Cytotoxicity of naturally occurring phenolics and terpenoids from Kenyan flora towards human carcinoma cells

Kuete, Victor; Omosa, Leonidah Kerubo; Midiwo, Jacob O.; Karaosmanoğlu, Oğuzhan; Sivas, Hülya

doi:10.1016/j.jaim.2018.04.001

Cited by 23 publications

(7 citation statements)

References 27 publications

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“…The anti-cancer effect of flavonoids is due to the antioxidant effect, which, in turn, is dependent on a number of phenolic hydroxyl groups in their structure [64,65]. Similarly, many reports are available that show anti-cancer properties of terpenoids [66,67], as well as anti-inflammatory properties [68].…”

Section: Discussionmentioning

confidence: 99%

Tribulus terrestris Cytotoxicity against Breast Cancer MCF-7 and Lung Cancer A549 Cell Lines Is Mediated via Activation of Apoptosis, Caspase-3, DNA Degradation, and Suppressing Bcl-2 Activity

et al. 2022

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The primary objective of this research was to use flow cytometry to gain mechanistic insights into the cytotoxic effects of Tribulus terrestris extracts on breast cancer (MCF7) and lung cancer (A549) cell lines. T. terrestris was extracted using a Soxhlet apparatus in a progressive process. GC–MS was used to establish the phytochemical constituents. The amounts of phenolic compounds and flavonoids in the plant extracts were calculated using spectrophotometric analysis. The cytotoxicity of plant extracts was initially evaluated in non-malignant L929 cells, then in carcinogenic MCF-7 and A549 cell lines. Then, we performed an Annexin V assay, an anti-Bcl-2 assay, a Caspase-3 assay, and a DNA fragmentation (TUNEL) assay, using flow cytometry to investigate the underlying molecular processes. Based on the data, the methanolic extract of T. terrestris contained the highest amounts of phenolic compounds and flavonoids, with values of 169.87 µg GAE/g dwt and 160.12 µg QE/g dwt, respectively. Analysis by GC–MS revealed the presence of bioactive phytochemicals with proven cytotoxicity. Based on the MTT experiment, we determined that the IC50 values for the methanol extract’s effect on the viability of the MCF-7 and A549 cell lines were 218.19 and 179.62 µg/mL, respectively. The aqueous and methanol extracts were less cytotoxic when tested against the cancer-free L929 cell line (IC50 = 224.35 µg/mL). In both breast and lung cancer cells, the methanolic extract was found to activate caspase-3 and inhibit the Bcl-2 protein, resulting in early and late apoptosis and cell death via DNA damage. These findings point to cytotoxic effects of T. terrestris methanol extract against breast and lung cancer cell lines. Due to its potential as a source of anti-cancer chemotherapeutic medicines, T. terrestris warrants further investigation.

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Section: Discussionmentioning

confidence: 99%

Tribulus terrestris Cytotoxicity against Breast Cancer MCF-7 and Lung Cancer A549 Cell Lines Is Mediated via Activation of Apoptosis, Caspase-3, DNA Degradation, and Suppressing Bcl-2 Activity

et al. 2022

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“…The compounds in our study are derived from medicinal plants from 15 African countries including Algeria, Botswana, Burkina Faso, Cameroon, Congo, Ivory Coast, Egypt, Ghana, Kenya, Morocco, Mozambique, Nigeria, South Africa, Tunisia and Uganda. In this way, Cameroon, Morocco and [56]. Luteolin (3), isolated from Cyperus rotundus collected in Egypt, shows significant cytotoxicity against cancer cells HepG2 compared to the reference drug doxorubicin [57].…”

Section: Mechanisms Of Action Of Anti-cancer Molecules In African Med...mentioning

confidence: 99%

“…Fagaridine chloride (55), nitidine chloride (56) extracted from Fagara tessmannii (Cameroon) and Arborinine (64) isolated from Uapaca togoensis (Cameroon) inhibit the proliferation of CCRF-CEM cells by activating caspases 3/7, 8 and 9 and increasing ROS production. Arborinine (64) also blocks the cell cycle in G0/ G1 and S phases [101,102].…”

Section: Alkaloidsmentioning

confidence: 99%

In vitro activities and mechanisms of action of anti-cancer molecules from African medicinal plants: a systematic review

