroph DNA and lipids from terrestrial and marine sediments (Holmes et al, 1996;Hutchens et al, 2004;, DNA of methylotrophs in marine surface waters (Neufeld et al, 2008a;Neufeld et al, 2008b), and DNA of methanotrophs from the Deepwater Horizon's contaminated plumes , we are aware of no published studies using SIP to probe lipids of natural methanotroph communities active at nanomolar methane concentration in deep oceanic waters.…”

Section: Microbial Communities and Stable Isotope Probingmentioning

confidence: 73%

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Assessing the Efficacy of the Aerobic Methanotrophic Biofilter in Methane Hydrate Environments

Valentine¹

2012

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Section: Microbial Communities and Stable Isotope Probingsupporting

confidence: 74%

Section: Microbial Communities and Stable Isotope Probingmentioning

confidence: 73%

Assessing the Efficacy of the Aerobic Methanotrophic Biofilter in Methane Hydrate Environments

Valentine¹

2012

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“…The pattern is supported by previous culture studies of bacteria (19)(20)(21) and plants (11,33) and is consistent with field observations. For example, fatty acids with odd carbon-numbered chains are substantially D-enriched (by up to 100‰) relative to those with even-numbered chains in marine POM (17). The former are attributed to heterotrophic bacteria, whereas the latter are likely the products of photosynthetic algae.…”

Section: Discussionmentioning

confidence: 99%

“…Recent surveys of lipids in marine environments have hinted at even greater variability in isotopic compositions. Jones et al (17) measured fatty acids extracted from coastal marine particulate organic matter (POM) and found ␦D values ranging from Ϫ73 to Ϫ237‰. Measurements of lipids from marine sediments have extended this range from Ϫ32 to Ϫ348‰ for lipids with n-alkyl skeletons and Ϫ148 to Ϫ469‰ for those with isoprenoid skeletons (18).…”

mentioning

confidence: 99%

Large D/H variations in bacterial lipids reflect central metabolic pathways

Zhang¹,

Gillespie

Sessions

2009

Proc. Natl. Acad. Sci. U.S.A.

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195

268

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Large hydrogen-isotopic (D/H) fractionations between lipids and growth water have been observed in most organisms studied to date. These fractionations are generally attributed to isotope effects in the biosynthesis of lipids, and are frequently assumed to be approximately constant for the purpose of reconstructing climatic variables. Here, we report D/H fractionations between lipids and water in 4 cultured members of the phylum Proteobacteria, and show that they can vary by up to 500‰ in a single organism. The variation cannot be attributed to lipid biosynthesis as there is no significant change in these pathways between cultures, nor can it be attributed to changing substrate D/H ratios. More importantly, lipid/water D/H fractionations vary systematically with metabolism: chemoautotrophic growth (approximately ؊200 to ؊400‰), photoautotrophic growth (؊150 to ؊250‰), heterotrophic growth on sugars (0 to ؊150‰), and heterotrophic growth on TCA-cycle precursors and intermediates (؊50 to ؉200‰) all yield different fractionations. We hypothesize that the D/H ratios of lipids are controlled largely by those of NADPH used for biosynthesis, rather than by isotope effects within the lipid biosynthetic pathway itself. Our results suggest that different central metabolic pathways yield NADPH-and indirectly lipids-with characteristic isotopic compositions. If so, lipid ␦D values could become an important biogeochemical tool for linking lipids to energy metabolism, and would yield information that is highly complementary to that provided by 13 C about pathways of carbon fixation.fatty acids ͉ fractionation ͉ metabolism ͉ hydrogen isotopes T he hydrogen-isotopic composition ( 2 H/ 1 H or D/H ratio, commonly expressed as a ␦D value) of lipids is being explored by scientists with diverse interests, including the origins of natural products (1, 2), biogeochemical cycles (3), petroleum systems (4), and paleoclimate (5-7). Because the D/H ratios of lipids are generally conserved over Ϸ10 6 -year time scales (8), they are a potentially useful tracer of biogeochemical pathways and processes in the environment. Most research to date has focused on higher plants, in which environmental water is the sole source of external hydrogen and consequently provides primary control over the D/H ratio of biosynthesized lipids (9). Although ␦D values for plant lipids and environmental water are generally well correlated, they are also substantially offset from each other. The biochemical basis for this lipid/water fractionation is not well understood. It is generally assumed to arise from a combination of isotope effects during photosynthesis and the biosynthesis of lipids (9-12), and is often treated as approximately constant to reconstruct isotopic compositions of environmental water as a paleoclimate proxy.There is, however, mounting evidence that the net D/H fractionation between lipids and water can vary by up to 150‰ in plants, even in the same organism (12-16). Modest fractionations associated with fatty acid elongation and desaturation ...

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“…Compound-specific hydrogen isotope measurements of lipid biomarkers are an emerging tool with diverse applications to microbial community dynamics (Osburn et al, 2011;Heinzelmann et al, 2016), organic matter cycling (Jones et al, 2008;Li et al, 2009), and paleoclimatology (Sachse et al, 2012, and sources therein). The hydrogen isotopic composition of source water exerts a firstPublished by Copernicus Publications on behalf of the European Geosciences Union.…”

Section: Introductionmentioning

confidence: 99%

Interplay of community dynamics, temperature, and productivity on the hydrogen isotope signatures of lipid biomarkers

2017

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Abstract. The hydrogen isotopic composition (δ 2 H) of lipid biomarkers has diverse applications in the fields of paleoclimatology, biogeochemistry, and microbial community dynamics. Large changes in hydrogen isotope fractionation have been observed among microbes with differing core metabolisms, while environmental factors including temperature and nutrient availability can affect isotope fractionation by photoautotrophs. Much effort has gone into studying these effects under laboratory conditions with single species cultures. Moving beyond controlled environments and quantifying the natural extent of these changes in freshwater lacustrine settings and identifying their causes is essential for robust application of δ 2 H values of common short-chain fatty acids as a proxy of net community metabolism and of phytoplankton-specific biomarkers as a paleohydrologic proxy.This work targets the effect of community dynamics, temperature, and productivity on 2 H/ 1 H fractionation in lipid biomarkers through a comparative time series in two central Swiss lakes: eutrophic Lake Greifen and oligotrophic Lake Lucerne. Particulate organic matter was collected from surface waters at six time points throughout the spring and summer of 2015, and δ 2 H values of short-chain fatty acids, as well as chlorophyll-derived phytol and the diatom biomarker brassicasterol, were measured. We paired these measurements with in situ incubations conducted with NaH 13 CO 3 , which were used to calculate the production rates of individual lipids in lake surface water. As algal productivity in-

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D/H ratios of fatty acids from marine particulate organic matter in the California Borderland Basins

Cited by 35 publications

References 55 publications

Assessing the Efficacy of the Aerobic Methanotrophic Biofilter in Methane Hydrate Environments

Assessing the Efficacy of the Aerobic Methanotrophic Biofilter in Methane Hydrate Environments

Large D/H variations in bacterial lipids reflect central metabolic pathways

Interplay of community dynamics, temperature, and productivity on the hydrogen isotope signatures of lipid biomarkers

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