Cytotoxicity of Various Fractions of Compounds Extracted from Yoyo Bitters on Human Cervical Cancer Cells

Onyeaghala, Augustine Anayochukwu; Omotosho, I. O.; Shivashankara, Arnadi Ramachandrayya

doi:10.9734/ejmp/2015/15913

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…The fractionation process was carried out according to the method described by Onyeaghala et al [ 28 ] with slight modifications. Briefly, 2 × 200 mL of the bitters was exhaustively extracted using n-hexane.…”

Section: Methodsmentioning

confidence: 99%

Five traditional Nigerian Polyherbal remedies protect against high fructose fed, Streptozotocin-induced type 2 diabetes in male Wistar rats

Kale

Akinpelu

Bakare

et al. 2018

BMC Complement Altern Med

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BackgroundThis present study sought to assess the modulatory effects of five Nigerian traditional polyherbal in high fructose-fed, streptozotocin-induced (HF-STZ) Type 2 diabetes (T2D) in rats. T2D was achieved via fructose feeding (20%W/V) ad libitum for 2 weeks and streptozotocin (STZ, 40 mg/kg) (15th Day) intraperitoneally.MethodsSeventy-two hours after STZ injection, fourty-eight diabetic rats were divided into eight of 6 rats/group: Diabetic normal untreated, glibenclamide (GBLI, 0.07 mL/kg) or yoyo (YB, 0.43), ruzu (RB, 0.08), fajik (FJB, 0.20), oroki (OB, 0.16), and fidson (FB, 0.43)/ mL/kg bitters respectively. Controls normal and diabetic untreated groups received intragastric carboxylmethylcellulose (CMC, 1 mL/kg) for eleven days.ResultsT2D was characterized in rats by an increased (p < 0.001–0.05) blood glucose levels (BGL), total cholesterol, triglycerides, low-density lipoprotein and alanine aminotransferase compared with control CMC group. Similarly, hepatic and pancreatic malondialdehyde (MDA) were increased by 180 and 97% respectively. Polyherbal treatments demonstrated efficacies on BGL as follow: YB (55.6%, 160.7 mg/dL); RB (59.7%, 145.2 mg/dL); FJB (59.8%, 243.4 mg/dL); OB (60.8%, 194.5 mg/dL) and FB (61.3%, 203.3 mg/dL) respectively by day 11 (versus GBLI, 65.1%) compared with control untreated diabetic rats. Also, elevated TC, LDL cholesterol, ALT were lowered (p < 0.05) by YB, FJB, and FB respectively in rats. YB, FJB, and OB lowered MDA levels in treated rats. Further, YB, RB, FJB and FB restored changes in liver, and pancreas histopathology. Predominant non-polar bioactive include oleic, hexadecanoic, octadecanoic among others following gas chromatography-mass spectrophotometry analyses.ConclusionOverall, these present results demonstrate anti-hyperglycemic potentials, although with cautions, of some polyherbal in T2D rats, which may, in part, be antioxidants mediated.

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Section: Methodsmentioning

confidence: 99%

Five traditional Nigerian Polyherbal remedies protect against high fructose fed, Streptozotocin-induced type 2 diabetes in male Wistar rats

Kale

Akinpelu

Bakare

et al. 2018

BMC Complement Altern Med

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“…This variation in pH could be due to batch to batch differences mostly caused by poor adherence to strict quality control measures. A study [14] on chemical 0 and alkaloids has been known to be responsible for the bitter taste observed in many foods and herbs. This can probably account for the bitterness of Yoyo bitters.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Yoyo Bitters on the Dissolution of Metformin Tablets

