Cytotoxicity Mediated by the Fas Ligand (FasL)-activated Apoptotic Pathway in Stem Cells

Mesenchymal stem cells control alloreactive CD8+CD28− T cells

“…− T cells, despite their ability to express Fas ligand (FasL) or to initiate the programmed death (PD)-1/PD-ligand 1 (PD-L1) pathway [57,58]. These observations indicate that MSC solely have an anti-proliferative effect on CD8 +…”

Gene Therapy and Stem Cell Therapy to Improve the Outcome of Human Islet Transplantation

“…Other literatures showed positive expression of FasL on the surface of BMSCs and hypothesized that BMSCs promoted the apoptosis of T cells and subsequent immune tolerance [58,146]. However, we did not detect FasL expression on the surface of BMSCs maintained in our lab (Figure 6-1).…”

“…Generally, the lack of co-stimulatory factors (CD80 and CD86) led to an immature antigen presenting to the Th cells and subsequent immune tolerance [151]. Despite that some groups showed that BMSCs expressed FasL and promoted the apoptosis of T cells through direct contact [146,152], our BMSCs were negative for FasL (Figure 6-2), which was supported by the results from other groups [55,153] and confirmed by the MLR that BMSCs did not promote the apoptosis of T cells (data not shown). The expression of HLA class II on the surface of BMSCs is still unclear.…”

“…We also tested CD14, CD80 and CD86 which are important co-stimulatory factors. Besides, FasL was also tested because they might participate in the apoptosis of T cells [146]. The BMSCs maintained in our lab were positive for HLA class I and negative for HLA-DR, FasL, CD14, CD80 and CD86 (Figure 6-2).…”

Biphasic effect of EGb761 on simulated ischemia-induced rat BMSC survival in vitro and in vivo

“…Previous data indicate that Fas and FasL are co-expressed on primary BMSCs (Mazar et al, 2009). FasL interacts with its receptor, Fas (CD95/APO-1), and triggers a cascade of subcellular events involving in caspase-8/caspase-3 signaling pathways and resulting in apoptotic cell death.…”