Cytotoxicity of titania nanoparticles towards waste water isolate Exiguobacterium acetylicum under UVA, visible light and dark conditions

Mathur, Asha; Raghavan, Archanaa; Chaudhury, Pallavi; Johnson, Jerald B.; Roy, Rajdeep; Kumari, Jaya; Chaudhuri, Gouri; Chandrasekaran, N.; Suraishkumar, G. K.; Mukherjee, Amitava

doi:10.1016/j.jece.2015.06.026

Cited by 10 publications

(3 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous studies showed no significant chemical dissolution of TiO 2 -NPs in treated waste water medium, under dark and UVA conditions of exposure. Furthermore, the presence of Ti 4+ ions in the procured waste water, did not affect the chemical dissolution of TiO 2 -NPs [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

“…Our previous study [ 20 ] with TiO 2 -NPs mainly focused on the types of ROS generation under UVA, visible light and dark conditions with single dominant waste water bacterial isolate. The present work rather deals with the comparative toxic effects of TiO 2 -NPs on five dominant individual waste water isolates and their consortium in test medium.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Decreased Phototoxic Effects of TiO₂ Nanoparticles in Consortium of Bacterial Isolates from Domestic Waste Water

et al. 2015

Self Cite

View full text Add to dashboard Cite

This study is aimed to explore the toxicity of TiO2 nanoparticles at low concentrations (0.25, 0.50 & 1.00 μg/ml); on five bacterial isolates and their consortium in waste water medium both in dark and UVA conditions. To critically examine the toxic effects of nanoparticles and the response mechanism(s) offered by microbes, several aspects were monitored viz. cell viability, ROS generation, SOD activity, membrane permeability, EPS release and biofilm formation. A dose and time dependent loss in viability was observed for treated isolates and the consortium. At the highest dose, after 24h, oxidative stress was examined which conclusively showed more ROS generation & cell permeability and less SOD activity in single isolates as compared to the consortium. As a defense mechanism, EPS release was enhanced in case of the consortium against the single isolates, and was observed to be dose dependent. Similar results were noticed for biofilm formation, which substantially increased at highest dose of nanoparticle exposure. Concluding, the consortium showed more resistance against the toxic effects of the TiO2 nanoparticles compared to the individual isolates.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Decreased Phototoxic Effects of TiO₂ Nanoparticles in Consortium of Bacterial Isolates from Domestic Waste Water

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Remediation of hazardous substances is attributed to hydroxyl radical (•OH), which exhibits reactivity toward organics (Kim et al, 2012;Aljuboury et al, 2015a), which has a high oxidation potential (estimated to be 2.8 V) relative to other oxidants (Al-Rasheed, 2005). The generation of hydroxyl and superoxide radicals was more in the TiO2 exposed to UV condition as compared to the TiO2 exposed to visible light and dark condition (Mathur et al, 2015).…”

Section: Introductionmentioning

confidence: 83%

Inorganic carbon removal from refinery wastewater by using TiO2/ZnO/Fenton photocatalyst

2018

Global NEST Journal

View full text Add to dashboard Cite

The aim of this study is to investigate the performance of the solar photocatalyst of TiO2/ZnO/Fenton process to treat the refinery wastewater and remove inorganic carbon (IC) which potentially toxic to human, aquatic and microorganism life. Central composite design with response surface methodology was used to evaluate the relationships between operating variables for TiO2 dosage, ZnO dosage, Fe2+ dosage, H2O2 dosage, and pH to identify the optimum operating conditions. Quadratic models for inorganic carbon (IC) removal and residual iron prove to be significant with low probabilities (<0.0001). The (IC) removal rates and residual iron correspond well with the predicted models. The maximum removal rate for IC and residual iron was 92.3% and 0.013, respectively at optimum operational conditions of a TiO2 dosage (0.3 g/l), ZnO dosage (0.58 g/l), Fe2+ dosage (0.02 g/l), H2O2 dosage (2.7 g/l), and pH (7). The treatment process achieved higher degradation efficiencies for IC and reduced the treatment time comparing with other related processes.

show abstract

Mechanism of the oxidative stress‐mediated increase in lipid accumulation by the bacterium, R. opacus PD630: Experimental analysis and genome‐scale metabolic modeling

Sundararaghavan

Mukherjee

Sahoo

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

Appropriate species of oleaginous bacteria, with their high growth rates and lipid accumulation capabilities, can be good contenders for industrial triacylglycerol (TAG) production, compared to microalgae. Further, oxidative stress (OS) can be used to significantly increase TAG yields in oleaginous microbes, but the mechanism is unexplored. In a first, this study explored the mechanism behind OS‐mediated increase in TAG accumulation by the bacterium, Rhodococccus opacus PD630, through experimental analysis and metabolic modelling. Two mechanisms that could increase acetyl‐CoA (TAG‐precursor) levels were hypothesized based on literature information. One was OS‐mediated inactivation of the aconitase (TCA cycle), and another was the inactivation of the triosephosphate isomerase (TPI; glycolysis). The results negated the involvement of aconitase in increased acetyl‐CoA levels. Analysis of the metabolic model showed that inactivation of TPI, re‐routed the flux through the pentose phosphate pathway (PPP), supplying both NADPH and acetyl‐CoA for TAG synthesis. Additionally, inactivation of TPI increased TAG flux by 143%, whereas, inactivating both TPI and aconitase, increased it by 152%. We present experimental evidence for OS‐mediated decrease in TPI activity and increase in activity of glucose‐6‐phosphate dehydrogenase (PPP enzyme). The findings indicate that increased flux through PPP can be explored to improve TAG accumulation on a large‐scale.

show abstract

Cytotoxicity of titania nanoparticles towards waste water isolate Exiguobacterium acetylicum under UVA, visible light and dark conditions

Cited by 10 publications

References 45 publications

Decreased Phototoxic Effects of TiO₂ Nanoparticles in Consortium of Bacterial Isolates from Domestic Waste Water

Decreased Phototoxic Effects of TiO₂ Nanoparticles in Consortium of Bacterial Isolates from Domestic Waste Water

Inorganic carbon removal from refinery wastewater by using TiO2/ZnO/Fenton photocatalyst

Mechanism of the oxidative stress‐mediated increase in lipid accumulation by the bacterium, R. opacus PD630: Experimental analysis and genome‐scale metabolic modeling

Contact Info

Product

Resources

About