Cytotoxicity, oral toxicity, genotoxicity, and mutagenicity evaluation of essential oil from Psidium glaziovianum Kiaersk leaves

Costa, Wêndeo Kennedy; Nascimento, Matheus Ferreira do; Barbosa, Édipo Lucas Soares; Souza, Talita Giselly dos Santos; Chagas, Cristiano Aparecido; Napoleão, Thiago Henrique; Correia, Maria Tereza dos Santos; Brayner, Fábio André; Oliveira, Alisson Macário de; Silva, Márcia Vanusa da

doi:10.1016/j.jep.2022.115955

Cited by 8 publications

(4 citation statements)

References 39 publications

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“…Previously PgEO was evaluated for safety in use, the rate of hemolysis showed that PgEO has a limited ability to lyse cells (0.33-1.78%). Oral administration with up to 5,000 mg/kg did not show mortality or physiological abnormalities during the investigation of acute toxicity (Costa et al, 2023).…”

Section: Resultsmentioning

confidence: 84%

“…Psidium glaziovianum Kiaersk it is a small to medium-sized tree that is found in the Caatinga and Atlantic Forests of Brazil and is thought to be an endemic species there (Landrum et al, 2017). P. glaziovianum essential oil (PgEO) was previously reported to have antinociceptive and anti-in ammatory activity as well as low oral toxicity (Costa et al, 2022(Costa et al, , 2023.…”

Section: Introductionmentioning

confidence: 99%

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Development of gel containing Psidium glaziovianum essential oil has in vitro antimicrobial activity and improves healing of excisional wounds in mice

Costa

Guimarães

Silva

et al. 2023

Preprint

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Wounds are physical, chemical, biological or even medical procedure-related damage to the skin or mucous membranes. One of the major problems in the treatment of wounds is the development of infections, as they cause systemic damage to the patient, delaying recovery from the injury. In order to accelerate the healing process, there is currently an interest in finding drugs that can interact with the injured tissue and fight the associated infections. Previously P. glaziovianum essential oil (PgEO) has been shown to have antinociceptive and anti-inflammatory properties, as well as minimal oral toxicity. Therefore, our objective was to evaluate the impact of topical application of a gel formulation containing PgEO in excisional wounds in mice. Additionally, in vitro antimicrobial evaluation was also performed. The results of this research demonstrate that the gel containing essential oil of P. glaziovianum has action against gram-positive and gram-negative bacteria, as well as fungi. Furthermore, the results show that PgEO gel therapy improves skin wound healing, presumably because of its ability to stimulate antioxidant enzymes and inhibit pro-inflammatory cytokines. The gel also demonstrated minimal erythrocyte toxicity and skin irritation, which may hold promise for future preclinical and clinical studies in various types of wounds.

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Section: Resultsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Development of gel containing Psidium glaziovianum essential oil has in vitro antimicrobial activity and improves healing of excisional wounds in mice

Costa

Guimarães

Silva

et al. 2023

Preprint

Self Cite

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“…Initially, they were washed with neutral detergent and distilled water, then bathed in 70% alcohol and deposited in an oven (80 °C) for 15 min. Whilst still being heated, 10 μL of acridine orange (1 mg/mL) was uniformly spread on each slide, then placed to dry at room temperature (minimum 30 min) [ 37 ]. Before MN, the cytotoxicity of the treatments was analyzed.…”

Section: Methodsmentioning

confidence: 99%

Anti-Hyperuricemic, Anti-Arthritic, Hemolytic Activity and Therapeutic Safety of Glycoconjugated Triazole-Phthalimides

