d-alanine interference in microbiological assays of vitamin B6 in human blood

Haskell, Betty E.; Wallnöfer, Ute

doi:10.1016/0003-2697(67)90248-5

Cited by 6 publications

(2 citation statements)

References 25 publications

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“…Be-dependent assay organisms have not been reported. The problems encountered in applying microbiological procedures to the determi nation of vitamin B6 in blood have been discussed by Storvick and Peters (7) and by Haskell and WallnÃ ¶fer (8). Blood ap pears to contain growth-promoting, or inhibitory substances, or both, for the various organisms that invalidate use of the differential technique.…”

Section: Benson Peters Edwards Malinow and Storvickmentioning

confidence: 99%

“…The limitations of the 3 organisms, utilizing the differential tech nique with regard to the assay of blood, have been pointed out (7,8). Therefore, it must be recognized that vitamin B8 values for blood, particularly for samples drawn before supplementation, are prob ably not valid due to the very small amount of vitamin B6 present in relationship to interfering substances.…”

Section: Rhesus Monkeys Response To a Vitaminmentioning

confidence: 99%

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Vitamin B6 in Blood, Urine, and Liver of Monkeys

Benson

Peters

Edwards

et al. 1968

The Journal of Nutrition

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Blood and urinary vitamin Be and urinary 4-pyridoxic acid values are reported for 3 rhesus monkeys before and after a 5-day period of pyridoxol-HCl sup plementation. Blood and liver vitamin Be values are given for a group of free-ranging howler monkeys. Microbiological procedures employing Saccharomyces carlsbergensis ATCC 9080, Streptococcus faecium 0 51 NCDO 1229 and Lactobacillus casei ATCC 7469 were used for the vitamin B8 analyses and a microprocedure of the lactone method was used for 4-pyridoxic acid determinations. Large increments of vitamin Be in blood and urine and increased excretion of 4-pyridoxic acid were observed in the rhesus monkeys following supplementation. Of significance was the prolonged elevation of values several days after supplementation had ceased. Blood values for the howler monkeys were higher than those for the rhesus. Differential assay of liver hydrolysates from howler monkeys revealed that most of the vitamin was present in the pyridoxamine form. A lesser amount was in the pyridoxal form and the amount of pyridoxol was negligible.

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Section: Benson Peters Edwards Malinow and Storvickmentioning

confidence: 99%

Section: Rhesus Monkeys Response To a Vitaminmentioning

confidence: 99%