Cytotoxicity of pharmaceuticals found in aquatic systems: Comparison of PLHC-1 and RTG-2 fish cell lines

Caminada, Daniel; Escher, Claudia; Fent, Karl

doi:10.1016/j.aquatox.2006.05.010

Cited by 104 publications

(43 citation statements)

References 39 publications

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“…Study results correlated well with the toxicity of the pharmaceuticals for Daphnia spp. Henschel et al ( 1997 ) and Laville et al ( 2004 ) found similar results to those of Caminada et al ( 2006 ) for fi sh cell lines PRTH (rainbow trout hepatocytes), PLHC-1, and BF-2 (bluegill fry) cells, in response to pharmaceutical exposure. Schnell et al ( 2009 ) examined the toxicity of 11 pharmaceuticals in the fi sh cell line RTL-W1 (rainbow trout liver), and observed that the antidepressants were the most toxic, and the anti-infl ammatory agents were the least toxic.…”

Section: In Vitro Assays and Computational Toxicologysupporting

confidence: 87%

“…In vitro cytotoxic assays using fi sh cell lines have been used to identify the potential acute and chronic effects of numerous xenobiotics (e.g., Babich and Borenfreund 1987 ;Saito et al 1991 ;Bruschweiler et al 1995 ) . Caminada et al ( 2006 ) provided an inclusive study in which the pharmaceutical effects of 34 different drugs were evaluated on fi sh cell lines PLHC-1 (hepatocellular carcinoma cell line) and RTG-2 (rainbow trout fi broblast-like cells). Study results correlated well with the toxicity of the pharmaceuticals for Daphnia spp.…”

Section: In Vitro Assays and Computational Toxicologymentioning

confidence: 99%

See 1 more Smart Citation

Human Pharmaceuticals in the Aquatic Environment: A Review of Recent Toxicological Studies and Considerations for Toxicity Testing

Brausch

Connors

Brooks

et al. 2012

Reviews of Environmental Contamination and Toxicology

133

119

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Section: In Vitro Assays and Computational Toxicologysupporting

confidence: 87%

Section: In Vitro Assays and Computational Toxicologymentioning

confidence: 99%

Human Pharmaceuticals in the Aquatic Environment: A Review of Recent Toxicological Studies and Considerations for Toxicity Testing

Brausch

Connors

Brooks

et al. 2012

Reviews of Environmental Contamination and Toxicology

133

119

View full text Add to dashboard Cite

“…Besides some popular in vitro models such as liver microsomes and primary hepatocytes (Winzer et al 2002;Henczová et al 2006), several established fish cell lines, including PLHC-1 (Celander et al 1996), RTG-2 (Araújo et al 2000), BB (Araújo et al 2000), RTL-W1 (Babín et al 2005), CIK (Tan et al 2008), and GCL (Li et al 2008;Lin et al 2006Lin et al , 2007 have successfully been used for acute toxicity assessment of a variety of environmental chemicals such as organotins, substituted phenols, and pharmaceuticals (Janošek et al 2006). The in vitro results showed a trend similar to the in vivo acute toxicity in fish (Fent 2001;Caminada et al 2006). These cells are therefore a promising tool in the toxicity screening and in the enzyme kinetics evaluation of pharmaceuticals.…”

Section: Introductionsupporting

confidence: 65%

Effects of fish CYP inducers on difloxacin N-demethylation in kidney cell of Chinese idle (Ctenopharyngodon idellus)

Yang

Wang

et al. 2010

Fish Physiol Biochem

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A drug-drug interaction occurs when the effect of one drug is altered by the presence of another drug which is generally associated with the induction of cytochrome P450s (CYPs) activity. Thus, unexpected treatment failures often happen resulting from inappropriate coadministration in fisheries. However, little information is available about CYP induction in fish. The reaction of difloxacin (DIF) biotransformation to sarafloxacin (SAR) belongs to N-demethylation catalyzed mainly by CYP(s). In order to supply useful information on CYP induction, the present study assessed the effects of fish-specific CYP inducers on DIF N-demethylation and enzyme kinetics in kidney cell of Chinese idle (CIK; grass carp (Ctenopharyngodon idellus)) by RP-HPLC. Results demonstrated that the amounts of SAR formation and enzymatic parameters Clint and Vmax were significantly increased due to beta-naphthoflavone (BNF) pretreatment. Therefore, we suggest that CYP1A may be involved in DIF N-demethylation in CIK. This study provides instructive information to ensure treatment success via avoiding CYP induction in fisheries.

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“…For example, cytotoxic results were obtained using the tetrazolium salt reduction (MTT) assay to determine the inhibition of mitochondrial succinate dehydrogenase and the inhibition of neutral red uptake into lysosomes based on cell membrane damage in fish cell lines (Fent and Hunn, 1996;Caminada et al, 2006). Laville et al (2004) found similar results determining the cytotoxicity of fluoxetine in PLHC-1 cells (5 μM FLU) and primary cultures of rainbow trout hepatocytes (66 μM FLU).…”

Section: Discussionmentioning

confidence: 52%

Cytotoxicity and mutagenicity of fluoxetine hydrochloride (Prozac), with or without vitamins A and C, in plant and animal model systems

Düsman¹,

Almeida²,

Mariucci³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Fluoxetine, commonly known as Prozac, is the first representative of the so-called new generation of antidepressants that promise efficacy, with few side effects, against deep depression, nervous bulimia, and anxiety. As there is a growing number of people suffering from anxiety and depression; consequently, the use of fluoxetine is also increasing. Verifying absence of drug effects such as cytotoxicity or mutagenicity is of great importance. Certain vitamins, such as vitamin A (retinol, retinoids) and vitamin C (ascorbic acid) protect and are extremely active against mutagens. We evaluated the cytotoxic and mutagenic activity of fluoxetine, with and without concomitant administration of vitamin A or C, in Allium cepa meristem cells and Wistar rat bone marrow cells. The A. cepa meristem cells Cytotoxicity and mutagenicity of fluoxetine showed fluoxetine cytotoxicity; concomitant treatment with vitamin A or C proved non-protective. Treatment of Wistar rats with fluoxetine intraperitoneally or via gavage did not affect cell division or cause clastogenic effects. Vitamin A and C did not affect the cytotoxicity or mutagenicity of fluoxetine in the rat cells.

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Cytotoxicity of pharmaceuticals found in aquatic systems: Comparison of PLHC-1 and RTG-2 fish cell lines

Cited by 104 publications

References 39 publications

Human Pharmaceuticals in the Aquatic Environment: A Review of Recent Toxicological Studies and Considerations for Toxicity Testing

Human Pharmaceuticals in the Aquatic Environment: A Review of Recent Toxicological Studies and Considerations for Toxicity Testing

Effects of fish CYP inducers on difloxacin N-demethylation in kidney cell of Chinese idle (Ctenopharyngodon idellus)

Cytotoxicity and mutagenicity of fluoxetine hydrochloride (Prozac), with or without vitamins A and C, in plant and animal model systems

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