Cytotoxicity of alloying elements and experimental titanium alloys by WST-1 and agar overlay tests

Song, Yohan; Kim, Min-Kang; Park, Eun Jin; Song, Ho‐Jun; Anusavice, Kenneth J.; Park, Kyung Hee

doi:10.1016/j.dental.2014.05.012

Cited by 31 publications

(15 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Park et al and Song et al [107, 108] evaluated the biocompatibility of various alloying elements, as well as the binary Ti-alloys. The studies revealed that the cytocompatibility of pure metals ranked in the order of: Al > Ag > V > Mn > Cr > Zr > Nb > Mo > cp-Ti (Fig.…”

Section: Biological Compatibility Of Binary Ti-alloysmentioning

confidence: 99%

See 1 more Smart Citation

Binary titanium alloys as dental implant materials—a review

Liu

Chen

Tsoi

et al. 2017

Regenerative Biomaterials

202

View full text Add to dashboard Cite

Titanium (Ti) has been used for long in dentistry and medicine for implant purpose. During the years, not only the commercially pure Ti but also some alloys such as binary and tertiary Ti alloys were used. The aim of this review is to describe and compare the current literature on binary Ti alloys, including Ti–Zr, Ti–In, Ti–Ag, Ti–Cu, Ti–Au, Ti–Pd, Ti–Nb, Ti–Mn, Ti–Mo, Ti–Cr, Ti–Co, Ti–Sn, Ti–Ge and Ti–Ga, in particular to mechanical, chemical and biological parameters related to implant application. Literature was searched using the PubMed and Web of Science databases, as well as google without limiting the year, but with principle key terms such as ‘ Ti alloy’, ‘binary Ti ’, ‘Ti-X’ (with X is the alloy element), ‘dental implant’ and ‘medical implant’. Only laboratory studies that intentionally for implant or biomedical applications were included. According to available literatures, we might conclude that most of the binary Ti alloys with alloying <20% elements of Zr, In, Ag, Cu, Au, Pd, Nb, Mn, Cr, Mo, Sn and Co have high potential as implant materials, due to good mechanical performance without compromising the biocompatibility and biological behaviour compare to cp-Ti.

show abstract

Section: Biological Compatibility Of Binary Ti-alloysmentioning

confidence: 99%

“…(a) alloy elements: Al, Ag, V, Mn, Cr, Zr, Nb, Mo adapted from [107]; (b) alloy elements: Cu, in, Ag, Cr, Sn, Au, Pd, Pt, adapted from [108]…”

Section: Biological Compatibility Of Binary Ti-alloysmentioning

confidence: 99%

Binary titanium alloys as dental implant materials—a review

Liu

Chen

Tsoi

et al. 2017

Regenerative Biomaterials

202

View full text Add to dashboard Cite

show abstract

“…Among them, Co–Cr alloys exhibit excellent wear and corrosion resistance together with good mechanical stability when compared with titanium alloys and stainless steels (Chiba, Kumagai, Nomura, & Miyakawa, ; Saldivar‐Garcia & Lopez, ; Tkachenko et al, ). However, there has been some concern in regard to the osseointegration properties in terms of release of metal ions from Co–Cr alloys (Assad et al, ; Hornez, Lefevre, Joly, & Hildebrand, ; Puleo & Huh, ; Schrock et al, ; Song et al, ; Tsaousi, Jones, & Case, ). The release of metal ions may result in allergy and inflammation impacting the survival of the implant.…”

Section: Introductionmentioning

confidence: 99%

Favorable modulation of osteoblast cellular activity on Zr‐modified Co–Cr–Mo alloy: The significant impact of zirconium on cell–substrate interactions

