Cytotoxicity of gold nanoparticles with different structures and surface-anchored chiral polymers

Deng, Jun; Mengyun, Yao; Gao, Changyou

doi:10.1016/j.actbio.2017.01.082

Cited by 55 publications

(42 citation statements)

References 61 publications

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“…18,28,29 Unfortunately, aforementioned metal NPs are toxic for human cells, which restricts their application in the clinic. 30,31 As opposed to aforementioned metal and others such as aluminum and antimony, bismuth is considered to be nontoxic. 32 In medicine, bismuth subsalicylate is used to treat diarrhea without cytotoxic reports.…”

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confidence: 99%

In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells

Hernandez-Delgadillo

García-Cuéllar

Sánchez-Pérez

et al. 2018

IJN

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AimThe objective of this study was to evaluate the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) on breast cancer cells.Materials and methodsThe effect of varying concentrations of BisBAL NPs was evaluated on human MCF-7 breast cancer cells and on MCF-10A fibrocystic mammary epitheliocytes as noncancer control cells. Cell viability was evaluated with the MTT assay, plasma membrane integrity was analyzed with the calcein AM assay, genotoxicity with the comet assay, and apoptosis with the Annexin V/7-AAD assay.ResultsBisBAL NPs were spherical in shape (average diameter, 28 nm) and agglomerated into dense electronic clusters. BisBAL NP induced a dose-dependent growth inhibition. Most importantly, growth inhibition was higher for MCF-7 cells than for MCF-10A cells. At 1 µM BisBAL NP, MCF-7 growth inhibition was 51%, while it was 11% for MCF-10A; at 25 µM BisBAL NP, the growth inhibition was 81% for MCF-7 and 24% for MCF-10A. With respect to mechanisms of action, a 24-hour exposure of 10 and 100 µM BisBAL NP caused loss of cell membrane integrity and fragmentation of tumor cell DNA. BisBAL NPs at 10 µM were genotoxic to and caused apoptosis of breast cancer cells.ConclusionBisBAL NP-induced growth inhibition is dose dependent, and breast cancer cells are more vulnerable than noncancer breast cells. The mechanism of action of BisBAL NPs may include loss of plasma membrane integrity and a genotoxic effect on the genomic DNA of breast cancer cells.

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confidence: 99%

In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells

Hernandez-Delgadillo

García-Cuéllar

Sánchez-Pérez

et al. 2018

IJN

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“…Gold(III) chloride hydrate (HAuCl 4 ) and sodium citrate (C 6 H 5 Na 3 O 7 ·2H 2 O) were purchased from Sinopharm group Co. Ld. Poly(acryloyl- l -valine) ( l -PAV) ( M w 4926 Da, polydispersity 1.24), poly(acryloyl- d -valine) ( d -PAV) ( M w 4997 Da, polydispersity 1.15) were synthesized and were characterized according our previous works [ 17 , 23 ]. High glucose dulbecco’s modified eagle medium (DMEM) and fetal bovine serum (FBS) were obtained from HyClone (USA).…”

Section: Methodsmentioning

confidence: 99%

“…For instance, almost all of amino acids (except glycine) in proteins are “left-handed” ( l -), while all sugars in DNA and RNA are “right-handed” ( d -) [ 16 ]. Our and other previous studies have demonstrated chirality-dependent nature of cellular uptake [ 17 ], cell adhesion [ 18 ], cell differentiation [ 19 , 20 ], protein adsorption [ 21 , 22 ] (i.e., amount and affinity) and cytotoxicity [ 23 ] on flat substrates or nanoparticles coated with chiral molecules. More recently, two studies observed that NPs with surface-anchored chiral molecules could modulate the autophagy [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 92%

Chiral polymer modified nanoparticles selectively induce autophagy of cancer cells for tumor ablation

