d-Aspartate N-methyltransferase catalyzes biosynthesis of N-methyl-d-aspartate (NMDA), a well-known selective agonist of the NMDA receptor, in mice

Shibata, Keitaro; Imanishi, Daiki; Abe, Katsumasa; Suzuki, Masataka; Takahashi, Shouji; Kera, Yoshio

doi:10.1016/j.bbapap.2020.140527

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…NMDA is an important excitatory neurotransmitter L-glutamate homologue in the mammalian central nervous system [ 30 ]. Accumulating studies have revealed that NMDA can induce RGC degradation and retinal injury [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Baicalin suppresses glaucoma pathogenesis by regulating the PI3K/AKT signaling in vitro and in vivo

Zhao

Shi

et al. 2021

Bioengineered

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Glaucoma, characterized with progressive degeneration of retinal ganglion cells (RGCs), is the second frequently leading cause of sight loss in the word after cataract. Baicalin plays a protective role in age-related macular degeneration, retinopathy of prematurity, branch retinal vein occlusion, and ischemia-induced neurodegeneration in the retina. The present study aimed to investigate the role of baicalin in glaucoma. RGCs were stimulated with N-methyl-D-aspartate (NMDA) to mimic the in vitro model of glaucoma. A mouse model of glaucoma induced by chronic elevated intraocular pressure was also established. The apoptosis, oxidative stress, and autophagy of RGCs were detected by flow cytometry analysis, 2,7-dichlorodihydrofluorescein diacetate staining, and Western blotting, respectively. Retinal pathological changes were exhibited by hemotoxylin and eosin staining. Baicalin restrained the NMDA-induced cell apoptosis, autophagy, and oxidative stress of RGCs by activating the PI3K/AKT signaling in vitro . The elevated intraocular pressure-induced pathological changes in retinas of glaucoma mice were attenuated by baicalin. Moreover, the number of RGCs was significantly decreased in glaucoma mice, and then increased by baicalin treatment. Baicalin also inhibited autophagy and activated PI3K/AKT signaling in vivo . In conclusion, baicalin suppresses glaucoma pathogenesis by regulating the PI3K/AKT signaling in vitro and in vivo .

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Section: Discussionmentioning

confidence: 99%

Baicalin suppresses glaucoma pathogenesis by regulating the PI3K/AKT signaling in vitro and in vivo

Zhao

Shi

et al. 2021

Bioengineered

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“…Inhibitors of NAAG peptidase are investigated in ischemic damage and diseases of the nervous system, including schizophrenia, diabetic neuropathy, Alzheimer’s disease, and amyotrophic lateral sclerosis [ 10 ]. N-Methyl-D-aspartate (NMDA) is synthesized by an S-adenosyl-L-methionine-dependent enzyme from D-Asp [ 96 ]. The NMDA is a specific agonist of NMDA receptors that is used widely in neurological research [ 51 ].…”

Section: Metabolism and Physiologic Importance Of Aspartic Acidmentioning

confidence: 99%

“…N-Methyl-D-aspartate (NMDA) is synthesized by an S-adenosyl-L-methionine-dependent enzyme from D-Asp [ 96 ]. The NMDA is a specific agonist of NMDA receptors that is used widely in neurological research [ 51 ].…”

Section: Metabolism and Physiologic Importance Of Aspartic Acidmentioning

confidence: 99%

Aspartic Acid in Health and Disease

Holeček

2023

Nutrients

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Aspartic acid exists in L- and D-isoforms (L-Asp and D-Asp). Most L-Asp is synthesized by mitochondrial aspartate aminotransferase from oxaloacetate and glutamate acquired by glutamine deamidation, particularly in the liver and tumor cells, and transamination of branched-chain amino acids (BCAAs), particularly in muscles. The main source of D-Asp is the racemization of L-Asp. L-Asp transported via aspartate–glutamate carrier to the cytosol is used in protein and nucleotide synthesis, gluconeogenesis, urea, and purine-nucleotide cycles, and neurotransmission and via the malate–aspartate shuttle maintains NADH delivery to mitochondria and redox balance. L-Asp released from neurons connects with the glutamate–glutamine cycle and ensures glycolysis and ammonia detoxification in astrocytes. D-Asp has a role in brain development and hypothalamus regulation. The hereditary disorders in L-Asp metabolism include citrullinemia, asparagine synthetase deficiency, Canavan disease, and dicarboxylic aminoaciduria. L-Asp plays a role in the pathogenesis of psychiatric and neurologic disorders and alterations in BCAA levels in diabetes and hyperammonemia. Further research is needed to examine the targeting of L-Asp metabolism as a strategy to fight cancer, the use of L-Asp as a dietary supplement, and the risks of increased L-Asp consumption. The role of D-Asp in the brain warrants studies on its therapeutic potential in psychiatric and neurologic disorders.

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Improvement of Aspartate-Signal Fitting Accuracy in Asp-Edited MEGA-PRESS Spectra

et al. 2023

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d-Aspartate N-methyltransferase catalyzes biosynthesis of N-methyl-d-aspartate (NMDA), a well-known selective agonist of the NMDA receptor, in mice

Cited by 4 publications

References 34 publications

Baicalin suppresses glaucoma pathogenesis by regulating the PI3K/AKT signaling in vitro and in vivo

Baicalin suppresses glaucoma pathogenesis by regulating the PI3K/AKT signaling in vitro and in vivo

Aspartic Acid in Health and Disease

Improvement of Aspartate-Signal Fitting Accuracy in Asp-Edited MEGA-PRESS Spectra

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