Cytotoxicity of prodigiosin and benznidazole on V79 cells

Melo, Patrı́cia da Silva; Durán, Nélson; Haun, Marcela

doi:10.1016/s0378-4274(00)00226-5

Cited by 29 publications

(13 citation statements)

References 33 publications

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“…Prodigiosin induces apoptotic phenomena and cell death in cancer cell lines [18]. In addition, it has been reported to have a strong inhibitory effect on a V79 fibroblast cell line [19]. However, other studies failed to demonstrate cytotoxicity against normal cells as compared with tumor cells lines [20].…”

Section: Discussionmentioning

confidence: 99%

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Mitochondrial dysfunction in Trypanosoma cruzi: the role of Serratia marcescens prodigiosin in the alternative treatment of Chagas disease

et al. 2011

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BackgroundChagas disease is a health threat for many people, mostly those living in Latin America. One of the most important problems in treatment is the limitation of existing drugs. Prodigiosin, produced by Serratia marcescens (Rhodnius prolixus endosymbiont), belongs to the red-pigmented bacterial prodiginine family, which displays numerous biological activities, including antibacterial, antifungal, antiprotozoal, antimalarial, immunosuppressive, and anticancer properties. Here we describe its effects on Trypanosoma cruzi mitochondria belonging to Tc I and Tc II.ResultsParasites exposed to prodigiosin altered the mitochondrial function and oxidative phosphorylation could not have a normal course, probably by inhibition of complex III. Prodigiosin did not produce cytotoxic effects in lymphocytes and Vero cells and has better effects than benznidazole. Our data suggest that the action of prodigiosin on the parasites is mediated by mitochondrial structural and functional disruptions that could lead the parasites to an apoptotic-like cell death process.ConclusionsHere, we propose a potentially useful trypanocidal agent derived from knowledge of an important aspect of the natural life cycle of the parasite: the vector-parasite interaction. Our results indicate that prodigiosin could be a good candidate for the treatment of Chagas disease.

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Section: Discussionmentioning

confidence: 99%

“…However, other studies failed to demonstrate cytotoxicity against normal cells as compared with tumor cells lines [20]. In addition, prodigiosin derivatives show marked activity against Plasmodium falciparum [21], and strong activity against T. cruzi has also been reported [19]. …”

Section: Discussionmentioning

confidence: 99%

Mitochondrial dysfunction in Trypanosoma cruzi: the role of Serratia marcescens prodigiosin in the alternative treatment of Chagas disease

et al. 2011

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“…Prodigiosin, a bacterial metabolite, is a tripyrrole nitrogen ring pigment synthesized by Serratia marcescens (Williams, 1973;Kavitha et al, 2010). This red pigment is a promising drug owing to its antimicrobial and immunomodulatory activities (Lee et al, 1995;Da Silva Melo et al, 2000). It is produced by Serratia marcescens following a bifurcated biosynthesis pathway in which the separately obtained mono and bipyrrole precursors are coupled to form the linear tripyrrole red pigment (Boger and Patel, 1988) during the stationary phase of bacterial growth (Rokem and Weitzman, 1987).…”

Section: Introductionmentioning

confidence: 99%

In silico and In vitro cytotoxic effect of prodigiosin-conjugated silver nanoparticles on liver cancer cells (HepG2)

El-Batal¹,

El-Hendawy²,

Faraag³

2017

bta

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This study demonstrates a novel approach for the synthesis of silver nanoparticles (AgNPs) against human liver cancer cell line (HepG2) using prodigiosin pigment isolated from Serratia marcescens. It further investigates the influence of various parameters such as initial pH, temperature, silver nitrate (AgNO 3) concentration, and prodigiosin concentration on stability and optical properties of synthesized prodigiosin AgNPs. Highly stable, spherical prodigiosin-conjugated AgNPs were synthesized with a mean diameter of 9.98 nm using a rapid one-step method. The cytotoxic activity investigated in the present study indicated that prodigiosin and prodigiosin-conjugated AgNPs possessed a strong cytotoxic potency against human liver cancer. The in silico molecular docking results of prodigiosin and prodigiosin-conjugated AgNPs are congruent with the in vitro studies and these AgNPs can be considered as good inhibitors of mitogen-activated protein kinase 1 (MEK kinases). The study opened the possibility of using prodigiosin-conjugated AgNPs to increase the efficiency of liver cancer treatment.

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“…The concentration of prodigiosin required to inhibit biofilm formation of P. aeruginosa was within the range of 100–500 μM, which is at least five times higher than the reported concentrations required to induce apoptosis in human cancer cell lines (da Silva Melo et al, 2000 ; Montaner et al, 2000 ; Montaner and Perez-Tomas, 2001 ) and larger than the concentration of Streptorubin B to inhibit biofilm formation (Suzuki et al, 2015 ). Prodigiosin induces apoptotic effect in cancer cell lines through regulation of apoptopic and anti-apoptotic genes (Hassankhani et al, 2014 ), while the prodigiosin mediated biofilm inhibition has shown to rely on the production of reactive oxygen species.…”

Section: Discussionmentioning

confidence: 83%

Serratia Secondary Metabolite Prodigiosin Inhibits Pseudomonas aeruginosa Biofilm Development by Producing Reactive Oxygen Species that Damage Biological Molecules

et al. 2016

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Prodigiosin is a heterocyclic bacterial secondary metabolite belonging to the class of tripyrrole compounds, synthesized by various types of bacteria including Serratia species. Prodigiosin has been the subject of intense research over the last decade for its ability to induce apoptosis in several cancer cell lines. Reports suggest that prodigiosin promotes oxidative damage to double-stranded DNA (dsDNA) in the presence of copper ions and consequently leads to inhibition of cell-cycle progression and cell death. However, prodigiosin has not been previously implicated in biofilm inhibition. In this study, the link between prodigiosin and biofilm inhibition through the production of redox active metabolites is presented. Our study showed that prodigiosin (500 μM) (extracted from Serratia marcescens culture) and a prodigiosin/copper(II) (100 μM each) complex have strong RNA and dsDNA cleaving properties while they have no pronounced effect on protein. Results support a role for oxidative damage to biomolecules by H2O2 and hydroxyl radical generation. Further, it was demonstrated that reactive oxygen species scavengers significantly reduced the DNA and RNA cleaving property of prodigiosin. P. aeruginosa cell surface hydrophobicity and biofilm integrity were significantly altered due to the cleavage of nucleic acids by prodigiosin or the prodigiosin/copper(II) complex. In addition, prodigiosin also facilitated the bactericidal activity. The ability of prodigiosinto cause nucleic acid degradation offers novel opportunities to interfere with extracellular DNA dependent bacterial biofilms.

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Cytotoxicity of prodigiosin and benznidazole on V79 cells

Cited by 29 publications

References 33 publications

Mitochondrial dysfunction in Trypanosoma cruzi: the role of Serratia marcescens prodigiosin in the alternative treatment of Chagas disease

Mitochondrial dysfunction in Trypanosoma cruzi: the role of Serratia marcescens prodigiosin in the alternative treatment of Chagas disease

In silico and In vitro cytotoxic effect of prodigiosin-conjugated silver nanoparticles on liver cancer cells (HepG2)

Serratia Secondary Metabolite Prodigiosin Inhibits Pseudomonas aeruginosa Biofilm Development by Producing Reactive Oxygen Species that Damage Biological Molecules

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