Cytotoxicity of orthodontic materials assessed by survival tests in Saccharomyces cerevisiae

Limberger, Karen Martins; Westphalen, Graziela Henriques; Menezes, Luciane Macedo de; Medina-Silva, Renata

doi:10.1016/j.dental.2011.01.001

Cited by 21 publications

(27 citation statements)

References 41 publications

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“…For other side, L929 mouse fibroblasts [8], have been shown to behave similarly to primary human gingival fibroblasts and, therefore, are a suitable in-vitro model to the toxicity test [9][10][11][12]. Taken in conjunction, these findings are aligned with the notion that the orthodontic elastics citotoxicity may influence their clinical performance [4,13] with regard to tissue biocompatibility.…”

Section: Introductionsupporting

confidence: 75%

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

et al. 2015

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The aim of this study is to evaluate cytotoxicity between polyurethane and non-latex orthodontic elastomeric ligatures. Seven elastomeric ligatures of black-coloured from different manufactures (3 non-latex, 3 polyurethane and 1 latex) were divided into 7 groups of 10 elastics each: Group UK (nonlatex, 3M Unitek), Group TP (nonlatex, TP Orthodontics), Group AO (nonlatex, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident) and Group TC (latexControl, TP Orthodontics). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with 1-way analysis followed by the Tukey post hoc test were employed (p<.05). The results showed no statistically significant differences between Groups UK, TP and AO in all experimental times (p>.05). There was a statistically differences (p<.05) between the viability cell of Groups UK, TP and AO with the Groups O, M, TD and TC at 1, 2, 3 and 7 days. There was no statistically differences (p>.05) between the groups O, M and TD at in all experimental times. It can be concluded that the nonlatex elastomeric ligatures of Unitek, TP Orthodontics, and American Orthodontics showed higher cell viability compared to others ligatures.

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Section: Introductionsupporting

confidence: 75%

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

et al. 2015

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“…Cell lines [7], such as L929 mouse fibroblasts [8], have been shown to behave similarly to primary human gingival fibroblasts and, therefore, are a suitable in-vitro model to test the toxicity [9][10][11][12] of products used intra-orally during orthodontic treatment [13][14][15]. In order of evaluate the biological behavior of these materials in cell culture, the Objective of the present in vitro study was to test the cytotoxicity of polyurethane and latex-free Orthodontic elastomeric chains of different manufactures.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics

et al. 2012

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Allergy caused by latex proteins has been well documented. Thus, the study of non-latex materials, is necessary. The aim of this study is to evaluate the cytotoxicity of silver-coloured orthodontic chain elastics, of polyurethane and latex-free. Nine chain elastics from different manufactures (3 latex-free and 6 polyurethane) were divided into 9 groups of 10 elastics each: Group UK (Latex-free, 3M Unitek), Group TP (Látex-free, TP Orthodontics), Group AO (Látex-free, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident), Group UD (Polyurethane, Uniden), Group AZ (Polyurethane, Abzil) and Group AK (Polyurethane, Aditek). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with multiple comparisons and Tukey's test were employed (p<.05). The results showed no statistically significant differences between Groups UK, TP and AO in all experimental times (p>.05), except between the Groups UK and TP at 28 days (p<.05). There was significant statistically difference (p<.05) between the viability of the cells in the Groups O and TD with the Groups M, AZ, AK at 1 and 2 days. The polyurethane elastics showed lower cell viability in the first 48 hours, with increase on 3rd and 7th day, and viability similar to latex-free elastics at 28 days. It can be concluded that the latex-free chain elastic showed higher cell viability. The OrthoSource and Tecnident trademark showed lower cell viability the initial 48 hours.

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“…The bands are generally made of stainless steel and are composed of nickel, iron and chromium, and it is considered a biocompatible alloy [4, 5]. However, in several clinical situations, it is necessary to connect orthodontic wires to the bands, especially when auxiliary appliances, such as lingual arches and maxillary expanders, are made.…”

Section: Introductionmentioning

confidence: 99%

“…Biochemical and genetic similarities [26, 27] justify the use of yeast models to address a scientific question of clinical interest [18, 28–33]. However, few dental studies have used this microorganism for this purpose [34, 35], and only one was dedicated to efficiently evaluate orthodontic materials [4]. …”

Section: Introductionmentioning

confidence: 99%

Differences of Cytotoxicity of Orthodontic Bands Assessed by Survival Tests inSaccharomyces cerevisiae

Gonçalves

Menezes

Ribeiro

et al. 2014

BioMed Research International

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The aim of this study was to evaluate the cytotoxicity induced by orthodontic bands through survival tests on Saccharomyces cerevisiae, a microorganism that presents several genetic and biochemical characteristics similar to human cells. Three groups of bands were evaluated: silver soldered (SSB), laser soldered (LSB), and bands without any solder (WSB). Yeast cells were directly exposed to the bands and indirectly, when a previous elution of the metals in artificial saliva was performed. The negative control was composed of yeast cells or artificial saliva not exposed to any kind of metal. In the direct exposure experiments, all tested groups of bands induced a slight reduction in yeast viability compared to the control. This effect was more intense for the SSB, although not statistically significant. For the indirect exposure experiments, the SSB induced a statistically significant decrease in cell viability compared to the LSB. There were no significant differences between the survival rates of the negative control and the LSB group in both direct and saliva tests. SSBs were cytotoxic, whilst LSBs were not, confirming that laser soldering may be a more biocompatible alternative for use in connecting wires to orthodontic appliances.

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Cytotoxicity of orthodontic materials assessed by survival tests in Saccharomyces cerevisiae

Cited by 21 publications

References 41 publications

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics

Differences of Cytotoxicity of Orthodontic Bands Assessed by Survival Tests inSaccharomyces cerevisiae

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