Cytotoxicity, Proliferation and Migration Rate Assessments of Human Dermal Fibroblast Adult Cells using Zingiber zerumbet Extract

Latif, Mazlyzam Abdul; Ibrahim, Farah Wahida; Arshad, Siti Aisyah; Chua, Kien Hui; Jufri, Nurul Farhana; Hamid, Asmah

doi:10.17576/jsm-2019-4801-14

Cited by 8 publications

(8 citation statements)

References 25 publications

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“…cells were seeded at a density of 5 × 10 3 cells/well in 96well plates for 24 h, in 200 μL of RPMI with 10% FBS. Then, the culture supernatant was removed and RPMI containing root extract of S. heyneanus of various concentrations of 10-500 μg/mL was added and incubated for 48 h. After treatment, the cells were incubated with MTT (10 μL, 5 mg/mL) at 37°C for 4 h and then with DMSO at room temperature for 1 h. The plates were read at 595 nm on a scanning multi-well spectrophotometer [20]. Data are represented as the mean values for three independent experiments:…”

Section: Mtt Assaymentioning

confidence: 99%

Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

Sundaram¹,

Sadhasivam²,

Sivaraj³

et al. 2021

Futur J Pharm Sci

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Background Strobilanthes heyneanus (Nilgirianthus heyneanus) belongs to the family Acanthaceae that contains many species with potential for diverse medicinal uses. It is also called ‘Karun kurinji’ and is commonly found in the South-West regions of India. The species are commonly used in rheumatic complaints, sprain of the ankle, and hernia. The objectives of the study were to evaluate the antioxidant activity, phytochemical analysis, and antibacterial activities of the root extract of S. heyneanus. Results The radical scavenging and reduction assays such as DPPH● radical and OH● radical scavenging assays, as well as phosphomolybdenum reduction and Fe3+ reducing power assays, were determined for the root extract. The highest DPPH● radical scavenging activity was 88.23 ± 1.32 at 120 μg/mL concentration, and the calculated IC50 was 38.52 μg/mL concentration. The highest OH● radical scavenging activity was 51.28 ± 1.06 at 120 μg/mL concentration, and the calculated IC50 was 51.28 μg/mL concentration. The highest ABTS●+ radical scavenging activity was 91.28 ± 1.12 at 30 μg/mL concentration, and the calculated IC50 was 33.92 μg/mL concentration. The highest phosphomolybdenum reduction was 87.43 ± 0.90 at 120 μg/mL concentration, and the calculated RC50 was 24.74 μg/mL concentration. The highest Fe3+ reduction was 89.38 ± 0.98 at 120 μg/mL concentration, and the calculated RC50 was 31.06 μg/mL concentration. The antibacterial activity of S. heyneanus showed the highest zone of inhibition of 24 mm for Salmonella typhi with 500 μg/mL concentration. The cytotoxicity limits of the root extracts were tested by MTT assay using human dermal fibroblast cell lines, reflecting > 90% cell viability at a concentration of 500 μg/mL. Conclusions The current study showed that the root extract of S. heyneanus has better antioxidant properties and potential anti-bacterial compounds. The phytochemical analysis of the root extract showed the presence of alkaloids, steroids, terpenoids, phenols, flavonoids, tannins, saponins, glycosides, and carbohydrates, which are responsible for the antibacterial root extract of S. heyneanus synergistically.

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Section: Mtt Assaymentioning

confidence: 99%

Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

Sundaram¹,

Sadhasivam²,

Sivaraj³

et al. 2021

Futur J Pharm Sci

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“…In wound healing condition, failure of myofibroblast to undergo apoptosis or convert back to the inactivated form of fibroblast would lead to excessive production of extracellular matrix (Latif et al, 2019), which contribute to the progression of fibrotic diseases (Ho et al, 2014). Uncontrolled activation of stiffnessinduced myofibroblast results in pathological fibrosis (Kuehl and Lagares, 2018).…”

