Cytotoxicity of mycotoxins evaluated by the MTT-cell culture assay

Hanelt, Monika; Gareis, M.; Kollarczik, Birgit

doi:10.1007/bf01138479

Cited by 168 publications

(121 citation statements)

References 29 publications

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…Cell viability was determined by the reduction of 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) (Chemicon, Temecula, Calif., U.S.A.) as described elsewhere (16). The experiment results are expressed as the mean of triplicatesϮstandard deviation (SD).…”

Section: Methodsmentioning

confidence: 99%

Piceatannol Prevents Lipopolysaccharide (LPS)‐Induced Nitric Oxide (NO) Production and Nuclear Factor (NF)‐κB Activation by Inhibiting IκB Kinase (IKK)

Islam

Hassan

et al. 2004

Microbiology and Immunology

View full text Add to dashboard Cite

., 48(10), [729][730][731][732][733][734][735][736] 2004 Abbreviations: ELISA, enzyme-linked immuno sorbent assay; Erk, extracellular signal regulated kinase; IKK, IκB kinase; iNOS, inducible isoform of NO synthase; LPS, lipopolysaccharide; MAP kinase, mitogen-activated protein kinase; MTT, 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide; NF-κB, nuclear factor-κB; NO, nitric oxide; SAPK/JNK, stressactivated protein kinase/c-Jun NH2-terminal kinase; SDS, sodium dodecylsulfate; TNF, tumor necrosis factor.

show abstract

Section: Methodsmentioning

confidence: 99%

Piceatannol Prevents Lipopolysaccharide (LPS)‐Induced Nitric Oxide (NO) Production and Nuclear Factor (NF)‐κB Activation by Inhibiting IκB Kinase (IKK)

Islam

Hassan

et al. 2004

Microbiology and Immunology

View full text Add to dashboard Cite

show abstract

“…Sp2 /0 cell line from Balb /C mice was used. Cell maintenance was as described in Hanelt et al 9 , except that Dubelcco's Modified Eagle's Medium (DMEM) was used instead of Modified Eagle's Medium (MEM). Aflatoxin B 1 , deoxynivalenol (DON) and zearalenone (ZEA) standards were from Sigma-Aldrich (catalogue numbers A6636, D0156 and Z2125, respectively).…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

“…The concentration of samples and mycotoxin standards ranged from 32 to 1000 kg/kg and 0.25 to 500 µg/g, respectively. Assays were performed according to Hanelt et al 9 The absorbance of cultures was measured at 490 nm with an ELISA reader (Multiscan ® ascent, Lab systems, Finland). Mean extinction values and standard deviations of each sample were compared with those of the corresponding controls and expressed as % cleavage activity in comparison to the cell controls (100%).…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

The Microbial Contamination, Toxicity and Quality of Turned and Unturned Outdoor Floor Malted Sorghum

Lefyedi

Marais

Dutton³

et al. 2005

Journal of the Institute of Brewing

View full text Add to dashboard Cite

Turned and unturned outdoor floor malted sorghum were studied for their total microbial contamination, nature and extent of contamination by moulds, cytotoxicity (IC 50 ) and quality in terms of diastatic power (DP). The presence of aflatoxins, fumonisins, deoxynivalenol and zearalenone were also investigated. Total microbial counts were high (10 7 -10 8 cfu /g) in both turned and unturned samples. All samples showed contamination by different moulds, with the dominant being Mucor species, Rhizopus oryzae, Fusarium moniliforme and Phoma sorghina as well as Aspergillus flavus and Alternaria alternata. The latter four are known for producing mycotoxins. Malt samples had very low cytotoxicity (IC 50 from 62.5 to >1000 kg/kg), though all contained fumonisins, deoxynivalenol and zearalenone at levels of < 0.25-2 µg/g, 15-20 and 10-15 µg/kg, respectively. Malt DP was generally lower in turned samples compared to unturned samples probably because the heat conserved in the latter ensured better germination conditions. Overall, turning during germination did not affect the microbial load, mould population and levels of deoxynivalenol and zearalenone in sorghum malt but decreased sorghum malt DP. Thus, alternative methods of controlling the sorghum malt microbial load should be sought.

show abstract

“…The MTT cell culture assay and a swine kidney target cell line were used as a direct method. Bulk samples were tested directly in the toxicity tests for presence of mycotoxins (19,20 by about half the adults (54%). The rate of preexisting and diagnosed allergy in patients was less than 20% and lower than in the comparison groups.…”

Section: Toxicity Screemningmentioning

confidence: 99%

Clinical experience and results of a Sentinel Health Investigation related to indoor fungal exposure.

Johanning

Landsbergis

Gareis

et al. 1999

Environ Health Perspect

View full text Add to dashboard Cite

This is a review of exposure conditions, clinical presentation, and morbidity of children and adults with indoor fungal exposure such as toxic Stachybotrys chartarum. Indoor exposure was characterized using different methods including microscopic, culture, cytotoxicity screening tests, and chemical analyses. Clinical case histories and physical and laboratory findings are presented of children (age < 18 years, n = 22; mean age 9 years; 60% females) and adults (age > 18 years, n = 125; mean age 39 years, 67% females) who consulted an environmental health specialty clinic. In the pediatric patients' exposure history, widespread fungal contamination of water-damaged building materials with known toxic or allergic fungi was identified. Primarily disorders of the respiratory system, skin, mucous membranes, and central nervous system were reported. Some enumeration and functional laboratory abnormalities, mainly of the lymphatic blood cells, were observed, although no statistically significant differences were found. IgE or lgG fungi-specific antibodies, used as exposure markers, were positive in less than 25% of all tested cases. In an evaluation of a symptomatic girl 11 years of age (sentinel case investigation) living in an apartment with verified toxigenic fungi (i.e., S. chartarum), several health indicators showed improvement after exposure cessation.

show abstract

Cytotoxicity of mycotoxins evaluated by the MTT-cell culture assay

Cited by 168 publications

References 29 publications

Piceatannol Prevents Lipopolysaccharide (LPS)‐Induced Nitric Oxide (NO) Production and Nuclear Factor (NF)‐κB Activation by Inhibiting IκB Kinase (IKK)

Piceatannol Prevents Lipopolysaccharide (LPS)‐Induced Nitric Oxide (NO) Production and Nuclear Factor (NF)‐κB Activation by Inhibiting IκB Kinase (IKK)

The Microbial Contamination, Toxicity and Quality of Turned and Unturned Outdoor Floor Malted Sorghum

Clinical experience and results of a Sentinel Health Investigation related to indoor fungal exposure.

Contact Info

Product

Resources

About