Adico

2024

Am J Cancer Res

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Cancer is one of the leading causes of death worldwide. In recent years, African countries have been faced with a rapid increase in morbidity and mortality due to this pathology. Management is often complicated by the high treatment costs, side effects and the increasing occurrence of resistance to treatments. The identification of new active ingredients extracted from endemic medicinal plants is definitively an interesting approach for the implementation of new therapeutic strategies: their extraction is often lower cost; their identification is based on an ethnobotanical history and a tradipratic approach; their use by low-income populations is simpler; this can help in the development of new synthetic molecules that are more active, more effective and with fewer side effects. The objective of this review is to document the molecules derived from African medicinal plants whose in vitro anti-cancer activities and the mechanisms of molecular actions have been identified. From the scientific databases Science Direct, PubMed and Google Scholar, we searched for publications on compounds isolated from African medicinal plants and having activity on cancer cells in culture. The data were analyzed in particular with regard to the cytotoxicity of the compounds and their mode of action. A total of 90 compounds of these African medicinal plants were selected. They come from nine chemical groups: alkaloids, flavonoids, polyphenols, quinones, saponins, steroids, terpenoids, xanthones and organic sulfides. These compounds have been associated with several cellular effects: i) Cytotoxicity, including caspase activation, alteration of mitochondrial membrane potential, and/or induction of reactive oxygen species (ROS); ii) Anti-angiogenesis; iii) Anti-metastatic properties. This review points out that the cited African plants are rich in active ingredients with anticancer properties. It also stresses that screening of these anti-tumor active ingredients should be continued at the continental scale. Altogether, this work provides a rational basis for the selection of phytochemical compounds for use in clinical trials.

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“…The phytochemical class terpenoids have been the subject of numerous studies (Kuete et al, 2019; Li et al, 2015; Nevzorova et al, 2017) revealing that specific terpenoids possess apoptotic potential against cancer cells and can regulate metastatic events. The inhibitory effects of terpenoids, including betulinic acid, impressic acid, isocunabic acid, and kaurenoic acid, on prostate cancer cells have been observed through (3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide analysis and western blotting.…”

Section: Introductionmentioning

confidence: 99%

Monoterpenoids: An upcoming class of therapeutic agents for modulating cancer metastasis

Mathur,

Meena,

Luqman

2023

Phytotherapy Research

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Monoterpenoids, a sub‐class of terpenoids, are secondary metabolites frequently extracted from the essential oils of aromatic plants. Their antitumor properties including antiproliferative, apoptotic, antiangiogenic, and antimetastatic effects along with other biological activities have been the subject of extensive study due to their diverse characteristics. In recent years, numerous investigations have been conducted to understand its potential anticancer impacts, specifically focusing on antiproliferative and apoptotic mechanisms. Metastasis, a malignancy hallmark, can exert either protective or destructive influences on tumor cells. Despite this, the potential antimetastatic and antiangiogenic attributes of monoterpenoids need further exploration. This review focuses on specific monoterpenoids, examining their effects on metastasis and relevant signaling pathways. The monoterpenoids exhibit a high level of complexity as natural products that regulate metastatic proteins through various signaling pathways, including phosphoinositide 3‐kinase/protein kinase B/mammalian target of rapamycin, mitogen‐activated protein kinase/extracellular signal‐regulated kinase/jun N‐terminal kinase, nuclear factor kappa B, vascular endothelial growth factor, and epithelial mesenchymal transition process. Additionally, this review delves into the biosynthesis and classification of monoterpenoids, their potential antitumor impacts on cell lines, the plant sources of monoterpenoids, and the current status of limited clinical trials investigating their efficacy against cancer. Moreover, monoterpenoids depict promising potential in preventing cancer metastasis, however, inadequate clinical trials limit their drug usage. State‐of‐the‐art techniques and technologies are being employed to overcome the challenges of utilizing monoterpenoids as an anticancer agent.

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Cytotoxicity of naturally occurring phenolics and terpenoids from Kenyan flora towards human carcinoma cells

Cited by 23 publications

References 27 publications

Tribulus terrestris Cytotoxicity against Breast Cancer MCF-7 and Lung Cancer A549 Cell Lines Is Mediated via Activation of Apoptosis, Caspase-3, DNA Degradation, and Suppressing Bcl-2 Activity

Tribulus terrestris Cytotoxicity against Breast Cancer MCF-7 and Lung Cancer A549 Cell Lines Is Mediated via Activation of Apoptosis, Caspase-3, DNA Degradation, and Suppressing Bcl-2 Activity

In vitro activities and mechanisms of action of anti-cancer molecules from African medicinal plants: a systematic review

Monoterpenoids: An upcoming class of therapeutic agents for modulating cancer metastasis

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