Cytotoxicity of fluoride

Holland, Roy I.

doi:10.3109/00016358009003481

Cited by 22 publications

(9 citation statements)

References 101 publications

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“…) calf serum, 20 mM HEPES, and 3 mM NaF. Holland 1979;Holland 1980) is the most fluoride sensitive process in liver cells in vivo, is not clear, but the cellular defence mechanism towards fluoride could be observed in vitro as decreased fluoride sensitivity of the protein synthesis. Cells cultured in vitro may, by prolonged exposure to increasing concentrations of fluoride, develop resistance to otherwise lethal fluoride concentrations (Carlson & Suttie 1967;Quissell & Suttie 1972;Hongslo et al 1974;Holland & Hongslo 1978a).…”

Section: Discussionmentioning

confidence: 99%

Reduced Fluoride Sensitivity of Liver Cells from Rats Chronically Exposed to Fluoride

Hongslo¹,

Hongslo

Hasvold

et al. 1980

Acta Pharmacologica et Toxicologica

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Absrruct:The fluoride sensitivity, determined as effect on protein synthesis (incorporation of "C-leucine), of liver and kidney cells in suspension culture was explored. The cells were freshly prepared by collagenase perfusion from rats given drinking water with or without addition of 100 p.p.m. (5.26 mM) fluoride for 9-28 weeks. The fluoride sensitivity of the liver cells from rats given fluoridated water for more than 9 weeks was decreased compared to cells from control rats, whereas the fluoride sensitivity of the kidney cells from fluoride exposed and control rats appeared similar. Fluoride resistance (i.e. decreased sensitivity)may thus develop also in cells in vivo. When exposed to 3 mM NaF for 1 hour the intracellular concentration of fluoride in liver cells from fluoride exposed and control animals was similar.

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Section: Discussionmentioning

confidence: 99%

Reduced Fluoride Sensitivity of Liver Cells from Rats Chronically Exposed to Fluoride

Hongslo¹,

Hongslo

Hasvold

et al. 1980

Acta Pharmacologica et Toxicologica

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“…Since Toxoplasma (both live and dead) are internalized rapidly by macrophages, the experiment was repeated with sodium fluoride to block phagocytosis of the organisms. Sodium fluoride has previously been shown to prevent entry of latex beads (40) and yeast cells (41) into macrophages, although it may have a wide range of effects of cells (42). Monolayers of rat alveolar macrophages were incubated for 1 h with medium that contained acridine orange (10 pg/ml) and 5 m M sodium fluoride, preliminary experiments having shown this to be the lowest concentration of fluoride that would prevent ingestion of Toxoplasma by these cells.…”

Section: Viability Of Toxoplasma Attached To the Surface Of Rat Alveomentioning

confidence: 99%

Oxygen-independent killing by alveolar macrophages.

Catterall¹,

Sharma²,

Remington³

1986

The Journal of Experimental Medicine

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Alveolar macrophages play a key role in defense of the host against pulmonary infection (1-6). Their clinical importance is emphasized by the high incidence of life-threatening pneumonia (7,8) in patients with abnormal macrophage function (6) or impaired ceU-mediated immunity (6-9). However, the mechanisms by which alveolar macrophages kill microorganisms are poorly understood.We recently examined the mechanism by which human alveolar macrophages kill the intracellular parasite, Toxoplasma gondii (10). We chose this organism because it causes pneumonia in immunosuppressed patients (11-14) but not in healthy individuals (11), and because macrophages play an important part in the host's resistance to this organism (5,(15)(16)(17). Our studies showed that killing of T. gondii by human alveolar macrophages occurred without involvement of toxic metabolites of oxygen (Catterall, J. R., and J. S. Remington, manuscript in preparation). This suggested that previous studies of intracellular killing by normal macrophages might have limitations as models for the human alveolar macrophage, since most of them (18-20, and reviewed in 21), including all those that employed T. gondii (18-23), have emphasized the overriding importance of oxidative killing mechanisms. Nonoxidative antimicrobial activity has been shown in subcellular macrophage fragments (21, 24) and in oxidatively deficient cells (19,(21)(22)(23)25), but in none of the previously described models using normal intact macrophages has the killing of intracellular parasites been reported as nonoxidative.To facilitate the study of nonoxidative killing by alveolar macrophages, we have sought a laboratory animal model relevant to the human alveolar macrophage. Since rats more closely resemble human subjects in their resistance to T. gondii than many other animals (26, 27), we have examined the interaction between T. gondii and rat alveolar macrophages in vitro. The studies reported here indicate that rat alveolar macrophages, like those from human subjects, also kill T. gondii, and by nonoxidative mechanisms. They suggest that nonoxidative antimicrobial mechanisms may be important in alveolar macrophages, and they provide a convenient model for the study of such mechanisms.

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“…In this context, the different amounts and wide range of concentrations of fluoride in currently used preventive agents were reviewed; studies were cited which reported that oral retention and ingestion of fluoride can occur from professional topical fluoride treatments, fluoride-containing dentifrices, and fluoride mouthrinses; and possible effects of inadvertent fluoride ingestion were described. These effects which are influenced by the age of the patient, the amount of fluoride consumed, and whether ingestion is acute or chronic -principally include digestive episodes (e.g., nausea and vomiting), biochemical and cytological tissue effects, and enamel fluorosis (Holland, 1980;M0ller, 1982).…”

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confidence: 99%

Topical Fluorides: A Discussion of Risks and Benefits

Ripa

1987

J Dent Res

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Inadvertent ingestion of fluoride from topical procedures poses the risk of dental fluorosis in age-susceptible users. This risk can be minimized for mouthrinsing by restricting it to those above the age-susceptible limit. Operators administering topical fluoride treatments can take practical precautions that have been shown to reduce the amount of fluoride retained and, thus, reduce ingestion. Clinical and epidemiological studies have failed to establish that dentifrice ingestion by young children constitutes a serious fluorosis risk. Discussion of risk must consider the effect of perceived risks upon the public.

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Cytotoxicity of fluoride

Cited by 22 publications

References 101 publications

Reduced Fluoride Sensitivity of Liver Cells from Rats Chronically Exposed to Fluoride

Reduced Fluoride Sensitivity of Liver Cells from Rats Chronically Exposed to Fluoride

Oxygen-independent killing by alveolar macrophages.

Topical Fluorides: A Discussion of Risks and Benefits

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