Silva,

Aires,

Cunha

et al. 2023

Biomedicines

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Hyperuricemia, the metabolic alteration that leads to gout or gouty arthritis, is increasing worldwide. Glycoconjugated triazole-phthalimides show potent anti-inflammatory activity. The aim of this study was to evaluate the anti-hyperuricemia effect of glycoconjugated triazole-phthalimides. To develop hyperuricemia, groups of mice received orally potassium oxonate (250 mg/kg) for 7 days, and F2, F3 and F4 glycoconjugated triazole-phthalimides (20 mg/kg), allopurinol (300 mg/kg), and 1% carboxymethylcellulose; indomethacin (2 and 4 mg/kg) was the positive control for anti-arthritic effect. Genotoxic and mutagenic effects were evaluated by the comet and micronucleus assays, respectively. The hemolytic action of the compounds was evaluated. Phthalimides F2, F3 and F4 significantly reduced the levels of serum uric acid, creatinine and urea in hyperuricemic animals. In addition, the compounds were efficient in reducing protein denaturation in a dose-dependent manner. In an interesting way, the histopathological analysis of kidneys from groups treated with F2, F3 and F4 showed a glomerular architecture, with the Bowman’s capsule and renal tubules having a normal appearance and without inflammatory changes. Also, F2 and F4 showed a small increase in micronuclei, indicating a low mutagenic effect, whilst by comet assay only, we could infer that F4 affected the frequency and damage index, thus indicating a very small genotoxic action. Similarly, the phthalimides showed a low degree of erythrocyte hemolysis (<3%). Our data demonstrate that the new glycoconjugate triazole-phthalimides have potential to treat hyperuricemia and its secondary complications, such as gouty arthritis, with a low to non-significant rate of erythrocytes hemolysis, genotoxicity and mutagenicity making these molecules strong candidates as pharmaceutical agents for treatment requiring uric-acid-lowering therapy.

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“…With distribution in tropical and subtropical regions of the world, traditional populations mention the genus Psidium as a resource in folk medicine to treat various diseases related to the digestive system, such as diarrhea, stomach pains, abdominal pain, and dysentery [12][13][14][15][16][17]. In addition, this genus is also used in the treatment of disorders of the genitourinary system [18] and the respiratory system, such as sore throat [16,19].…”

Section: Introductionmentioning

confidence: 99%

Essential Oil of Psidium glaziovianum Kiaersk Alleviates the Effects of Complete Freund’s Adjuvant (CFA)-Induced Arthritis by Regulating Inflammation and Oxidative Stress

Costa,

Alves,

Fonseca

et al. 2024

DDC

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Rheumatoid arthritis (RA) is a chronic and debilitating condition that affects a significant number of individuals worldwide. Unfortunately, the currently available therapeutic approaches often yield unsatisfactory results and may be accompanied by harmful side effects. A medicinal plant called Psidium glaziovianum Kiaersk has potential benefits in the treatment of this condition due to its anti-inflammatory and analgesic properties. In this study, our objective was to investigate the potential therapeutic effects of P. glaziovianum essential oil (PgEO) in alleviating arthritis symptoms in mice induced by Complete Freund’s Adjuvant (CFA). The effect of P. glaziovianum essential oil was evaluated in mice with Complete Freund’s Adjuvant (CFA)-induced arthritis. Edema sizes, macroscopic and radiographic images, cytokine levels, and oxidative stress were evaluated. Administration of PgEO at dosages of 50 and 100 mg/kg effectively prevented CFA-induced osteoarticular changes in arthritic mice, resulting in a significant reduction in joint damage. Additionally, the PgEO treatment exhibited the ability to minimize edema, a common symptom associated with arthritis. Furthermore, PgEO can modulate the levels of pro-inflammatory cytokines and oxidative stress, both of which play crucial roles in the progression of the disease. In conclusion, our study suggests that PgEO holds great potential as a natural therapeutic agent for rheumatoid arthritis.

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Cytotoxicity, oral toxicity, genotoxicity, and mutagenicity evaluation of essential oil from Psidium glaziovianum Kiaersk leaves

Cited by 8 publications

References 39 publications

Development of gel containing Psidium glaziovianum essential oil has in vitro antimicrobial activity and improves healing of excisional wounds in mice

Development of gel containing Psidium glaziovianum essential oil has in vitro antimicrobial activity and improves healing of excisional wounds in mice

Anti-Hyperuricemic, Anti-Arthritic, Hemolytic Activity and Therapeutic Safety of Glycoconjugated Triazole-Phthalimides

Essential Oil of Psidium glaziovianum Kiaersk Alleviates the Effects of Complete Freund’s Adjuvant (CFA)-Induced Arthritis by Regulating Inflammation and Oxidative Stress

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