Cytotoxicity of Nigella Sativa Seed Oil and Extract Against Human Lung Cancer Cell Line

Al-Sheddi, Ebtesam S.; Farshori, Nida Nayyar; Al-Oqail, Mai M.; Musarrat, Javed; Al-Khedhairy, Abdulaziz A.; Siddiqui, Maqsood A.

doi:10.7314/apjcp.2014.15.2.983

Cited by 61 publications

(48 citation statements)

References 26 publications

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“…There has been no published report on the effect of Abeliatriflora extract on the human lung adenocarcinoma cell line so far, but the growth inhibitory effects of other extracts on cancerous cells have been observed by different investigators on other human cancer cell lines (Li et al, 1995;Kim et al, 2002;Kumi-Diaka and Butler, 2000). Our results are in well agreements with the previous studies where the exposures of various natural products have been shown to induced significant cytotoxic effects against different cancer cell lines (Srividya et al, 2012;Al-Oqail et al, 2013Al-Sheddi et al, 2014;Farshori et al, 2014). Our results also correlate with previous findings showing the in vitro cytotoxicity in this concentration range (Abdullah et al, 2014).…”

Section: Discussionsupporting

confidence: 92%

“…Hence, the use of natural products has now been looked upon thoughtfully in the control of cancer. Plant derived natural products have received considerable attention in recent years due to the presence of various bioactive compounds and their diverse pharmacological properties including cytotoxic and cancer preventive effects (Ong et al, 1986;Owen et al, 2004;Omar, 2010;Patel et al, 2010;Karmakar et al, 2010;Al-Oqail et al, 2013;Farshori et al, 2013;Al-Sheddi et al, 2014;. The genus Abelia (Caprifoliaceae) consists of about eighty species distributed mostly in Himalaya and East Asia.A.triflorahas a wide distribution throughout from China to the Himalayas.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells

Al‐Taweel

Perveen²,

Fawzy³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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The present investigation was designed to assess the anticancer activity of six different leaf extracts (ethyl acetate, methanol, chloroform, petroleum ether, n-butanol, and water soluble) of Abelia triflora on A-549 human lung adenocarcinoma epithelial cells. A-549 cells were exposed to 10-1000 μg/ml concentrations of the leaf extracts of A. triflorafor 24 h and then percentage cell viability was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assay. The results showed that leaf extracts of A. triflora significantly reduced the viability of A-549 cells in a concentration-dependent manner. Decrease was recorded as 31% with ethyl acetate, 36% with methanol, 46% with chloroform, 54% with petroleum ether, 62% with n-butanol, and 63% with water soluble extracts at 1000 μg/ml each. Among the various plant extracts, ethyl acetate extract showed the highest decrease in the percentage cell viability, followed by methanol, chloroform, petroleum ether, n-butanol, and water soluble extracts. Our results demonstrated preliminary screening of anticancer activity of different soluble extracts of A. triflora extracts against A-549 cells, which can be further used for the development of a potential therapeutic anticancer agents.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells

Al‐Taweel

Perveen²,

Fawzy³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…Several studies demonstrate that traditional medicine could be a promising source in the development of a potential therapeutic anticancer drug (Pan and Ho, 2008;Al-Oqail et al, 2013;Farshori et al, 2013, Al-Sheddi et al, 2014. Herbal medicines have long been viewed as a source of curative remedy based on religious and cultural traditions (Ghazanfer, 1994;Pal and Shukla, 2003;Saetung et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…, Maqsood Ahmed Siddiqui 2,3 * 2011; Thoppil et al, 2013;Al-Oqail et al, 2013;Farshori et al, 2013, Al-Sheddi et al, 2014. Portulaca oleracea (purslane), is an annual green herbaceous medicinal plant widespread in temperate and tropical regions of the world (Yang et al, 2009).…”

Section: 3mentioning

confidence: 99%

Cytotoxicity Assessments of Portulaca oleracea and Petroselinum sativum Seed Extracts on Human Hepatocellular Carcinoma Cells (HepG2)

Farshori

Al-Sheddi²,

Al-Oqail³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…33,34 Recently, Al-Sheddi et al demonstrated that seed extract and seed oil of black cumin significantly reduced the cell viability of a human lung cancer cell line. 18 In the present study the cytotoxicity of BCSO was assessed by MTT assay, after exposure of the cells to 1.0-100 µ g/mL and 0.025-1.0 mg/mL BCSO for 24 h. Cytotoxicity data obtained from the MTT assays were extrapolated using the exponential equation…”

Section: Cytotoxicitymentioning

confidence: 99%

A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil

Şeleci¹,

Gümüş²,

Yavuz³

et al. 2015

Turk J Chem

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Abstract:The objectives of this study were to investigate the potential biological activities of black cumin seed oil (BCSO) and wheat germ oil (WGO) on different cell lines. Initially, commercially available BCSO and WGO obtained by supercritical carbon dioxide extraction were analyzed in terms of tocopherol, aliphatic alcohols, and thymoquinone content via HPLC and GC analysis. Cell free antioxidant activities and total phenolic content of both oils were detected by DPPH assay and Folin-Ciocalteu method, respectively. As well as the DPPH assay, the protective effect against reactive oxygen species (ROS) was determined by microscopic observation of ROS generation in NIH-3T3 cells with or without oil samples by using an oxidation-sensitive fluorescent dye, H2DCFDA. Cytotoxicity was assessed using an MTT assay. In the case of BCSO, after exposing cells to 0.025-1.0 mg/mL and 1.0-100 µ g/mL concentrations for 24 h, the IC 50 values of BCSO were 0.58, 0.51, 0.47, and 0.36 mg/mL for NIH-3T3, A549, U87, and HeLa cells, respectively. On the other hand, concentrations of WGO lower than 0.1 mg/mL did not cause a decrease in cell viability for all cell lines.Apoptotic and necrotic rates of these cell lines were investigated via flow cytometry. BCSO also exhibited proliferative efficacy for NIH-3T3 cells.

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Cytotoxicity of Nigella Sativa Seed Oil and Extract Against Human Lung Cancer Cell Line

Cited by 61 publications

References 26 publications

Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells

Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells

Cytotoxicity Assessments of Portulaca oleracea and Petroselinum sativum Seed Extracts on Human Hepatocellular Carcinoma Cells (HepG2)

A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil

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