Cytotoxicity of New Stilbenoids from Pholidota chinensis and Their Spin‐Labeled Derivatives

Abstract

Chemical investigation of the leave and stem of Pholidota chinensis Lindl has resulted in the isolation and characterization of three new stilbenoids. Their structures were established on the basis of spectroscopic evidence. A series of spin-labeled stilbene derivatives were synthesized. All of the new compounds were tested for cytotoxicity, and the results revealed that most of the spin-labeled stilbene derivatives showed superior cytotoxicity in vitro.

Isopentenylated Bibenzyls and Phenolic Compounds from Dendrobium chrysotoxum Lindl

“…were the same as those in wild plant. Among them, the chemical structures of above reported compounds were clearly determined by comparing their spectroscopic data with reported literature and identified as gigantol ( 3 ), [13] 3‐ O ‐methylgigantol ( 4 ), [14] moscatilin ( 5 ), [15] 4‐[2‐(3‐hydroxy‐4‐methoxyphenyl)ethyl]‐2,6‐dimethoxyphenol ( 6 ), [15] crepidatin ( 7 ), [15] chrysotoxine ( 8 ), [15] erianin ( 9 ), [16] isoamoenylin ( 10 ), [17] batatasin III ( 11 ), [13] tristin ( 12 ), [13] nobilin C ( 13 ), [18] moscatin ( 14 ), [19] 2,5‐dihydroxy‐4,9‐dimethoxyphenanthrene ( 15 ), [18] confusarin ( 16 ), [20] nudol ( 17 ), [21] fimbriatone ( 18 ), [22] 1,5,6,7‐tetramethoxy‐2‐hydroxyphenanthrenol ( 19 ), [23] 7‐hydroxy‐2,3,4‐trimethoxyphenanthrene ( 20 ), [24] 1,2,6,7‐tetrahydroxy‐4‐methoxyphenanthrene ( 21 ), [24] 2,4‐dihydroxy‐7‐methoxy‐9,10‐dihydrophenanthrene ( 22 ), [25] erianthridin ( 23 ), [26] 2,5‐dihydroxy‐4‐methoxy‐9,10‐dihydrophenanthrene ( 24 ), [27] 1,4,7‐trihydroxy‐5‐methoxy‐9 H ‐fluoren‐9‐one ( 25 ), [28] nobilone ( 26 ), [29] 6‐methylesculetin ( 27 ), [30] and homoeriodictiol ( 28 ), [31] respectively.…”

Separation and purification of 9,10-dihydrophenanthrenes and bibenzyls fromPholidota chinensisby high-speed countercurrent chromatography

“…Among them, 9,10‐dihydrophenanthrenes were reported as the major components of this genus . Many articles reporting the preparative separation and purification of 9,10‐dihydrophenanthrenes and bibenzyls by classical methods including silica gel, MCI, Sephadex LH‐20, preparative TLC, and preparative C 18 HPLC are tedious, time‐consuming, and require multiple chromatographic steps resulting in lower recovery and higher cost . Therefore, rapid separation and purification of bioactive principles from this genus received ever‐increasing attention.…”

Bioactive Diterpenes from Clerodendrum kaichianum

“…Compounds 2 and 3 were obtained after purifying Fr.3C on a Sephadex LH-20 column eluted with MeOH. Epoxy-11,14,17-trihydroxy-17(15→16)abeo-8,11,13-abietatrien-7- Cytotoxicity assay: The percentage of growth inhibition was determined using a MTT assay to measure viable cells, with minor modification [13][14][15][16][17]. A total of 5000-10,000 exponential phase cells per well was seeded onto a 96-well plate for 24 h, treated with compounds 1-6 at different concentrations for 72 h using cisplatin as a positive control.…”