Cytotoxicity of Zinc Oxide Nanoparticles on Antioxidant Enzyme Activities and mRNA Expression in the Cocultured C2C12 and 3T3-L1 Cells

Pandurangan, Muthuraman; Veerappan, Muthuviveganandavel; Kim, Doo Hwan

doi:10.1007/s12010-014-1351-y

Cited by 45 publications

(29 citation statements)

References 31 publications

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“…This was based on the spectrophotometric method of Pandurangan et al (7). Malondialdehyde (MDA) was measured by determining the thiobarbituric acid reactive species.…”

Section: Methodsmentioning

confidence: 99%

“…This was based on the spectrophotometric method of Pandurangan et al (7). The yellow product color was measured at 405 nm (Cary 100 UV-Vis spectrophotometer; Agilent Technologies, Inc.).…”

Section: Methodsmentioning

confidence: 99%

“…SOD, catalase and lactate dehydrogenase (LDH) enzyme activities were determined using the antioxidant enzyme assay kit method (Abcam) which was based on the method of Pandurangan et al (7). …”

Section: Methodsmentioning

confidence: 99%

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Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats

et al. 2016

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The present study evaluated the protective effect of selenium against cisplatin-induced nasopharyngeal cancer in the cardiac tissue of adult rats. Male Wistar rats were divided into 4 groups of 6 rats each. Different combinations of selenium and cisplatin were administered for 45 days, following which the animals were sacrificed and the nasopharyngeal tissue was surgically removed. Lipid peroxidation, reduced glutathione, and the antioxidant enzymes superoxide dismutase (SOD), catalase and lactate dehydrogenase (LDH) were measured. Apoptotic-related gene (p53, bax and caspase 3) mRNA expression was determined using quantitative polymerase chain reaction. Caspase 3 activity was also measured in all the groups. The results demonstrated that selenium significantly reduced the levels of malondialdehyde. The levels of glutathione, SOD, LDH and catalase significantly increased following selenium treatment. Relative mRNA expression (p53, bax and caspase 3) was significantly reduced in the cisplatin-treated rats, but it significantly increased following selenium treatment. The anticancer activity of selenium was also investigated in HK1cells. Fluorescence and confocal microscopy were used to analyze apoptosis and reactive oxygen species. The protective effect of selenium was also evident through caspase 3 activity, which significantly increased following selenium treatment. Taken together, these results indicate that selenium may be beneficial against cisplatin-induced nasopharyngeal cancer.

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“…This was based on the spectrophotometric method of Pandurangan et al (7). Malondialdehyde (MDA) was measured by determining the thiobarbituric acid reactive species.…”

Section: Methodsmentioning

confidence: 99%

“…This was based on the spectrophotometric method of Pandurangan et al (7). The yellow product color was measured at 405 nm (Cary 100 UV-Vis spectrophotometer; Agilent Technologies, Inc.).…”

Section: Methodsmentioning

confidence: 99%

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Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats

et al. 2016

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“…[26] ZnO NPs have demonstrated their preferential ability to kill high proliferative cells, like cancer cells, versus normal cells. [19,27] In addition, the oxidative stress properties of ZnO NPs have been also tested; [28] ZnO NPs can be sequestered by autophagy and this internalization could generate reactive oxygen species (ROS) intracellularly. [29] The effect of surfaces containing ZnO in cell behaviour has been investigated previously.…”

Section: Introductionmentioning

confidence: 99%

PLLA/ZnO nanocomposites: Dynamic surfaces to harness cell differentiation

Trujillo

Lizundia

Vilas

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Graphical abstract2 Highlights  Biodegradable poly(L-lactide) matrix loaded with ZnO nanorods. Dynamic presentation of nanorods triggered by PLLA degradation. Changes in surface properties as a function of time control cell behaviour. Nanorods exposure promotes cell differentiation.3 Keywords: PLLA, ZnO nanoparticle, C2C12 myoblast, nanocomposite, cell differentiation AbstractThis work investigates the effect of the sequential availability of ZnO nanoparticles, (nanorods of ~ 20 nm) loaded within a degradable poly(lactic acid) (PLLA) matrix, in cell differentiation. The system constitutes a dynamic surface, in which nanoparticles are exposed as the polymer matrix degrades. ZnO nanoparticles were loaded into PLLA and the system was measured at different time points to characterise the time evolution of the physicochemical properties, including wettability and thermal properties. The micro and nanostructure were also investigated using AFM, SEM and TEM images. Cellular experiments with C2C12 myoblasts show that cell differentiation was significantly enhanced on ZnO nanoparticles -loaded PLLA, as the polymer degrades and the availability of nanoparticles become more apparent, whereas the release of zinc within the culture medium was negligible. Our results suggest PLLA/ZnO nanocomposites can be used as a dynamic system where nanoparticles are exposed during degradation, activating the material surface and driving cell differentiation.4

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“…ZnO-NPs get absorbed systemically in the liver, adipose, and pancreas, which can elevate the level of zinc in the body [21]. The stimulation of oxidative stress is the vital part of the cytotoxicity of ZnO-NPs [22]. The major challenge in the current study is to deal with the size, density, and surface property variation of the nanomaterial because in the case of in vitro and in vivo experimental settings, these parameters were either poorly determined or with very broad range [23].…”

Section: Introductionmentioning

confidence: 99%

Elucidation the Toxicity Mechanism of Zinc Oxide Nanoparticle Using Molecular Docking Approach With Proteins

Singh

Dhasmana

Rahman

2018

Asian J Pharm Clin Res

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Objective: At present, toxicological tests are resource-intensive, time-consuming and require a large pool of animal models for toxicity assessment. To speed up the toxicity evaluation and to reduce animal suffering during toxicity assessment, the use of alternative methods including computational models is in high demand. The computational toxicity prediction methods are very helpful for the regulatory bodies to quickly assess the health impact of nanomaterial materials. In the present work, we have examined the mechanism of zinc oxide nanoparticle (ZnO-NP)-proteins interaction and their effect of surface chemistries of ZnO-NP on the bioactive conformation of chemokines and other cytological proteins using in silico molecular docking approaches.Methods: Molecular docking study was conducted using AutoDock 4.0 version and the visualization result using Discover Studio 4.0. Results:In the present study, we observed that ZnO-NP has high binding affinity with the mitogen-activated protein kinases (P-38), nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kB) proteins, and matrix metallopeptidase-9 with docking energies −8.81, −7.64, and −7.27 Kcal/Mol, respectively, involving with hydrogen, metal acceptor, and electrostatic interaction. The top interacting amino acid residues with ZnO-NP are GLY, PHE, ARG, ASP, GLN, and ASN. Conclusion:Thus, based on the molecular docking studies, we determine that the ZnO-NP is strongly interacting with the chemokines and other cytological proteins thus responsible for blocking of the activation stimuli for these proteins to initiate the biological signals for the proper functioning. We have also extracted the information of interaction pattern of ZnO-NP with the surface-enriched amino acid residues of chemokine and cytological proteins using molecular docking approach.

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Cytotoxicity of Zinc Oxide Nanoparticles on Antioxidant Enzyme Activities and mRNA Expression in the Cocultured C2C12 and 3T3-L1 Cells

Cited by 45 publications

References 31 publications

Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats

Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats

PLLA/ZnO nanocomposites: Dynamic surfaces to harness cell differentiation

Elucidation the Toxicity Mechanism of Zinc Oxide Nanoparticle Using Molecular Docking Approach With Proteins

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