Igbokwe

Muazu

2017

JAPLR

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Background: Yoyo bitters is an oral poly herbal liquid preparation popularly marketed and used in Nigeria. Its use is rapidly increasing among patients with chronic diseases such as diabetes and hypertension. This is because it has been claimed to regulate blood pressure, control body weight, and facilitate digestion among others. Metformin is an oral anti diabetic agent. Both the incidences of Diabetes and use of Yoyo bitters are increasing hence setting the stage for possible co-administration of these two agents. This also increases the potential for Herb-drug interaction. The aim of the study is to investigate the effect of Yoyo bitters on the dissolution of metformin tablets.Methods: Physicochemical tests (Weight uniformity, Crushing strength, Friability and Assay) were carried out on two different brands of metformin tablets and physical properties of the Yoyo bitters were also determined. The in-vitro dissolution of metformin tablets with and without Yoyo bitters in dissolution media was then carried out at pH 1.2, 4.5 and 6.8 using United States Pharmacopoeia (USP) apparatus 1 (basket) at 100rpm with 900ml of the dissolution medium (sink condition) maintained at a steady temperature of 37±0.5ºC. Two different brands metformin 500mg immediate release tablets were used and the analysis was done using UVSpectrophotometer at a wavelength of 232nm. Results:The results obtained show that at pH 1.2, the percentage amount of metformin released decreased significantly in the presence of Yoyo bitters for both brands (p=0.002 and p=0.013 respectively). At pH 4.5 there was no significant difference in metformin released for the Diabetmin ® (p=0.196) and a significant difference was observed with Sandoz ® (p=0.045). At pH 6.8, a significant decrease in percentage metformin released in the presence of Yoyo bitters was observed for both brands (p=0.011 and p=0.044 respectively). Conclusion:Yoyo bitters decreased the dissolution of metformin tablets at pH 1.2, 4.5 and 6.8. Due to therapeutic failure, toxicity etc caused by decreased dissolution, concomitant use of metformin and Yoyo bitters should be discouraged.

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“…In addition, the extract of abiu shoot leaves also contained a 1-fluoro dodecane compound (Table 1). The 1-fluoro dodecane compound functioned as an anti-cervical cancer [24]. The 1-fluoro dodecane compound has also functioned as anti-bacterial, anti-tumor, anti-cancer, antioxidant, anti-microbial, anti-HIV, and anti-viral [25].…”

Section: Abiu Leavesmentioning

confidence: 99%

Identification of Volatile Compounds in Several Parts of Abiu (Pouteria Caimito)

Arif

Susanto

Matra

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Abiu (Pouteria caimito) is one of the exotic plants native to the Amazon and has been introduced in Indonesia. Abiu has contained beneficial compounds for health. Therefore, the hexane extract from several parts of abiu were identified for its volatile compound content. The study used 6 (six) parts of abiu plant, i.e. shoot leaves, mature leaves, unripe fruit, ripe fruit pulp, ripe fruit peel and abiu seed. The contents of volatile compounds were determined using gas chromatography mass spectrometry (GCMS). The results showed that the volatile compounds in abiu leaves tended to be dominated by compounds that acted for plant protection (capsaicin and 1-fluoro dodecane). The volatile compounds in unripe fruit consisted 5-hydroxy-4-methyl-6-hepten-3-one, methylcyclopentane, 2-hexenal and 3-hexanol compounds. In ripe fruit pulp, it were identified ketones, aldehydes (hexanal and 2-hexenal), and alcohol (3-hexanol) were identified. These compounds are related to the aroma in the ripening process of abiu fruit. The volatile compounds identified in abiu fruit peel were aldehyde compounds (2-hexenal and 2-propenal) and capsaicin, which are also related to aroma and fruit protection.

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Cytotoxicity of Various Fractions of Compounds Extracted from Yoyo Bitters on Human Cervical Cancer Cells

Cited by 5 publications

References 17 publications

Five traditional Nigerian Polyherbal remedies protect against high fructose fed, Streptozotocin-induced type 2 diabetes in male Wistar rats

Five traditional Nigerian Polyherbal remedies protect against high fructose fed, Streptozotocin-induced type 2 diabetes in male Wistar rats

The Effect of Yoyo Bitters on the Dissolution of Metformin Tablets

Identification of Volatile Compounds in Several Parts of Abiu (Pouteria Caimito)

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