Cytotoxicity of new resin‐, calcium hydroxide‐ and silicone‐based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines

Eldeniz, Ayçe Ünverdi; Mustafa, Kamal; Ørstavik, Dag; Dahl, Jon E.

doi:10.1111/j.1365-2591.2007.01211.x

Cited by 155 publications

(177 citation statements)

References 53 publications

(108 reference statements)

Supporting

Mentioning

124

Contrasting

Unclassified

Order By: Relevance

“…As reported by some authors (15), changes in culture and subculture media have varied intervals according to the used cell line. Therefore, the line should be properly considered when selecting a subculturing protocol, and choose the protocol that causes the least damage to the cells, as advocated by other authors.…”

Section: Discussionmentioning

confidence: 99%

“…For surface disinfection, test specimens were exposed to ultraviolet light (14,25), for approximately 30 h. However, some authors (14) used a 2-h period whereas others (25) used a 24-h period. This type of incubation used in the present study to assess the effect of the sealers on ECV-304 cells has been used elsewhere (13,15,(23)(24)(25). A previous study (13) also used ECV-304 cells that were incubated at 37ºC in 5% CO 2 and 100% humidity for 7 days and cultured in an F12 medium supplemented with 10% fetal bovine serum.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, it is a low-cost procedure that yields results within 48 h (12,13,18,19), and is therefore the most commonly used procedure for determining the cytotoxicity of different types of materials (20). Due to these characteristics and the endorsement by some authors (13,15,(21)(22)(23)(24)(25), this assay was used in this study for assessing the cytotoxicity of all the six endodontic sealers.…”

Section: Discussionmentioning

confidence: 99%

“…The MTT assay was used to assess cell viability (12,13,15,18,20,(22)(23)(24)(25). The MTT reduction tests were performed in quadruplicate at 12, 24, and 72 h using 96-well cell culture microplates.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

View full text Add to dashboard Cite

This study evaluated, in vitro, the cytotoxicity of six root canal sealers after 12, 24 and 72 h of contact time, using an endothelial ECV-304 cell line. The MTT assay was used for analysis of cell viability. Twelve specimens of each sealer were prepared and randomly assigned to 6 groups according to the commercial brands (n=4/time). A control group was also formed, which was not subjected to the contact with sealers. To assess the effects of sealers on endothelial cells, the specimens were placed in culture plate wells and incubated at 37°C with 5% CO 2 and 100% humidity. MTT assays were performed in quadruplicate after 12, 24 and 72 h of contact of the sealer specimens with monolayers. Statistical analysis was performed by two-way ANOVA with Bonferroni post-hoc test at a significance level of 5%. Analysis of absorbance in the experimental groups showed that GuttaFlow presented the lowest cytotoxicity, with a mean absorbance of 0.048, followed by Pulp Canal Sealer (0.038), Sealer 26 (0.038), Endo Densell (0.036) and Pulp Fill (0.035). The control group had a mean absorbance of 0.098. Based on the results, Endofill and GuttaFlow were the most and the least cytotoxic sealers, respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

View full text Add to dashboard Cite

show abstract

“…111 However, it has also been demonstrated that although Epiphany was cytotoxic it did not effect the viability of human leucocytes. 112 The reported cytotoxicity may be due to the leakage of uncured monomer from the oxygen inhibition layer, 100,101,113,114 or due to the degradation of the sealer in an aqueous environment. [115][116][117] The Bis-GMA and UDMA contained within the Epiphany bonding agent are another potential source of cytotoxicity.…”

Section: Biocompatibilitymentioning

confidence: 99%

Root canal filling using Resilon: a review

Shanahan

Duncan

2011

Br Dent J

View full text Add to dashboard Cite

Innovative silicate‐based cements for endodontics: A study of osteoblast‐like cell response

Gandolfi

Pagani

Perut

et al. 2008

J Biomedical Materials Res

View full text Add to dashboard Cite

Silicate-based filling materials were designed to obtain new endodontic sealers and root-end filling materials with adequate workability and consistency. Four different formulations (TC, TC 1%, TCf 1%, and TCf) were prepared incorporating calcium chloride as accelerant agent. A plasticizing compound (phyllosilicate) was added to TC 1% and TCf 1%. TC and TC 1% were prepared with water, whereas TCf and TCf 1% were mixed with a latex polymer as fluidizing agent. The aim of this study was to assess the in vitro biological compatibility of designed materials. White-MTA and AH Plus were tested as reference materials. Human osteoblast-like Saos-2 cells were challenged in short-term cultures (72 h) with solid materials and with material extracts in culture medium, and cell viability and number, cellular adhesion, and morphology were assessed. The new cements exerted no acute toxicity in the assay systems. Saos-2 like cells adhered and proliferated on solid samples of the experimental cements and MTA whilst AH Plus did not allowed cell growth. The extracts from the latex-containing cements showed some toxicity. By SEM analysis, osteoblast-like cells appeared adherent and spread on the new materials, and showed the maintenance of polygonal osteoblastic phenotype. Similar morphology was observed for cells on MTA, whereas only few cells were noted on the AH Plus surface. In conclusion, the new materials proved non toxic and supported the growth of bone-like cells, and resulted suitable to be used as endodontic sealers and root-end filling materials.

show abstract

Cytotoxicity of new resin‐, calcium hydroxide‐ and silicone‐based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines

Abstract: Toxicity varied but RC Sealer and GuttaFlow were the least toxic new sealers.

Cited by 155 publications

References 53 publications

Cytotoxicity of root canal sealers on endothelial cell cultures

Cytotoxicity of root canal sealers on endothelial cell cultures

Root canal filling using Resilon: a review

Innovative silicate‐based cements for endodontics: A study of osteoblast‐like cell response

Contact Info

Product

Resources

About