et al. 2019

View full text Add to dashboard Cite

Cobalt‐chromium‐molybdenum alloys exhibit good mechanical properties (yield strength: ~530 MPa, ultimate tensile strength: ~1114 MPa, elongation‐to‐failure: ~47.3%, and modulus: ~227 GPa) and corrosion resistance. In recent years, from the perspective of osseointegration, they are considered to be lower in rank in comparison to the widely used titanium alloys. We elucidate here the significant and favorable modulation of cellular activity of Zr‐modified Co–Cr–Mo alloys. The average grain size of Co–Cr–Mo alloy samples with and without Zr was 104 ± 27 and ~53 ± 11 μm, respectively. The determining role of small addition of Zr (0.04 wt. %) to the Co–Cr–Mo alloys in favorable modulation of cellular activity was accomplished by combining cellular biology and materials science and engineering. Experiments on the influence of Zr addition to Co–Cr–Mo alloys clearly demonstrated that the cell adhesion, spread and cell–substrate interactions were enhanced in the presence of Zr. The spread/growth rate of cells was ~120% on the Co–Cr–Mo alloy and 190% per day on the Co–Cr–Mo–Zr alloy. While the % area covered by the cells increased from ~5.1 to ~33.6% on Co–Cr–Mo alloy and ~19.2 to ~47.8% on Co–Cr–Mo–Zr alloy after 2 and 24 hr of incubation. Similarly, the cell density increased from ~1354 to ~3424 cells/cm2 on Co–Cr–Mo alloy and ~3583 to ~7804 cells/cm2 on Co–Cr–Mo–Zr alloy after 2 and 24 hr of incubation. Additionally, stronger vinculin focal adhesion contact and signals associated with actin stress fibers together with extracellular matrix protein, fibronectin, were noted.

show abstract

“…Co and Cr ions have been demonstrated to produce highly cytotoxic effects, considerably higher than Ti ions . As observed in vitro , Co ions also reduce the osteogenic differentiation of mesenchymal stem cells and reduce concentration of integrin‐binding sialoprotein and osteocalcin, all of which may negatively influence osteogenesis .…”

Section: Introductionmentioning

confidence: 99%

“…8 Co and Cr ions have been demonstrated to produce highly cytotoxic effects, 9,10 considerably higher than Ti ions. [11][12][13] As observed in vitro, Co ions also reduce the osteogenic differentiation of mesenchymal stem cells and reduce concentration of integrin-binding sialoprotein and osteocalcin, all of which may negatively influence osteogenesis. 14 In contrast, Co ions are also known to mimic hypoxia through stabilizing the hypoxia inducible factor-1a, and have been incorporated into bioactive glasses to increase angiogenesis and vascular endothelial growth factor expression.…”

Section: Introductionmentioning

confidence: 99%

Osseointegration of 3D printed microalloyed CoCr implants—Addition of 0.04% Zr to CoCr does not alter bone material properties

Shah

Jergéus

Chiba

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Electron beam melting (EBM) is a three-dimensional (3D) printing technique for the production of metal structures where complex geometries with interconnected porosities can be built. Incorporation of as little as 0.04% Zr into the CoCr alloy can significantly improve the biomechanical anchorage of constructs fabricated by EBM. Here we investigate bone material properties, including microstructure and composition, adjacent to 3D printed CoCr implants with and without addition of 0.04% Zr, after 8 weeks of healing in the rabbit femur. In low amounts, zirconium addition does not alter the microstructure and extracellular matrix composition of bone formed adjacent to the surface of EBM manufactured implants. Bone ingrowth into surface irregularities of 3D printed CoCr and CoCr + Zr implants is seen. Extensive remodeling is also evident. Osteocytes attach directly on to the implant surface. The interfacial tissue at CoCr and CoCr + Zr has similar mineral crystallinity, apatite-to-collagen ratio, carbonate-to-phosphate ratio, Ca/P ratio, bone-implant contact, percentage porosity, and osteocyte density (N.Ot/B.Ar). Compared to the native bone, the mineral crystallinity of the interfacial tissue was lower while N.Ot/B.Ar was higher for both CoCr and CoCr + Zr. Overall, the results indicate that bone tissue adjacent to CoCr and CoCr + Zr implants is highly mature and exhibits comparable healing kinetics. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1655-1663, 2018.

show abstract

Cytotoxicity of alloying elements and experimental titanium alloys by WST-1 and agar overlay tests

Cited by 31 publications

References 54 publications

Binary titanium alloys as dental implant materials—a review

Binary titanium alloys as dental implant materials—a review

Favorable modulation of osteoblast cellular activity on Zr‐modified Co–Cr–Mo alloy: The significant impact of zirconium on cell–substrate interactions

Osseointegration of 3D printed microalloyed CoCr implants—Addition of 0.04% Zr to CoCr does not alter bone material properties

Contact Info

Product

Resources

About