Cytotoxicity of Luffa cylindrica (L.) M.Roem. extract against circulating cancer stem cells in hepatocellular carcinoma

Abdelsalam, Ibrahim M.; Awadein, Nihal El-Sayed; Ashour, Mahmoud

doi:10.1016/j.jep.2018.09.034

Cited by 26 publications

(15 citation statements)

References 22 publications

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“…Previous studies reported LC had abundant dietary fibers and beneficial nutrients that displayed antibacterial and hepatic protective roles (31,45). However, the antiobesity effects of LC, and whether these effects depended upon the gut microbiota, were unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Previous works demonstrated that the whole fruit of LC could be used as folk medicine for treating cough, fever, dyspnea, and subcutaneous swelling (30). Moreover, LC extracts played anticancer, antitoxicity, and anti-inflammation roles in vitro (31,32). Although these studies suggest LC contains more natural phytochemical compounds that effectively benefit human health, whether the gut microbiota determines the improvement roles of LC is less known and still needs to be discovered.…”

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confidence: 99%

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Gut microbiota determines the prevention effects of Luffa cylindrica (L.) Roem supplementation against obesity and associated metabolic disorders induced by high‐fat diet

Zhang

Shi

et al. 2019

FASEB j.

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The gut microbiota, identified as the target for vegetables, can affect the development of obesity and associated metabolic syndromes. As a medicinal and edible plant, Luffa cylindrica (L.) Roem (LC) has abundant nutrients that can effectively reduce obesity risk. However, the interaction between the prevention effects of LC against obesity and the modulating gut microbiota of LC remain obscure. This study demonstrated LC supplementation improved high‐fat diet (HFD)–induced gut microbiota dysbiosis and significantly enhanced short‐chain fatty acid (SCFA)–producing bacteria (e.g., Blautia) along with SCFA content accumulation in the gut. Meanwhile, LC supplementation substantially restored gut barrier damage in long‐term HFD treatment Moreover, LC supplementation improved HFD‐induced overweight, hyperlipidemia, insulin resistance, and chronic inflammation. Gene expression profiles showed that LC displayed an important impact on hepatic lipid transport and lipid synthesis (sterol regulatory element binding transcriptional factor 1c–peroxisome proliferator‐activated receptor γ signaling pathway). More importantly, an antibiotic treatment experiment demonstrated that the beneficial effects of LC in reducing obesity risk largely depended on the gut microbiota, especially SCFA‐producing bacteria (e.g., Blautia). Therefore, LC supplementation improved gut microbiota dysbiosis via enhancing SCFA‐producing bacteria (e.g., Blautia), maintained gut barrier integrity, and alleviated the development of obesity. Overall, LC would provide a potential dietary intervention strategy against obesity and enteral homeostasis dysbiosis through modulating the gut microbiota.—Zhang, L., Shi, M., Ji, J., Hu, X., Chen, F. Gut microbiota determines the prevention effects of Luffa cylindrica (L.) Roem supplementation against obesity and associated metabolic disorders induced by high‐fat diet. FASEB J. 33, 10339–10352 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Gut microbiota determines the prevention effects of Luffa cylindrica (L.) Roem supplementation against obesity and associated metabolic disorders induced by high‐fat diet

Zhang

Shi

et al. 2019

FASEB j.

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“…The few successful studies included intermediate/advanced cancer patients and reported similar culture conditions including normoxia, Matrigel TM solution, and DMEM (with or without 10% fetal bovine serum). cCSC isolation methods varied widely, including negative selection with magnetic beads [ 23 , 116 ] and positive selection by microfluidic chip [ 72 ] or fluorescent activated cell sorter [ 117 ]. A success rate of 86% to 100% has been reported with a median cell survival time of 7–14 days, although a publication bias is highly probable.…”

Section: Cell Culture Of Hcc Ctcsmentioning

confidence: 99%

Circulating Tumor Cells in Hepatocellular Carcinoma: A Comprehensive Review and Critical Appraisal

Espejo-Cruz

González-Rubio

Olaya

et al. 2021

IJMS

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Hepatocellular carcinoma (HCC) is the fifth most common neoplasm and a major cause of cancer-related death worldwide. There is no ideal biomarker allowing early diagnosis of HCC and tumor surveillance in patients receiving therapy. Liquid biopsy, and particularly circulating tumor cells (CTCs), have emerged as a useful tool for diagnosis and monitoring therapeutic responses in different tumors. In the present manuscript, we evaluate the current evidence supporting the quantitative and qualitative assessment of CTCs as potential biomarkers of HCC, as well as technical aspects related to isolation, identification, and classification of CTCs. Although the dynamic assessment of CTCs in patients with HCC may aid the decision-making process, there are still many uncertainties and technical caveats to be solved before this methodology has a true impact on clinical practice guidelines. More studies are needed to identify the optimal combination of surface markers, to increase the efficiency of ex-vivo expansion of CTCs, or even to target CTCs as a potential therapeutic strategy to prevent HCC recurrence after surgery or to hamper tumor progression and extrahepatic spreading.

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“…Alcalase or tryptic protein hydrolysates in its seeds are an effective treatment for diabetes and hypertension 6 . Additionally, the leaves, seeds, and fruits of sponge gourd have been used for the treatment of various diseases, including inflammatory diseases, diarrhea, and viral infections 7,8 , and the triterpenoids isolated from sponge gourd (sapogenins 1 and 2) exhibit immunomodulatory activity 9 . These findings explain the high medicinal value of sponge gourd, making it a focus of recent scientific research.…”

Section: Introductionmentioning

confidence: 99%

A high-quality sponge gourd (Luffa cylindrica) genome

Zhao

Gong

et al. 2020

Hortic Res

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Sponge gourd (Luffa cylindrica) is an important cultivated vegetable and medicinal plant in the family Cucurbitaceae. In this study, a draft genome sequence of the sponge gourd inbred line P93075 was analyzed. Using Illumina, PacBio, and 10× Genomics sequencing techniques as well as new assembly techniques such as FALCON and chromatin interaction mapping (Hi-C), a chromosome-scale genome of approximately 656.19 Mb, with an N50 scaffold length of 48.76 Mb, was generated. From this assembly, 25,508 protein-coding gene loci were identified, and 63.81% of the whole-genome consisted of transposable elements, which are major contributors to the expansion of the sponge gourd genome. According to a phylogenetic analysis of conserved genes, the sponge gourd lineage diverged from the bitter gourd lineage approximately 41.6 million years ago. Additionally, many genes that respond to biotic and abiotic stresses were found to be lineage specific or expanded in the sponge gourd genome, as demonstrated by the presence of 462 NBS-LRR genes, a much greater number than are found in the genomes of other cucurbit species; these results are consistent with the high stress resistance of sponge gourd. Collectively, our study provides insights into genome evolution and serves as a valuable reference for the genetic improvement of sponge gourd.

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Cytotoxicity of Luffa cylindrica (L.) M.Roem. extract against circulating cancer stem cells in hepatocellular carcinoma

Cited by 26 publications

References 22 publications

Gut microbiota determines the prevention effects of Luffa cylindrica (L.) Roem supplementation against obesity and associated metabolic disorders induced by high‐fat diet

Gut microbiota determines the prevention effects of Luffa cylindrica (L.) Roem supplementation against obesity and associated metabolic disorders induced by high‐fat diet

Circulating Tumor Cells in Hepatocellular Carcinoma: A Comprehensive Review and Critical Appraisal

A high-quality sponge gourd (Luffa cylindrica) genome

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