Cytotoxicity of the methanol extracts of Elephantopus mollis, Kalanchoe crenata and 4 other Cameroonian medicinal plants towards human carcinoma cells

Kuete, Victor; Fokou, Fabrice W.; Karaosmanoğlu, Oğuzhan; Beng, Véronique Penlap; Sivas, Hülya

doi:10.1186/s12906-017-1793-1

Cited by 45 publications

(20 citation statements)

References 41 publications

(37 reference statements)

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“…Cancer in low- and middle-income countries caused about 70% of death ( Kuete et al, 2017 ). About 15–25% of all females cancer cases and deaths were caused by breast cancer while universally the second major cause of cancer death in males was liver cancer ( Torre et al, 2015 ).…”

Section: Resultsmentioning

confidence: 99%

Phytochemical profile, antioxidant and cytotoxic potential of Parkinsonia aculeata L. growing in Saudi Arabia

Abdelaziz

Yousef

Alqahtani

et al. 2020

Saudi Pharmaceutical Journal

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Parkinsonia aculeata L. growing in Saudi Arabia was investigated for its phytochemical profile, antioxidant, and cytotoxic properties. UPLC-ESI-MS/MS was employed as a powerful technique for the characterization of secondary metabolites from a hydroalcoholic extract, dichloromethane, and ethyl acetate fractions of P. aculeata L. aerial parts. Sixty-nine compounds (flavonoids, anthocyanins, phenolics and fatty acids) were detected and characterized; flavonoids were the abundant components in the analyzed samples. The dichloromethane fraction was rich in phenolics as vanillic acid hexoside, flavonols as 3,7-dimthylquercetin, and flavones as 3′-hydroxymelanettin. However, the ethyl acetate fraction was rich in flavonoid- C -glycosides as luteolin-8- C -β-D-glucoside (orientin) and apigenin-8- C -glucoside (vitexin), flavonoid- O , C -diglycosides such as luteolin 7- O -[6′'-dihydrogalloyl]-glucosyl-8- C -pentosyl-(1 → 2)-glucoside and 2′'- O -rhamnosyl isoorientin. These compounds were identified for the first time in dichloromethane and ethyl acetate fractions of Saudi P. aculeata L. Additionally, all the samples were assessed for antioxidant activity using DPPH radical scavenging method and for cytotoxic activity through MTT assay. Accordingly, the most active fraction was the ethyl acetate which showed the highest antioxidant activity (SC 50 = 57.4 ± 1.2 μg/mL) compared with the positive control, ascorbic acid (SC 50 = 12.4 ± 0.5 μg/mL) and moderate cytotoxicity against HepG-2 (hepatocellular carcinoma) and MCF-7 (breast carcinoma) cell lines with IC 50 = 56.9 ± 3.1 and 95.8 ± 3.8 μg/mL, respectively compared with cisplatin (IC 50 = 3.67 ± 0.22 and 5.71 ± 0.57 μg/mL, respectively for both cell lines). The antioxidant and cytotoxic activities may be attributed to the presence of high percentage of phenolic compounds and hydroxylated flavonoids detected in ethyl acetate fraction using UPLS-ESI-MS/MS.

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Section: Resultsmentioning

confidence: 99%

Phytochemical profile, antioxidant and cytotoxic potential of Parkinsonia aculeata L. growing in Saudi Arabia

Abdelaziz

Yousef

Alqahtani

et al. 2020

Saudi Pharmaceutical Journal

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“…The IC 50 value of doxorubicin, which was evaluated by NRU experiments in our laboratory in MCF-7 cells, was previously reported as 0.19 mg/mL. 24 Based on these promising results for the selective cytotoxicity on the hepatocellular carcinoma cells, it was decided to implement further experiments to figure out the ACES-induced possible death mechanism.…”

Section: Aces Exhibits Selective Toxicity To Huh-7 Cellsmentioning

confidence: 99%

P38-β/SAPK-inhibiting and apoptosis-inducing activities of (E)-4-chloro-2-((3-ethoxy-2-hydroxybenzylidene) amino)phenol

