Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines

Vijayarathna, Soundararajan; Sasidharan, Sreenivasan

doi:10.1016/s2221-1691(12)60237-8

Cited by 125 publications

(72 citation statements)

References 19 publications

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“…The biologically active components present in fenugreek seeds having the anti-inflammatory and antioxidant activities have also been studied (Liu et al, 2012). The morphological changes in the cells were observed more prominent in treated cells showing extensive blebbing and vacuolation suggesting autophagic mechanism of cell death (Vijayarathna, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…The evaluation and the discovery of new anticancer agents is long-term process that encompasses many steps. The step broaches with the screening for anticancer properties, followed by the isolation and identification of bioactive compounds obliged to anti cancer properties, toxicity estimation of the isolated compounds and finally in vivo anticancer activity testing to verify the aptitude of the compounds (Vijayarathna and Sreenivasan, 2012). Further plant derived natural products have received considerable attention in recent years due to their diverse pharmacological properties including cytotoxic and cancer preventive effects.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Cytotoxic Activity of Seed Oil of Fenugreek Against Various Cancer Cell Lines

Al-Oqail

Farshori²,

Al-Sheddi³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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In the present study, investigations were carried out to screen the anticancer activities of fenugreek seed oil against cancer cell lines (HEp-2, MCF-7, WISH cells), and a normal cell line (Vero cells). Cytotoxicity was assessed with MTT and NRU assays, and cellular morphological alterations were studied using phase contrast light microscopy. All cells were exposed toi 10-1000 μg/ml of fenugreek seed oil for 24 h. The results show that fenugreek seed oil significantly reduced the cell viability, and altered the cellular morphology in a dose dependent manner. Among the cell lines, HEp-2 cells showed the highest decrease in cell viability, followed by MCF-7, WISH, and Vero cells by MTT and NRU assays. Cell viability at 1000 μg/ml was recorded as 55% in HEp-2 cells, 67% in MCF-7 cells, 75% in WISH cells, and 86% in Vero cells. The present study provides preliminary screening data for fenugreek seed oil pointing to potent cytotoxicity against cancer cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Cytotoxic Activity of Seed Oil of Fenugreek Against Various Cancer Cell Lines

Al-Oqail

Farshori²,

Al-Sheddi³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…During the study conducted on the cytotoxic effect of E. natalensis on peripheral blood mononuclear cells, U937 monocytes and Chang liver cells, the plant extract showed low cellular toxicity on primary PBMC's (IC 50 131.3 ± 1.67 µg/ml), secondary U937 monocytes (IC 50 208.9 ± 10.3 µg/ml) and Chang liver cells (IC 50 148.2 ± 8.3 µg/ml). An IC 50 below 50 µg/ml has been considered to be moderately toxic and samples with a toxicity value higher than 100 µg/ml have been considered to be non-toxic (Vijayarathna and Sasidharan, 2012). In the study mentioned above, the cytotoxicity of the ethanolic extract of Solanum torvum was assessed on human fetal lung fibroblasts and the IC 50 of the extract was found to be 31.25 µg/ml (Nguta et al, 2016).…”

Section: In Vitro Antimycobacterial Antioxidant Cytotoxic Immunomomentioning

confidence: 99%

In vitro and In vivo antimycobacterial, hepatoprotective and immunomodulatory activity of Euclea natalensis and its mode of action

