Cytotoxicity Test of Cocoa Leaf Ethanol Extract (Theobroma Cacao L.) With Brine Shrimp Lethality Test (BSLT) Method

Rani, Zulmai; Ridwanto, Ridwanto; Miswanda, Dikki; Yuniarti, Rafita; Sutiani, Ani; Syahputra, Ricky Andi; Irma, Reza

doi:10.24114/ijcst.v5i2.37452

Cited by 14 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…By entering the value (50% probit of death of the test animals) as y, the results of x are obtained as the log concentration value. The antilog of the x value is the LC50 value (Rani, Z., et al 2022).…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic Test of Ethanol Extract of Bintaro Fruit and Peel (Cerbera odollam Gaertn.) against Artemia salina Leach Larvae

Handayani¹,

Syarif²,

Rahma³

2023

ijcsrr

View full text Add to dashboard Cite

Bintaro (Cerbera odollam Gaertn). Plants belonging to the Apocynaceae family have properties such as antifungal, insecticide, antioxidant and antitumor. Bintaro fruit contains alkaloids, flavonoids, terpenoids, tannins and saponins. Bintaro plants are poisonous because they contain alkaloid group compounds which are toxic. This study aims to obtain data and information regarding the toxicity ratio of bintaro rind and bintaro fruit (Cerbera odollam Gaertn.) to Artemia salina Leach larvae by determining the value of Lethal Concentration 50 (LC50). This type of research was experimental with quantitative methods, using Artemia salina Leach larvae. Each extract was divided into 4 concentration groups. In the ethanol extract of bintaro fruit peel, namely at concentrations of 500, 1000, 1500 and 2500 ppm. Meanwhile, bintaro fruit extract at concentrations of 1000, 1500, 2000 and 2500 ppm, each of which was carried out 3 replications with 10 larvae. Data analysis using probit analysis showed that the ethanol extract of bintaro fruit peel (Cerbera odollam Gaertn.) had LC50 = 701.455 μg/mL which was categorized as toxic. Meanwhile, the ethanol extract of bintaro fruit (Cerbera odollam Gaertn.) has LC50 = 1199.5 μg/mL which is categorized as nontoxic.

show abstract

Section: Resultsmentioning

confidence: 99%

Cytotoxic Test of Ethanol Extract of Bintaro Fruit and Peel (Cerbera odollam Gaertn.) against Artemia salina Leach Larvae

Handayani¹,

Syarif²,

Rahma³

2023

ijcsrr

View full text Add to dashboard Cite

show abstract

“…Setelah sudah 5 hari saring menggunakan kertas saring, kumpulkan maserat (maserat I), lalu residu direndam kembali dengan 1250 ml etanol 70% selama 2 hari (Nurmazela et al, 2022). Lakukan penyaringan (maserat II), maserat I dan II kemudian di uapkan menggunakan rotary evaporator, sampai di peroleh ekstrak yang kental (Rani et al, 2022).…”

Section: Pembuatan Ekstrakunclassified

FORMULASI SEDIAAN GEL EKSTRAK KULIT BATANG ASAM KANDIS (Garcinia xanthochymus Hook.f. ex. Anderson) DAN UJI ANTIINFLAMASI TERHADAP TIKUS PUTIH JANTAN

Septiana,

Winata,

Panggabean

et al. 2024

View full text Add to dashboard Cite

Penggunaan ekstrak kulit buah asam kandis sebagai antiinflamasi belum maksimal karena harus disiapkan dan dioleskan langsung dalam bentuk utuh membuatnya kurang praktis. Oleh karena itu, harus dikembangkan menjadi sediaan farmasi untuk membuatnya lebih mudah digunakan dan praktis. Tujuan penelitian ini adalah untuk mengetahui bagaimana membuat gel ekstrak kulit batang asam kandis (Garcinia Xanthochymus Hook.F. Ex. Anderson) dan melakukan uji antiinflamasi pada tikus putih jantan. Penelitian ini menggunakan metode eksperimental untuk mengidentifikasi dan menggambarkan tanaman, membuat ekstrak kulit batang asam kandis secara maserasi, membuat sediaan gel, dan menguji efek antiinflamasi ekstrak kulit batang asam kandis pada tikus putih jantan. Analisis data berdasarkan hasil observasi dilanjutkan dengan analisis dan statistik menggunakan Anova. Hasil penelitian yaitu ekstrak kulit batang asam kandis dengan konsenterasi 5, 10, dan 15% dengan gelling agent carbopol 940 dapat dibuat sediaan gel antiinflamasi. Sediaan gel antiinflamasi yang terbuat dari ekstrak kulit batang asam kandis telah memenuhi persyaratan yang baik dan stabil selama proses uji coba roda gigi pada organoleptis, homogenitas, pH, dan viskositas. Konsenterasi terbaik gel ekstrak kulit batang asam kandis yang memberikan efek antiinflamasi yaitu konsenterasi 15%. Diharapkan kepada peneliti selanjutnya agar melakukan penelitian ekstrak kulit batang asam kandis dengan jenis sediaan yang berbeda.

