Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells

Deus, Gustavo De; Ximenes, Rafael Matos; Gurgel‐Filho, Eduardo Diogo; Plotkowski, Maria-Cristina; Coutinho-Filho, Tauby de Souza

doi:10.1111/j.1365-2591.2005.00987.x

Cited by 103 publications

(111 citation statements)

References 18 publications

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“…For surface disinfection, test specimens were exposed to ultraviolet light (14,25), for approximately 30 h. However, some authors (14) used a 2-h period whereas others (25) used a 24-h period. This type of incubation used in the present study to assess the effect of the sealers on ECV-304 cells has been used elsewhere (13,15,(23)(24)(25). A previous study (13) also used ECV-304 cells that were incubated at 37ºC in 5% CO 2 and 100% humidity for 7 days and cultured in an F12 medium supplemented with 10% fetal bovine serum.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, it is a low-cost procedure that yields results within 48 h (12,13,18,19), and is therefore the most commonly used procedure for determining the cytotoxicity of different types of materials (20). Due to these characteristics and the endorsement by some authors (13,15,(21)(22)(23)(24)(25), this assay was used in this study for assessing the cytotoxicity of all the six endodontic sealers.…”

Section: Discussionmentioning

confidence: 99%

“…These substances were also used in this study (10 mL solution containing 0.1% trypsin and 0.01% EDTA at 37ºC) for treating cells removed from cryogenic tubes and transferred to 25 cm 3 sterile bottles (13).…”

Section: Discussionmentioning

confidence: 99%

“…The MTT assay was used to assess cell viability (12,13,15,18,20,(22)(23)(24)(25). The MTT reduction tests were performed in quadruplicate at 12, 24, and 72 h using 96-well cell culture microplates.…”

Section: Discussionmentioning

confidence: 99%

“…These materials must fill the dentinal tubules, closely adhere to the organic and inorganic phases of the dentin, destroy or neutralize microorganisms and their byproducts, induce new cementum formation and strengthen the root canal system. From this perspective, all sealers in use can be considered inappropriate (13).…”

Section: Introdutionmentioning

confidence: 99%

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Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

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This study evaluated, in vitro, the cytotoxicity of six root canal sealers after 12, 24 and 72 h of contact time, using an endothelial ECV-304 cell line. The MTT assay was used for analysis of cell viability. Twelve specimens of each sealer were prepared and randomly assigned to 6 groups according to the commercial brands (n=4/time). A control group was also formed, which was not subjected to the contact with sealers. To assess the effects of sealers on endothelial cells, the specimens were placed in culture plate wells and incubated at 37°C with 5% CO 2 and 100% humidity. MTT assays were performed in quadruplicate after 12, 24 and 72 h of contact of the sealer specimens with monolayers. Statistical analysis was performed by two-way ANOVA with Bonferroni post-hoc test at a significance level of 5%. Analysis of absorbance in the experimental groups showed that GuttaFlow presented the lowest cytotoxicity, with a mean absorbance of 0.048, followed by Pulp Canal Sealer (0.038), Sealer 26 (0.038), Endo Densell (0.036) and Pulp Fill (0.035). The control group had a mean absorbance of 0.098. Based on the results, Endofill and GuttaFlow were the most and the least cytotoxic sealers, respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introdutionmentioning

confidence: 99%

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Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

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Bioceramics in Endodontics

Iliescu

Perlea

Tulus³

et al. 2019

Bioceramics and Biocomposites

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The Status of Mineral Trioxide Aggregate in Endodontics Education in Dental Schools in Turkey

Tanalp

Karapinar-Kazandag

Ersev

et al. 2012

Journal of Dental Education

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The aim of this study was to assess the current status of mineral trioxide aggregate (MTA) as an educational material in dental schools in Turkey. A survey was sent to senior members of the endodontic departments of seventeen dental schools; fourteen responded. All respondents reported that they used MTA in their clinical practice, with apexiication, perforations, retrograde illings, and root resorptions being the most frequently occurring treatment procedures. All reported that information was given to students regarding MTA mainly as part of the curriculum. The third and fourth years were the periods when MTA was introduced to students in most of the schools. Twelve schools reported that students had the opportunity to observe procedures in which MTA was used, but students had the chance to use the material in a very minor proportion of the schools, mainly under the supervision of clinical instructors. Ten schools agreed that MTA should be included in the regular endodontic curriculum. Financial constraints seemed to be the predominant reason for those who answered this question negatively, followed by dificult handling properties and low radiopacity of the material. Within the limitations of this study, it can be concluded that ways should be sought to prevent inancial dificulties from depriving dental students of the opportunity to receive information about contemporary methodologies such as MTA utilization.Dr. Tanalp is Associate Professor,

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Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells

Abstract: The two brands of MTA analysed, as well as the PC, initially showed a similar elevated cytotoxic effect that decreased gradually with time allowing the cell culture to become reestablished.

Cited by 103 publications

References 18 publications

Cytotoxicity of root canal sealers on endothelial cell cultures

Cytotoxicity of root canal sealers on endothelial cell cultures

Bioceramics in Endodontics

The Status of Mineral Trioxide Aggregate in Endodontics Education in Dental Schools in Turkey

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