D-galactose-induced aging in rats – The effect of metformin on bioenergetics of brain, skeletal muscle and liver

Sumbalová, Zuzana; Uličná, O; Kucharská, J; Rausová, Zuzana; Vančová, Oľga; Melicherčík, Lubomír; Tvrdík, Tomáš; Nemec, Marek; Kašparová, Svatava

doi:10.1016/j.exger.2022.111770

Cited by 8 publications

(7 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The biochemical testing on the mice's liver and kidneys did not reveal any appreciable abnormalities. Previous findings (Khedr et al, 2022; Sumbalová et al, 2022; Zameer et al, 2021; Zeng et al, 2022) were partially supported by the behavioural experiment results, which showed that the older mice were more sad, had worse motor function, and had worsened learning and memory capacities. After receiving Rg1 therapy, this circumstance became better.…”

Section: Discussionmentioning

confidence: 69%

Ginsenoside Rg1 attenuates D‐galactose‐induced neural stem cell senescence via the Sirt1‐Nrf2‐BDNF pathway

Hu,

Ran,

Wang

et al. 2023

Eur J of Neuroscience

View full text Add to dashboard Cite

With the ageing of society's population, neurodegenerative diseases have become an important factor affecting the quality of life and mortality in the elderly. Since its physiopathological processes are complex and the authorized medications have recently been shown to have several adverse effects, the development of safe and efficient medications is urgently needed. In this study, we looked at how ginsenoside Rg1 works to postpone neural stem cell ageing and brain ageing, giving it a solid scientific foundation for use as a therapeutic therapy for neurodegenerative diseases.

show abstract

Section: Discussionmentioning

confidence: 69%

Ginsenoside Rg1 attenuates D‐galactose‐induced neural stem cell senescence via the Sirt1‐Nrf2‐BDNF pathway

Hu,

Ran,

Wang

et al. 2023

Eur J of Neuroscience

View full text Add to dashboard Cite

show abstract

“…D-galactose (D-gal)는 당단백질의 구성 요소로 유제 품, 과일 및 채소 등 다양한 식품에 풍부하게 존재하는 환원당의 일종으로 정상적인 농도에서는 포도당으로 대사된다 9) . 그러나 비정상적인 축적은 D-gal 산화효소 의 작용을 통해 노화 촉진에 기여해 [10][11][12] , 최종 당산화물 (advanced glycation end products, AGE) 생성을 증가시 켜서 세포 노화를 모방하기 때문에 본 연구에서 노화의 유도를 위해 D-gal을 사용하였다 10,11) . 물풀과(Lamiaceae family)에 속하는 한해살이 식물인 소엽(蘇葉, Perilla frutescens (L.) Britt.…”

Section: 서론»»»unclassified

“…D-gal의 비정상적인 축적은 AGE 생성을 증가시킨다 25) . 증가된 AGE는 노화 촉진에 기여하기 때문에, D-gal은 산화적 스트레스 매개 노화 연 구를 위한 모델로 널리 사용되고 있다 [10][11][12] . 이를 바탕으 43,44) .…”

Section: D-gal에 의한 C2c12 세포의 세포사멸 유도에 미치는 Eepf의 영향unclassified

See 1 more Smart Citation

Cellular Aging Inhibitory Effect of Perilla Leaf Extract on D-Galactose Induced C2C12 Myoblasts

