D-glucose transport in decapod crustacean hepatopancreas

Verri, Tiziano; Mandal, A.; Zilli, L.; Bossa, D.; Mandal, P.K.; Ingrosso, L.; Zonno, V.; Vilella, Sebastiano; Ahearn, Gregory A.; Storelli, Carlo

doi:10.1016/s1095-6433(00)80303-7

Cited by 30 publications

(52 citation statements)

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“…The carbohydrates are not essential for crustaceans; shrimp appear to be able to utilize complex carbohydrates better than simple ones such as glucose, which is quickly absorbed and released into the haemolymph, resulting in a physiologically abnormal elevation of plasma glucose levels (New, 1976(New, , 1990Shiau & Peng, 1992). Starch is nowadays the typical carbohydrate in formulated feeds for crustaceans; it is well hydrolyzed by shrimp such as Fenneropenaeus indicus and Litopenaeus vannamei but poorly hydrolyzed by lobsters (Verri et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Influence of dietary starch and cellulose levels on the metabolic profile and apparent digestibility in penaeoid shrimp

Velurtas¹,

Díaz²,

Fernández-Gimenez³

et al. 2011

lajar

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ABSTRACT. The present study compared the effect of different starch/cellulose ratios (30/0, 20/10, 10/20, 0/30) on the metabolic response and apparent digestibility in two species of penaeoids: Artemesia longinaris and Pleoticus muelleri. Adult animals were used in order to obtain sufficient quantities of haemolymph and faecal material for analysis. No significant differences were found in levels of plasma metabolites in P. muelleri, but in A. longinaris, a significant increase was observed in glucose, total protein, and cholesterol in correlation with increased dietary starch. The apparent digestibility coefficients decreased from 83.7% to 51.2% (A. longinaris) and from 71.9% to 7.6% (P. muelleri) as the dietary starch levels increased. The ratio of amylase activity to protease activity (A/P ratio) declined in A. longinaris when the percentage of dietary starch increased. In contrast, the A/P ratio for P. muelleri increased with higher starch concentrations. These results demonstrated a close relationship between the feeding habits and digestive physiology of the two species studied; they also suggest a more herbivorous behavior for A. longinaris and more omnivorous habits for P. muelleri. Keywords: Artemesia longinaris, Pleoticus muelleri, apparent digestibility, carbohydrate metabolism, cellulose, starch, haemolymph, Argentina.Influencia del nivel de almidón y celulosa en la dieta sobre el perfil metabólico y digestibilidad aparente en camarones penaeoideos RESUMEN. En el presente estudio se comparó el efecto de diferentes concentraciones de almidón/celulosa (30/0; 20/10; 10/20; 0/30) sobre la respuesta metabólica y la digestibilidad aparente en dos especies de peneidos, Artemesia longinaris y Pleoticus muelleri. Se utilizaron animales adultos a fin de obtener cantidades suficientes de hemolinfa y heces para los análisis. No hubo diferencias significativas en los niveles de metabolitos plasmáticos en P. muelleri, en cambio en A. longinaris se observó un incremento significativo de la glucosa, proteínas totales y colesterol en relación con el aumento del almidón en la dieta. Los coeficientes de digestibilidad aparente disminuyeron de 83,7% a 51,2% (A. longinaris) y de 71,9% a 7,6% (P. muelleri) a medida que los porcentajes de almidón en la dieta aumentaron. El cociente entre la actividad de amilasa y proteasa (A/P) se redujo en A. longinaris con los mayores porcentajes de almidón dietario; por el contrario, el cociente A/P en P. muelleri aumentó cuando la concentración fue más alta. Estos resultados demostraron que existe una estrecha relación entre los hábitos alimentarios y la fisiología digestiva de las dos especies estudiadas; sugiriendo un comportamiento más herbívoro para A. longinaris y más omnívoro para P. muelleri.

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Section: Introductionmentioning

confidence: 99%

Influence of dietary starch and cellulose levels on the metabolic profile and apparent digestibility in penaeoid shrimp

Velurtas¹,

Díaz²,

Fernández-Gimenez³

et al. 2011

lajar

View full text Add to dashboard Cite

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“…This point is supported by comparing the kinetic constants of sugars in the hepatopancreas and in the intestine. The K M for both D-glucose and D-fructose in the hepatopancreas is in the high M or low mM range in comparison to the low µM range in the intestine supporting a higher affinity binding protein (Ahearn et al, 1985;Verri et al, 2001).…”

Section: Comparative Sugar Transportmentioning

confidence: 89%

“…The foregut and hindgut are lined with a chitinous cuticle and are understood to play a minimal role in nutrient absorption compared to the midgut (Wright and Ahearn, 1997). Although the primary source of energy for crustaceans is not carbohydrates, the amount of carbohydrates available to crustaceans have a direct effect on growth and survival (Verri et al 2001). …”