Karaosmanoğlu

2020

Hum Exp Toxicol

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The present study has three purposes; first evaluating cytotoxicity of (E)-4-chloro-2-((3-ethoxy-2-hydroxybenzylidene)amino)phenol (ACES), second deciphering ACES-mediated cellular death mechanism, and third estimating ACES-mediated alterations in the expressions of mitogen-activated protein kinase (MAPK) pathway-related genes. Neutral red uptake assay, cell cycle analysis, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) measurements, caspase 3/7 and 9 activations, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were implemented. IC50 values of ACES-treated five cells were around 4–6 µg/mL. However, Caco-2 and Huh-7 cells were found to be twofold resistant and fivefold sensitive with IC50 values of 11 µg/mL and 0.93 µg/mL, respectively. In this study, it was initially reported that ACES exhibits selective cytotoxicity to Huh-7 cells. In addition, ACES induced apoptosis by nuclear fragmentation, MMP disruption, and intracellular ROS elevation in MCF-7 cells. qRT-PCR experiment indicated the expressions of 30 genes including ATF2, CREB1, MYC, NFATC4 (NFAT3), CCNA1, CCNB1, CCND2, CDK2, CDKN1A (p21CIP1), CDKN1C (p57KIP2), CDKN2A (p16INK4a), CDKN2B (p15INK4b), DLK1, NRAS, CDC42, PAK1, MAP4K1 (HPK1), MAP3K3 (MEKK3), MAP2K3 (MEK3), MAP2K6 (MEK6), MOS, MAPK1 (ERK2), MAPK8 (JNK1), MAPK10 (JNK3), MAPK11 (p38-β), LAMTOR3 (MP1), MAPK8IP2 (JIP-1), PRDX6 (AOP2), COL1A1, and HSPA5 (Grp78) were downregulated at least 1.5-fold. Moreover, ACES effectively inhibited expressions of genes that code for elements of p38-β/stress-activated protein kinase (SAPK) pathway. ACES has the potential to be used for the reversal of trastuzumab resistance in breast cancer patients by inhibiting p38/SAPK pathway in MCF-7 cells. Therefore, with the selective cytotoxic, apoptosis-inducing, and p38-β/SAPK-inhibiting activities, ACES can be utilized for developing a novel anticancer drug.

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“…8 There have been many studies on the value of EM in the treatment of non-communicable diseases. 9,10 However, research on the botanical and bioactive characteristics with respect to the soil conditions of EM in the south of Vietnam is still being very limited. In addition, the hepatoprotective effects of EM have only been verified in vitro.…”

Section: Introductionmentioning

confidence: 99%

Acute Toxicity and Liver Protective Effects of Elephantopus mollis H.B.K

2021

TJNPR

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Elephantopus mollis (EM) is a wild species and is commonly used in folk medicine as remedy for various ailments. Some uses of this plant has been documented in the scientific literature. However, studies on the medicinal properties of this specie remain rarely published and the specie has not been recorded in the Vietnamese pharmacopeia. The present study therefore assessed the acute toxicity and liver-protective effects of Elephantopus mollis (EM). The acute toxicity of the aqueous extract of EM in mice was assessed following the method stipulated by the Organization for Economic Cooperation and Development (OECD), while the hepatoprotective effect was assessed following previously reported method. Whole plant extract of EM was not toxic to the tested mice (at a dose of 9,000 mg/kg) and showed good liver protection. The plant extract had the ability to reduce the degradation of liver cells by reducing the amount of liver enzymes. At a dose of 400 mg/kg EM, the reduction efficiency of AST and ALT in tested mice were 71.43 ± 11.36% and 63.82 ± 19.43%, respectively, when compared with the negative control group after 2 weeks of treatment. Mice liver morphology and histopathology treated with EM extract showed a decent improvement. The liver cells size of mice treated with EM extract decreased significantly (28.02 ± 6.33 µm) compared to the mice in the negative control group (34.82 ± 6.36 µm). The results suggest the potential effect of this extract as a hepatoprotective agent towards liver damage without any oral acute toxicity effect.

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Cytotoxicity of the methanol extracts of Elephantopus mollis, Kalanchoe crenata and 4 other Cameroonian medicinal plants towards human carcinoma cells

Cited by 45 publications

References 41 publications

Phytochemical profile, antioxidant and cytotoxic potential of Parkinsonia aculeata L. growing in Saudi Arabia

Phytochemical profile, antioxidant and cytotoxic potential of Parkinsonia aculeata L. growing in Saudi Arabia

P38-β/SAPK-inhibiting and apoptosis-inducing activities of (E)-4-chloro-2-((3-ethoxy-2-hydroxybenzylidene) amino)phenol

Acute Toxicity and Liver Protective Effects of Elephantopus mollis H.B.K

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