Cytotoxicity screening of Thymus vulgaris L. essential oil in brine shrimp nauplii and cancer cell lines

Nikšić, Haris; Bečić, Fahir; Koric, Emina; Gusic, Irma; Omeragić, Elma; Muratović, Salko; Miladinović, Bojana; Durić, Kemal

doi:10.1038/s41598-021-92679-x

Cited by 73 publications

(47 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides antimicrobial activity, cytotoxic effects of some EOs viz., lemon verbena ( Lippia citriodora Palau. Kunth) 23 , thyme ( Thymus vulgaris L.) 24 and rosemary ( R. officinalis ) 25 have been reported earlier against some tumor cell lines.…”

Section: Introductionmentioning

confidence: 92%

Seasonal variability in essential oil composition and biological activity of Rosmarinus officinalis L. accessions in the western Himalaya

Rathore

Mukhia

Kapoor

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Rosmarinus officinalis L. is an imperative herb used in pharmaceutical yet knowledge on chemical and activity profile of essential oil (EO) to harvest seasons and accessions from the Himalayan region is limited. Thus, accessions were evaluated to determine the EO content, compositional, antimicrobial, and cytotoxic potential of rosemary in different harvest seasons during 2018‒2019. EO content was 30.5% higher in IHBT/RMAc-1 compared with IHBT/RMAc-2 accession while 27.9% and 41.6% higher in the autumn as compared with summer and rainy season, respectively. Major EO compound was 1,8-cineole; ranged from 32.50‒51.79% during harvest seasons and 38.70‒42.20% in accessions. EO was active against both the tested Gram-positive bacteria (Micrococcus luteus MTCC 2470 and Staphylococcus aureus MTCC 96). EOs showed inhibition of Gram-negative bacteria (Salmonella typhi MTCC 733), while Klebsiella pneumoniae MTCC 109 was found to be resistant. The rosemary EO of T1 (Rainy season IHBT/RMAc-1) was most effective against S. aureus MTCC 96 with the minimum inhibitory concentration (MIC) of 4% (v/v). In vitro cytotoxicity evaluation showed no potential anti-proliferative activity of EO. The rosemary EO profile in the western Himalayan region was influenced by harvesting seasons and genetic variability within the accessions; furthermore, a promising antibacterial agent in pharmaceutical and flavour industries.

show abstract

Section: Introductionmentioning

confidence: 92%

Seasonal variability in essential oil composition and biological activity of Rosmarinus officinalis L. accessions in the western Himalaya

Rathore

Mukhia

Kapoor

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…While the BSLA serves as an excellent tool to identify toxicity, a more sensitive distinction of activity is the testing against cell lines [ 53 ]. Hence, the BSLA was used to identify combinations of interest, which were then followed by the MTT assay using RAW 264.7 macrophages and A549 lung cell lines to substantiate the results of the BSLA as the latter study considers the metabolic activity of the cells.…”

Section: Resultsmentioning

confidence: 99%

Essential Oil Blends: The Potential of Combined Use for Respiratory Tract Infections

2021

View full text Add to dashboard Cite

This study investigated the potential efficacy of 369 commercial essential oil combinations for antimicrobial, anti-toxic and anti-inflammatory activity with the aim of identifying synergy among essential oils commonly used in combination by aromatherapists for respiratory purposes. Essential oil combinations were assessed for their antimicrobial activities using a panel of Gram-positive, Gram-negative, and yeast strains associated with respiratory tract infections. The antimicrobial activity was measured by determining the minimal inhibitory concentration (MIC) of microbial growth. The fractional inhibitory concentration index (ΣFIC) was calculated to determine the antimicrobial interactions between the essential oils in the combination. The toxicity of the essential oil combinations was tested in vitro using the brine shrimp lethality assay, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on RAW 264.7 mouse macrophage cells and A549 lung cancer cell lines. In addition, an inflammatory response was evaluated measuring nitric oxide production. The essential oils, when in combination, demonstrated an increased antimicrobial effect, a reduction in toxicity and provided improved anti-inflammatory outcomes. Five distinct combinations [Cupressus sempervirens (cypress) in combination with Melaleuca alternifolia (tea tree), Hyssopus officinalis (hyssop) in combination with Rosmarinus officinalis (rosemary), Origanum marjorana (marjoram) in combination with M. alternifolia, Myrtus communis (myrtle) in combination with M. alternifolia and Origanum vulgare (origanum) in combination with M. alternifolia] were found to be the most promising, demonstrating antimicrobial activity, reduced cytotoxicity and improved anti-inflammatory effects. With the increased prevalence of respiratory tract infections and the growing antimicrobial resistance development associated with antimicrobial treatments, this study provides a promising complementary alternative for the appropriate use of a selection of essential oil combinations for use in the respiratory tract.

show abstract

“…In similar tests with the same cell line, observed in the literature, concentrations <100% demonstrated similar behavior and were generally null [ 26 , 27 ]; therefore, we used higher concentrations, so that we visualized well-defined behavior characteristics of a dose-dependent response [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

A Potential New Source of Therapeutic Agents for the Treatment of Mucocutaneous Leishmaniasis: The Essential Oil of Rhaphiodon echinus

et al. 2022

View full text Add to dashboard Cite

Weeds are an important source of natural products; with promising biological activity. This study investigated the anti-kinetoplastida potential (in vitro) to evaluate the cytotoxicity (in vitro) and antioxidant capacity of the essential oil of Rhaphiodon echinus (EORe), which is an infesting plant species. The essential oil was analyzed by GC/MS. The antioxidant capacity was evaluated by reduction of the DPPH radical and Fe3+ ion. The clone Trypanosoma cruzi CL-B5 was used to search for anti-epimastigote activity. Antileishmanial activity was determined using promastigotes of Leishmania braziliensis (MHOM/CW/88/UA301). NCTC 929 fibroblasts were used for the cytotoxicity test. The results showed that the main constituent of the essential oil was γ-elemene. No relevant effect was observed concerning the ability to reduce the DPPH radical; only at the concentration of 480 μg/mL did the essential oil demonstrate a high reduction of Fe3+ power. The oil was active against L. brasiliensis promastigotes; but not against the epimastigote form of T. cruzi. Cytotoxicity for mammalian cells was low at the active concentration capable of killing more than 70% of promastigote forms. The results revealed that the essential oil of R. echinus showed activity against L. brasiliensis; positioning itself as a promising agent for antileishmanial therapies.

show abstract

Cytotoxicity screening of Thymus vulgaris L. essential oil in brine shrimp nauplii and cancer cell lines

Cited by 73 publications

References 34 publications

Seasonal variability in essential oil composition and biological activity of Rosmarinus officinalis L. accessions in the western Himalaya

Seasonal variability in essential oil composition and biological activity of Rosmarinus officinalis L. accessions in the western Himalaya

Essential Oil Blends: The Potential of Combined Use for Respiratory Tract Infections

A Potential New Source of Therapeutic Agents for the Treatment of Mucocutaneous Leishmaniasis: The Essential Oil of Rhaphiodon echinus

Contact Info

Product

Resources

About