show abstract

“…Sebanyak 10 g serbuk simplisia dan ekstrak ditimbang lalu ditambahkan 100 ml air suling panas, didihkan selama 5 menit dan disaring dalam keadaan panas, kedalam 5 ml filtrat ditambahkan serbuk magnesium dan 1 ml HCl pekat dan 2 ml amil alkohol, dikocok kuat dan biarkan memisah. Adanya flavonoid ditandai dengan adanya warna merah, kuning atau jingga pada lapisan amil alkohol (Rani et al, 2022).…”

Section: Pemeriksaan Flavonoidunclassified

Penentuan Kadar Alkaloid Total Ektstrak Akar Bajakah (Spatholobus Littoralis Hassk) dengan Metode Spektofotometri Uv-Vis

Hanifah,

Daulay,

Rahman

et al. 2024

jharma

View full text Add to dashboard Cite

The Bajakah tampala tree (Spatholobus littoral hassk) is a native plant from the interior of the forests in Kalimantan, especially Central Kalimantan. This plant is a plant that stretches or grows and grows abundantly in the interior of the forest. The Bajakah plant is known to the people of Kalimantan as a medicinal plant and can cure various diseases, including diarrhea, rheumatism, and cancer. The aim of this research was to determine the alkaloid content of bajakah root extract in various concentrations. The research method was carried out experimentally, with the independent variable being an ethanol extract of bajakah roots with concentrations of 50, 70, and 96%. Meanwhile, the dependent variables are the characteristics of simplicia, the secondary metabolite content of Bajakah root ethanol extract, and the determination of the total alkaloid content of Bajakah root extract. Bajakah root simplicia extract is made with 96, 70, and 50% ethanol solvents. The parameters used in this study include water content, total ash content, acid insoluble ash content, water soluble extract content, ethanol soluble extract content, and secondary metabolite content, including alkaloids, tannins, flavonoids, saponins, steroids, and terpenoids, by conducting phytochemical screening. , making a standard caffeine solution, determining the maximum wavelength of caffeine, measuring the caffeine calibration curve, and determining the total alkaloid content of Bajakah root extract. The results of the phytochemical screening showed that Bajakah root was positive for containing alkaloid compounds, saponins, tannins, flavonoids, steroids, and terpenoids. And the results obtained from Bajakah root plants obtained from Central Kalimantan were a 96% ethanol concentration of 1.66797 ± 0.7088 mg/gram, a 70% ethanol concentration of 1.6679 ± 0.1124 mg/gram, and a 50% ethanol concentration of 0.46981 ± 1.0656 mg/gram

show abstract

Cytotoxicity Test of Cocoa Leaf Ethanol Extract (Theobroma Cacao L.) With Brine Shrimp Lethality Test (BSLT) Method

Cited by 14 publications

References 0 publications

Cytotoxic Test of Ethanol Extract of Bintaro Fruit and Peel (Cerbera odollam Gaertn.) against Artemia salina Leach Larvae

Cytotoxic Test of Ethanol Extract of Bintaro Fruit and Peel (Cerbera odollam Gaertn.) against Artemia salina Leach Larvae

FORMULASI SEDIAAN GEL EKSTRAK KULIT BATANG ASAM KANDIS (Garcinia xanthochymus Hook.f. ex. Anderson) DAN UJI ANTIINFLAMASI TERHADAP TIKUS PUTIH JANTAN

Penentuan Kadar Alkaloid Total Ektstrak Akar Bajakah (Spatholobus Littoralis Hassk) dengan Metode Spektofotometri Uv-Vis

Contact Info

Product

Resources

About