Cytotoxicity of Corylifoliae Fructus. I. Isolation of the Effective Compound and the Cytotoxicity

Bioactive Constituents from Chinese Natural Medicines. XX. Inhibitors of Antigen-Induced Degranulation in RBL-2H3 Cells from the Seeds of <i>Psoralea corylifolia</i>

“…The following experimental conditions were used for chromatography: ordinary-phase silica gel column chromatography, [6][7][8][9][10][11][12][36][37][38][39][40][41][42][43] or commercial samples. Table 1. 13 C-NMR (125 MHz, CDCl 3 ) d C : see Table 2.…”

“…The seeds of this plant have been used as a tonic, to treat uterine hemorrhage, and as a coronary vasodilatory agent in Chinese traditional medicine. [2][3][4][5] Previously, several meroterpenoid [6][7][8][9][10][11] and flavonoid [12][13][14][15][16][17][18][19] constituents were isolated from this natural medicine. In addition, biological studies to determine its antioxidative, 20) antiplatelet, 21) and DNA polymerase and topoisomerase II inhibitory activities 22) were also reported.…”

“…The EtOAc-acetate-soluble fraction inhibited antigen-induced degranulation in RBL-2H3 cells [inhibition (%): 39.3Ϯ2.5 (pϽ0.05) at 30 mg/ml], but the H 2 O-soluble fraction did not [inhibition (%): 6.3Ϯ4.4 at 30 mg/ml]. The EtOAc-soluble fraction was subjected to ordinary and reverse-phase silica gel column chromatography and finally HPLC to furnish 1 (0.011% from the natural medicine), 2 (0.009%), bakuchiol [6][7][8][9][10] (3, 8.19%), D 3 and 7-C; 10,14-H and 8-C; 8-H, 11,13-H and 9-C; 8-H and 10,14-C; 10,14-H and 12-C; 16-H and 15-C; 15-H 3 and 16-C), as shown in Fig. 1.…”

Bioactive Constituents from Chinese Natural Medicines. XXXIII. Inhibitors from the Seeds of Psoralea corylifolia on Production of Nitric Oxide in Lipopolysaccharide-Activated Macrophages

“…2) Previously, several meroterpenoid [3][4][5][6][7] and flavonoid [8][9][10][11][12][13][14][15] constituents were isolated from this natural medicine. In addition, a principal constituent, bakuchiol (1), and related compounds showed variety of bioactivities such as inhibition of monoamine transporters, 16) immunosuppressive effect, 17) caspase-3-depended apoptosis, 18) hepatoprotective effect, 19,20) antioxidant activity, [21][22][23] antimicrobial activity, 24) anti-inflammatory effect, 25) inhibition of DNA polymerase and topoisomerase II, 26) cytotoxic effect, 5,6,27) and anti-diabetic effects.…”

“…In addition, a principal constituent, bakuchiol (1), and related compounds showed variety of bioactivities such as inhibition of monoamine transporters, 16) immunosuppressive effect, 17) caspase-3-depended apoptosis, 18) hepatoprotective effect, 19,20) antioxidant activity, [21][22][23] antimicrobial activity, 24) anti-inflammatory effect, 25) inhibition of DNA polymerase and topoisomerase II, 26) cytotoxic effect, 5,6,27) and anti-diabetic effects. 28,29) In the course of our characterization studies on bioactive constituents from Chinese natural medicines, 1,[30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] we reported the inhibitors of antigen-induced degranulation in RBL-2H3 cells from this medicinal herb.…”