Yuan

Zhang

et al. 2018

J Nanobiotechnol

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BackgroundAutophagy regulation through exogenous materials has aroused intensive attention to develop treatment protocols according to diverse human diseases. However, to the best of our knowledge, few examples have been reported to selectively control autophagy process and ultimately achieve efficient therapeutic potential.ResultsIn this study, monolayers of poly (acryloyl-l, d and racemic valine) (l-PAV-AuNPs, d-PAV-AuNPs and l/d-PAV-AuNPs) chiral molecules were anchored on the surfaces of gold nanoparticles (PAV-AuNPs), and the subsequent chirality-selective effects on autophagy activation were thoroughly studied. The cytotoxicity induced by PAV-AuNPs towards MDA-MB-231 cells (Breast cancer cells) was achieved mainly through autophagy and showed chirality-dependent, with d-PAV-AuNPs exhibiting high autophagy-inducing activity in vitro and in vivo. In contrast, the PAV-AuNPs exhibited autophagy inactivation for normal cells, e.g., 3T3 fibroblasts and HBL-100 cells. The chirality-selective autophagy activation effect in MDA-MB-231 cells was likely attributed to the chirality-variant ROS generation, cellular uptake and their continuous autophagy stimulus. Furthermore, the intratumoral injection of d-PAV-AuNPs could largely suppress the tumor growth but exhibit negligible toxicity in vivo.ConclusionsAs the first exploration on stereospecific NPs for autophagy induction, this work not only substantiates that chiral polymer coated NPs can selective induce autophagy-specific in cancer cells and achieve a high tumor eradication efficiency in vivo, but also opens up a new direction in discovering unprecedented stereospecific nanoagents for autophagy-associated tumor treatment.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0383-9) contains supplementary material, which is available to authorized users.

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“…NPs were thoroughly characterized to determine their particle size, morphology, structure, composition and optical properties. The biological profile of both NPs was evaluated by examining interactions with bacteria, proteins and DNA while bioaffinity, biocompatibility and toxicity of NPs were also estimated [22,23,24]. Antibacterial activity was investigated against the Grampositive B. subtilis, B. cereus and S. aureus and the Gram-negative E. coli and X. campestris.…”

Section: Introductionmentioning

confidence: 99%

Biological relevance of CuFeO2 nanoparticles: Antibacterial and anti-inflammatory activity, genotoxicity, DNA and protein interactions

Antonoglou

Lafazanis

Mourdikoudis

et al. 2019

Materials Science and Engineering: C

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Heterometal oxide nanoparticles of bioessential metals are shedding new light to nanoparticleinspired bioapplications. Pairing bioreactive elements like copper and iron can affect the redox dynamic and biological profile of the nanomaterial. Given the complexity of physicochemical properties, biological activity and toxicity concerns, extensive exploration is demanded, especially when active and less active oxidation states participate as in case of cuprous-ferric delafossite CuFeO2 (copper(I)-iron(III)), a less widespread nanomaterial. In that vein, CuFeO2 nanoparticles were synthesized and biological profile was evaluated in comparison with cuprous oxide (Cu2O NPs) counterpart, an already established antimicrobial agent. Interactions with bacteria, proteins and DNA were examined. Cu2O NPs exhibited stronger antibacterial activity (IC50 <25 μg/ml) than CuFeO2 NPs (IC50>100 μg/ml). In vitro exposure of nanoparticles on plasmid DNA unveiled toxicity in the form of DNA damage for Cu2O and enhanced biocompatibility for CuFeO2 NPs. Genotoxicity estimated by the frequency of sister chromatid exchanges, cytostaticity based on the proliferating rate indices and cytotoxicity based on the mitotic indices at human peripheral lymphocyte cultures were all significantly lower in the case of CuFeO2 NPs. Furthermore, through in vitro albumin denaturation assay, CuFeO2 NPs showed better performance in protein denaturation protection, correlating in superior antiinflammatory activity than Cu2O and similar to acetylsalicylic acid. Synergy of copper(I)iron(III) in nanoscale is apparent and gives rise to fruitful bioapplications and perspectives.

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Cytotoxicity of gold nanoparticles with different structures and surface-anchored chiral polymers

Cited by 55 publications

References 61 publications

In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells

In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells

Chiral polymer modified nanoparticles selectively induce autophagy of cancer cells for tumor ablation

Biological relevance of CuFeO2 nanoparticles: Antibacterial and anti-inflammatory activity, genotoxicity, DNA and protein interactions

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