Section: Figurementioning

confidence: 99%

“…The hepatopancreas is a major site of sugar absorption (Ahearn and Maginniss, 1977) and is located bilaterally in the thoracic cavity consisting of E, F, R, B, and M cells (Verri, et al 2001). The variety of cells in the hepatopancreas reflects the variety of hepatopancreatic functions in digestion and absorption.…”

Section: Figurementioning

confidence: 99%

“…The intestine is comprised of a single epithelial cell type with various transport proteins in the membranes of these cells and is considered to be a scavenger organ since it is secondary in nutrient absorption to the hepatopancreas (Wright and Ahearn, 1997). The intestine plays a significant role in the absorption of D-glucose and D-fructose (Verri et al, 2001 andObi et al, 2011). Carbohydrate digestion for these animals begins in the gastric chamber by secretion of a digestive enzyme mixture including α-amylase, glycosidases, maltase, and α-glucosidase (Johnson, 2003).…”

Section: Figurementioning

confidence: 99%

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Functional characterization of a putative disaccharide membrane transporter in crustacean intestine

2014

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Mechanisms of transepithelial absorption of dietary sucrose in the American lobster, Homarus americanus, were investigated to determine if disaccharide sugars can be transported across an animal gut intact. Intestines were mounted in a perfusion chamber to measure mucosal to serosal (MS) 14C‐sucrose transport. MS sucrose transport was a hyperbolic function of luminal sugar concentration, suggesting the presence of carrier‐mediated transport. Sodium‐dependent glucose transport inhibitor, phloridzin, partially decreased MS 14C‐sucrose transport, suggesting hydrolysis of 14C‐sucrose to glucose during transport. Phloretin, an inhibitor of Na+‐independent basolateral glucose transport, partially decreased MS 14C‐sucrose transport, suggesting that some 14C‐sucrose radioactivity may be transported to the blood as 14C‐glucose. Decreased MS 14C‐sucrose transport also occurred in the presence of the disaccharide trehalose. Thin‐layer chromatography (TLC) was used to identify the chemical nature of radioactively‐labeled sugars in the bath following transport and revealed that 14C‐sucrose was transported across the intestine largely as an intact molecule. Results suggest that disaccharide sugars may be transported intact across crustacean intestine and provide support for the occurrence of a disaccharide membrane transporter that has not previously been functionally characterized. Grant Funding Source: This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2010‐65

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Adaptation to hypoosmotic challenge in brachyuran crabs: a microanatomical and electrophysiological characterization of the intestinal epithelia

2005

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Besides its role in digestion and nutrient absorption, the crustacean gut participates in osmo/ionic regulation. We investigate microanatomy, ionic permeability and transepithelial electrophysiological parameters in the mid- and hindguts of three hyperosmoregulating crabs that inhabit estuarine waters (Chasmagnathus granulata), brackish mangrove swamp (Sesarma rectum) or freshwater (Dilocarcinus pagei). The abdominal hindguts are cuticle lined, the single-layered epithelia consisting of narrow, columnar cells exhibiting apically dense, unvesiculated cytoplasm. In the saltwater species, the thoracic midgut epithelium consists of tall, narrow, columnar cells displaying numerous, apical microvilli above dense apical cytoplasm. However, the corresponding gut segment in the hololimnetic species, D. pagei, consists of squat cells lacking apical microvilli, overlain by a heavy cuticle, constituting a thoracic or anterior hindgut. The midgut/thoracic hindgut epithelia in all three crabs, and abdominal (posterior) hindgut of D. pagei, exhibit similar, small, lumen-negative voltages when perfused symmetrically with hemolymph-like salines. The hindguts of the saltwater species show similar, small, lumen-positive voltages. Small short-circuit currents are detectable after voltage clamping. Washout and/or addition of luminal glucose or amino acids do not alter current or conductance, suggesting the absence of active, electrogenic nutrient absorption. Ion substitution did not disclose active, electrogenic absorption or secretion of Na+ and/or Cl-. The midguts of the saltwater species exhibit similar conductances, greater than in D. pagei, but no ion selectivity; hindgut conductance is low, the epithelia showing moderate anion selectivity. The thoracic (anterior) and abdominal (posterior) hindgut epithelia of D. pagei, the freshwater species, exhibit similar, low conductances, and are ion selective. These findings reveal that active, electrogenic, salt and nutrient transport is undetectably low or absent. The reduced transepithelial conductances and notable ion selectivities in the abdominal and thoracic hindguts of D. pagei may reduce passive salt losses in fresh water, contributing to osmotic and ionic regulation.

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D-glucose transport in decapod crustacean hepatopancreas

Cited by 30 publications

References 0 publications

Influence of dietary starch and cellulose levels on the metabolic profile and apparent digestibility in penaeoid shrimp

Influence of dietary starch and cellulose levels on the metabolic profile and apparent digestibility in penaeoid shrimp

Functional characterization of a putative disaccharide membrane transporter in crustacean intestine

Adaptation to hypoosmotic challenge in brachyuran crabs: a microanatomical and electrophysiological characterization of the intestinal epithelia

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