Cytotoxicity of Vincristine on the 5637 Cell Line Is Enhanced by Combination with Conferone

Neshati, Vajiheh; Matin, Maryam Moghaddam; Iranshahi, Mehrdad; Bahrami, Ahmad Reza; Behravan, Javad; Mollazadeh, Samaneh; Rassouli, Fatemeh Behnam

doi:10.1515/znc-2009-5-602

Cited by 19 publications

(7 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This activity was shown to be due to the potent inhibition of P-glycoprotein by this phytochemical and consequent reversal of multidrug resistance [15]. The same effect of conferone has also been reported for vincristine against 5637 cell line [16]. However, in both of these studies, treatment of cell lines with conferone alone did not result in any considerable cytotoxic activity.…”

Section: Resultsmentioning

confidence: 74%

Cytotoxic activities of phytochemicals from Ferula species

2013

Self Cite

View full text Add to dashboard Cite

BackgroundFerula species are reputed in folk medicine for the treatment of a variety of disorders. There have been sporadic reports on the chemopreventive and chemosensitizing activities of some terpenoid coumarin derivatives from the genus Ferula. The present study investigated the cytotoxic activity of 11 phytochemicals (conferone, farnesiferol A, acantrifoside E, mogoltadone, diversin, galbanic acid, herniarin, 7-isopentenyloxycoumarin, umbelliprenin, stylosin and tschimgine) from Ferula species together with a newly synthesized prenylated derivative of curcumin (gercumin II).MethodsCytotoxic activity of phytochemicals was evaluated against ovarian carcinoma (CH1), lung cancer (A549) and melanoma (SK-MEL-28) cell lines using MTT assay.Results and conclusionOverall, moderate cytotoxic activity was observed from the tested compounds with IC50 values in the micromolar range. The highest activity against CH1 and A549 lines was from conferone while stylosin and tschimgine were the most potent compounds against SK-MEL-28 line. In conclusion, the findings of the present investigation did not support a potent cytotoxic activity of the tested phytochemicals against CH1, A549 and SK-MEL-28 cell lines. With respect to previous reports, the beneficial impact of these phytochemicals in cancer therapy may be more attributable to their chemopreventive or chemosensitizing activity rather than direct cytotoxic effects.

show abstract

Section: Resultsmentioning

confidence: 74%

Cytotoxic activities of phytochemicals from Ferula species

2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…After the plate was transferred into an incubator with 5% CO 2 content at 37 • C for 30 min, a microplate reader was used to read the optical density at 570 nm. The absorbance value of the cell culture control group was considered 100% viable; then, using that value, the formula below was used to calculate the percent viability of other cell groups: cell viability (%): mean sample absorbance/mean control absorbance × 100 [19,20].…”

Section: Cell Viability Testmentioning

confidence: 99%

Lactic Acid Bacteria-Derived Exopolysaccharides Mitigate the Oxidative Response via the NRF2-KEAP1 Pathway in PC12 Cells

Şirin

2023

CIMB

View full text Add to dashboard Cite

Parabiotics, including L-EPSs, have been administered to patients with neurodegenerative disorders. However, the antioxidant properties of L-EPSs against H2O2-induced oxidative stress in PC12 cells have not been studied. Herein, we aimed to investigate the antioxidant properties of the L-EPSs, their plausible targets, and their mechanism of action. We first determined the amount of L-EPSs in Lactobacillus delbrueckii ssp. bulgaricus B3 and Lactiplantibacillus plantarum GD2 using spectrophotometry. Afterwards, we studied their effects on TDH, TOS/TAS, antioxidant enzyme activities, and intracellular ROS level. Finally, we used qRT-PCR and ELISA to determine the effects of L-EPSs on the NRF2-KEAP1 pathway. According to our results, the L-EPS groups exhibited significantly higher total thiol activity, native thiol activity, disulfide activity, TAS levels, antioxidant enzyme levels, and gene expression levels (GCLC, HO-1, NRF2, and NQO1) than did the H2O2 group. Additionally, the L-EPS groups caused significant reductions in TOS levels and KEAP1 gene expression levels compared with those in the H2O2 group. Our results indicate that H2O2-induced oxidative stress was modified by L-EPSs. Thus, we revealed that L-EPSs, which regulate H2O2-induced oxidative stress, could have applications in the field of neurochemistry.

show abstract

“…Sesquiterpene coumarins have also been shown to enhance the cytotoxicity of chemotherapeutic agents. For example, mogoltacin, conferone and feselol, sesquiterpene coumarins from Ferula badrakema, can significantly enhance the cytotoxicity of vincristine in transitional cell carcinoma cells [143][144][145]. Other alternative approaches to target P-glycoprotein-mediated drug resistance could involve the development of agents to interfere with any one of the regulatory steps in expression of P-glycoprotein: transcription, mRNA turnover, translation, protein processing, and turnover [53,146].…”

Section: Elimination Therapymentioning

confidence: 99%

Cancer stem cells and cancer therapy

2011

Self Cite

View full text Add to dashboard Cite

Cancer stem cells (CSCs) are a subpopulation of tumour cells that possess the stem cell properties of self-renewal and differentiation. Stem cells might be the target cells responsible for malignant transformation, and tumour formation may be a disorder of stem cell self-renewal pathway. Epigenetic alterations and mutations of genes involved in signal transmissions may promote the formation of CSCs. These cells have been identified in many solid tumours including breast, brain, lung, prostate, testis, ovary, colon, skin, liver, and also in acute myeloid leukaemia. The CSC theory clarifies not only the issue of tumour initiation, development, metastasis and relapse, but also the ineffectiveness of conventional cancer therapies. Treatments directed against the bulk of the cancer cells may produce striking responses but they are unlikely to result in long-term remissions if the rare CSCs are not targeted. In this review, we consider the properties of CSCs and possible strategies for controlling the viability and tumourigenecity of these cells, including therapeutic models for selective elimination of CSCs and induction of their proper differentiation.

show abstract

Cytotoxicity of Vincristine on the 5637 Cell Line Is Enhanced by Combination with Conferone

Cited by 19 publications

References 15 publications

Cytotoxic activities of phytochemicals from Ferula species

Cytotoxic activities of phytochemicals from Ferula species

Lactic Acid Bacteria-Derived Exopolysaccharides Mitigate the Oxidative Response via the NRF2-KEAP1 Pathway in PC12 Cells

Cancer stem cells and cancer therapy

Contact Info

Product

Resources

About