Lall

Kumar

Meyer

et al. 2016

Journal of Ethnopharmacology

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Ethnopharmacological relevance: The Natal gwarri or Natal ebony (Euclea natalensis A.DC.) is a deciduous tree found widespread throughout southern Africa, especially in Kwazulu-Natal and the southern cost. It has been widely used by indigenous communities such as the Zulus, Tsongas and Vendas for symptoms related to tuberculosis (TB). The decoctions made from the plant parts are administered for chest diseases to treat complications such as chest pains, bronchitis, pleurisy and asthma. TB is prevalent in immune-compromised patients and it is evident that TB-drugs cause hepatotoxicity. The objective of the present study was therefore to evaluate the antimycobacterial activity of the 2 ethanolic extract of E. natalensis against TB and its hepatoprotective and immunomodulatory activities. Materials and methods:The antimycobacterial, antioxidant, hepatoprotective, immunomodulatory activity and cytotoxicity of the ethanolic extract of the shoots of E. natalensis were determined in vitro. The mechanism of action of the antituberculosis activity was determined by investigating the inhibitory effect on mycothiol disulfide reductase enzyme. Furthermore, the acute, sub-acute toxicity (50-2000 mg/kg) and antimycobacterial effect (300 mg/kg) of E. natalensis shoot extract were investigated in Balb/c mice.Hepatoprotective activity of the extract (50-150 mg/kg) was evaluated on isoniazid and rifampicin (50 mg/kg; i.p.) induced hepatic damage in a rat model. Results:The minimum inhibitory concentration of the extract was found to be 125 µg/ml against Mycobacterium tuberculosis. The extracts fifty percent inhibitory concentration (IC 50 ) against 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical was found to be 22.55 µg/ml. The plant showed a hepatoprotective effect (50% at 12.5 µg/ml) and the ability to increase Thelper 1 cell cytokines; Interleukin 12, Interleukin 2 and Interferon α by up to 12 fold and the ability to decrease the T-helper 2 cell cytokine Interleukin 10 4 fold when compared to baseline cytokine production. No cellular toxicity was observed in primary peripheral blood mononuclear cells (PBMC's) and two secondary cell lines; U937 monocytes and Chang liver cells (a derivative of the HepG2 cell line). During mechanistic studies, the extract showed a 50% inhibition of mycothiol reductase activity at 38.62 µg/ml. During the acute and subacute studies, E. natalensis exhibited no toxic effect and the fifty percent lethal dose (LD 50 ) was established to be above 2000 mg/kg. The extract was able to reduce the mycobacterial load (1.5-fold reduction) in infected mice. Isoniazid and rifampicin caused significant hepatic damage in rats, and the extract was able to reduce the toxicity by 15% and 40% at 50 and 150 mg/kg respectively. Conclusion:The present study supports the traditional usage of the plant against tuberculosis symptoms. The study showed the ability of E. natalensis shoot extract to inhibit mycobacterial growth, stimulate an appropriate immune response and have a hepatic protective effect. Due to the ...

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“…Gul et al [27] while determining antioxidant and antiproliferative activities of Abrus precatorius leaf extract, observed that the extract selectively inhibited proliferation of HCC cell lines among others while sparing macrophage cells. In a corresponding work, methanolic extracts of Elaeis guineensis have shown selective antiproliferative effects against MCF-7 and vero cell lines as demonstrated by Vijayarathna and Sasidharan [28].…”

Section: Discussionmentioning

confidence: 86%

In Vitro Antiproliferative Activity of Aqueous Root Bark Extract of Cassia abbreviata (Holmes) Brenan

SM¹,

RC²

2016

J Cancer Sci Ther

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Cancer is referred to as uncontrolled abnormal proliferation of body cells. Currently cancer treatment and management is a challenge due to complexity of the disease, toxicity of chemotherapy, unaffordability of treatment and severe side effects. Therefore, it is imperative to investigate complementary and alternative medicine for leads and development of anticancer drugs. Cassia abbreviate (C. abbreviata) has been traditionally used for its ethnotherapeutic properties and pharmacological activities. It has been reported to possess antiproliferative activity, though there is no scientific evidence to validate this claim. The main objective of this study was to determine the antiproliferative activity of aqueous root bark extract of C. abbreviata on hepatocellular carcinoma (HCC), vero, and macrophage cell lines in vitro. The antiproliferative activity of aqueous root bark extract of C. abbreviata was determined using MTT assay. The results showed that aqueous root bark extract of C. abbreviata has antiproliferative activity against HCC, vero and macrophage cell lines. The extract had the highest antiproliferative activity against HCC cell line with an IC 50 of 1.49 µg/ml as compared to 81.08 µg/ml and 128.38 µg/ml in macrophages and vero cells respectively. The IC 50 observed on non-cancerous normal cells (macrophages and vero cells) indicated that the plant extract had little antiproliferative effects on normal cells hence regarded as safe. The extract contained flavonoids, phenols, tannins and saponins. In conclusion the antiproliferative activity of aqueous root bark extract of Cassia abbreviata observed could be attributed to the phytochemicals present in this plant extract. The results of this study, validates the claim that aqueous root bark extract of C. abbreviata has antiproliferative activity and justifies its use in herbal medicine.

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Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines

Cited by 125 publications

References 19 publications

In Vitro Cytotoxic Activity of Seed Oil of Fenugreek Against Various Cancer Cell Lines

In Vitro Cytotoxic Activity of Seed Oil of Fenugreek Against Various Cancer Cell Lines

In vitro and In vivo antimycobacterial, hepatoprotective and immunomodulatory activity of Euclea natalensis and its mode of action

In Vitro Antiproliferative Activity of Aqueous Root Bark Extract of Cassia abbreviata (Holmes) Brenan

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