Park,

Cho,

Choi

2024

J Korean Med Rehabil

View full text Add to dashboard Cite

Objectives We used the D-galactose (D-gal) induced C2C12 myoblast senescence model to investigate whether ethanol extract of Perilla. fructescens leaves (EEPF) could delay cellular senescence and regulate related mechanisms. Methods C2C12 myogenic cells were cultured in an incubator under 37 ℃ and 5% CO 2 conditions. EEPF, dried perilla leaves were pulverized and extracted at 1:10 (v/v) at 50 ℃ for 4 hours. Cell counting kit-8 and western blot analysis was performed. Annexin V-FITC apoptosis detection kit and DAPI staining was applied. Catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and malondialdehyde analysis kits were used. To measure the level of reactive oxygen species generation, staining and flow cytometry was used. To analyze the mitochondrial activity, membrane potential changes were measured using JC-1. β-gal activity was analyzed using SA-β-gal staining solution, and DNA damage was analyzed by using γ-H2AX. Quantikine ELISA kit was used to analyze inflammatory cytokine production. Results According to the results of this study, EEPF significantly alleviated the decrease in cell viability in C2C12 cells treated with D-gal and suppressed the decrease in the expression of proliferating cell nuclear antigen. EEPF also markedly blocked D-gal-induced C2C12 cell apoptosis and restored reduced activity of CAT, GSH-Px, T-AOC, SOD. In addition, EEPF suppressed the decrease in β-galactosidase activity, the induction of DNA damage and the increase in expression of senescence-associated secretory phenotype proteins such as p16, p53 and p21 in D-gal-treated C2C12 cells. Furthermore, EEPF significantly attenuated D-gal-induced production and expression of inflammatory cytokines such as interleukin (IL)-6 and IL-18.Conclusions The results of this study indicate that EEPF can be used as a potential candidate for the prevention and treatment of muscle aging.

show abstract

“…D-galactose has been widely used to induce cellular senescence and pathophysiological processes as ER stress [22][23][24][25][26][27][28][29][30][31][32] related to aging because it induces oxidative stress. However, it has been reported that different concentrations of D-galactose and exposure times are needed to induce senescence [24][25][26][27][28]33] and a different susceptibility and response of the same cell type in different species has also been observed [34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Curcumin on D-Galactose-Induced Senescence and Oxidative Stress in LLC-PK1 and HK-2 Cells

García-Trejo,

Gómez-Sierra,

Eugenio-Pérez

et al. 2024

Antioxidants

View full text Add to dashboard Cite

D-galactose has been widely used as an inducer of cellular senescence and pathophysiological processes related to aging because it induces oxidative stress. On the other hand, the consumption of antioxidants such as curcumin can be an effective strategy to prevent phenotypes related to the enhanced production of reactive oxygen species (ROS), such as aging and senescence. This study aimed to evaluate the potential protective effect of curcumin on senescence and oxidative stress and endoplasmic reticulum stress induced by D-galactose treatment in Lilly Laboratories Culture-Porcine Kidney 1 (LLC-PK1) and human kidney 2 (HK-2) proximal tubule cell lines from pig and human, respectively. For senescence induction, cells were treated with 300 mM D-galactose for 120 h and, to evaluate the protective effect of the antioxidant, cells were treated with 5 µM curcumin for 24 h and subsequently treated with curcumin + D-galactose for 120 h. In LLC-PK1 cells, curcumin treatment decreased by 20% the number of cells positive for senescence-associated (SA)-β-D-galactosidase staining and by 25% the expression of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and increased by 40% lamin B1 expression. In HK-2 cells, curcumin treatment increased by 60% the expression of proliferating cell nuclear antigen (PCNA, 50% Klotho levels, and 175% catalase activity. In both cell lines, this antioxidant decreased the production of ROS (20% decrease for LLC-PK1 and 10 to 20% for HK-2). These data suggest that curcumin treatment has a moderate protective effect on D-galactose-induced senescence in LLC-PK1 and HK-2 cells.

show abstract

D-galactose-induced aging in rats – The effect of metformin on bioenergetics of brain, skeletal muscle and liver

Cited by 8 publications

References 73 publications

Ginsenoside Rg1 attenuates D‐galactose‐induced neural stem cell senescence via the Sirt1‐Nrf2‐BDNF pathway

Ginsenoside Rg1 attenuates D‐galactose‐induced neural stem cell senescence via the Sirt1‐Nrf2‐BDNF pathway

Cellular Aging Inhibitory Effect of Perilla Leaf Extract on D-Galactose Induced C2C12 Myoblasts

Protective Effect of Curcumin on D-Galactose-Induced Senescence and Oxidative Stress in LLC-PK1 and HK-2 Cells

Contact Info

Product

Resources

About