Section: Role Of Navitoclax In Fibrosis/ Fibrotic Diseasesmentioning

confidence: 99%

Clinical Review: Navitoclax as a Pro-Apoptotic and Anti-Fibrotic Agent

et al. 2020

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B-cell lymphoma 2 (BCL-2) family proteins primarily work as a programmed cell death regulator, whereby multiple interactions between them determine cell survival. This explains the two major classes of BCL-2 proteins which are anti-apoptotic and pro-apoptotic proteins. The anti-apoptotic proteins are attractive targets for BCL-2 family inhibitors, which result in the augmentation of the intrinsic apoptotic pathway. BCL-2 family inhibitors have been studied extensively for novel targeted therapies in various cancer types, fibrotic diseases, aging-related as well as autoimmune diseases. Navitoclax is one of them and it has been discovered to have a high affinity toward BCL-2 anti-apoptotic proteins, including BCL-2, BCL-W and B-cell lymphoma-extra-large. Navitoclax has been demonstrated as a single agent or in combination with other drugs to successfully ameliorate tumor progression and fibrosis development. To date, navitoclax has entered phase I and phase II clinical studies. Navitoclax alone potently treats small cell lung cancer and acute lymphocytic leukemia, whilst in combination therapy for solid tumors, it enhances the therapeutic effect of other chemotherapeutic agents. A low platelet count has always associated with single navitoclax treatments, though this effect is tolerable. Moreover, the efficacy of navitoclax is determined by the expression of several BCL-2 family members. Here, we elucidate the complex mechanisms of navitoclax as a pro-apoptotic agent, and review the early and current clinical studies of navitoclax alone as well as with other drugs. Additionally, some suggestions on the development of navitoclax clinical studies are presented in the future prospects section.

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“…Basically, the incubation periods for the MTT assay were 24, 48, or 72 h. However, tumor cells are very heterogenous and their doubling times vary. Therefore, a 24 h incubation period was enough if the doubling time was ~16–24 h. However, for certain cancer cell lines, a 24 h incubation period with a given test substance is too short to demonstrate a significant effect on cell viability ( Abdul Latif et al, 2019 ; Alizadeh-Navaei et al, 2016 ; Guo et al, 2015 ). Therefore, in the present study, a 72 h incubation period was used to assess cell toxicity.…”

Section: Methodsmentioning

confidence: 99%

Apis mellifera propolis enhances apoptosis and invasion inhibition in head and neck cancer cells

Niyomtham

Koontongkaew

Yingyongnarongkul

et al. 2021

PeerJ

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Background Propolis is a resinous product accumulated from several plant sources that possess a wide range of therapeutic properties, including anti-cancer activities. However, the role of honeybee-produced propolis on head and neck squamous carcinoma (HNSCC) is not well understood. The aim of this study was to investigate the effects of Apis mellifera propolis on apoptosis and invasiveness in HNSCC cell lines. Methods Ethyl acetate extract of propolis (EAEP) was prepared from A. mellifera beehives using liquid–liquid extraction. High-performance liquid chromatography coupled with electrospray ionization-time of flight-mass spectrometry (HPLC-ESI-TOF-MS) was used to determine the flavonoids in EAEP. Isogenic HNSCC cell lines derived from primary (HN30 and HN4) and metastatic site (HN31 and HN12) were used in this study. The cytotoxicity, apoptosis, invasion, and MMP activity of EAEP on HNSCC cells were determined using an MTT assay, flow cytometry, Matrigel invasion assay, and gelatinase zymography, respectively. Results We found that EAEP exhibited cytotoxic activity and induced apoptosis in the HNSCC cell lines. Furthermore, EAEP significantly decreased HNSCC cell invasion by reducing MMP-2 and MMP-9 activity. Two flavonoids, galangin and apigenin, were identified in EAEP by HPLC-ESI-TOF-MS. The results suggest that EAEP promotes apoptosis and exerts anti-invasion potential by inhibiting MMP-2 and MMP-9 activity in HNSCC cell lines. These inhibitory effects may be mediated by galangin and apigenin.

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Cytotoxicity, Proliferation and Migration Rate Assessments of Human Dermal Fibroblast Adult Cells using Zingiber zerumbet Extract

Cited by 8 publications

References 25 publications

Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

Clinical Review: Navitoclax as a Pro-Apoptotic and Anti-Fibrotic Agent

Apis mellifera propolis enhances apoptosis and invasion inhibition in head and neck cancer cells

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