Cytotoxicity evaluation of large cyanobacterial strain set using selected human and murine in vitro cell models

Hrouzek, Pavel; Kapuścik, Aleksandra; Vacek, Jan; Voráčová, Kateřina; Paichlová, Jindřiška; Kosina, Pavel; Voloshko, Ludmila N.; Ventura, Stefano; Kopeckỳ, J.

doi:10.1016/j.ecoenv.2015.10.020

Cited by 19 publications

(8 citation statements)

References 44 publications

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“…Extracts from the cyanobacteria of marine, freshwater, or terrestrial origin, are commonly tested for their cytotoxicity by MTT test [57][58][59][60]. Notably, as in previous similar cytotoxicity screenings, the interaction between cyanobacteria extracts and cell cultures may induce rather different responses depending on cyanobacterial extract and the cell type tested [4,61,62].…”

Section: Discussionmentioning

confidence: 99%

The Marine Sponge Petrosia ficiformis Harbors Different Cyanobacteria Strains with Potential Biotechnological Application

Pagliara

Barca

Verri

et al. 2020

JMSE

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Marine cyanobacteria are a source of bioactive natural compounds, with a wide range of biotechnological applications. However, information on sponge-associated cyanobacteria are relatively scarce to date. In this paper, we carried out the morphological and molecular characterization of eight cyanobacterial strains, previously isolated from the Mediterranean sponge Petrosia ficiformis, and evaluated their biological activities on epithelial- and neuron-like cultured cells of human and murine origin. The new analysis allowed maintaining the assignment of three strains (Cyanobium sp., Leptolyngbya ectocarpi, and Synechococcus sp.), while two strains previously identified as Synechococcus sp. and Leptolyngbya sp. were assigned to Pseudanabaena spp. One strain, i.e., ITAC104, and the ITAC101 strain corresponding to Halomicronema metazoicum, shared extremely high sequence identity, practically representing two clones of the same species. Finally, for only one strain, i.e., ITAC105, assignment to a specific genus was not possible. Concerning bioactivity analyses, incubation of cyanobacterial aqueous cell supernatants induced variable responses in cultured cells, depending on cell type, with some of them showing toxic activity on human epithelial-like cells and no toxic effects on human and rat neuron-like cells. Future investigations will allow to better define the bioactive properties of these cyanobacteria strains and to understand if they can be useful for (a) therapeutic purpose(s).

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Section: Discussionmentioning

confidence: 99%

The Marine Sponge Petrosia ficiformis Harbors Different Cyanobacteria Strains with Potential Biotechnological Application

Pagliara

Barca

Verri

et al. 2020

JMSE

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“…In similar studies, marine Synechocystis and Leptolyngbya strains were found to induce cytotoxic effects in the hepatocellular carcinoma HepG2 cell line [20]. Further, Nostoc and Tolypothrix strains from soil habitats were also tested against the HepG2 cell line and 90% of crude cyanobacterial extracts were found to induce cytotoxic effects [67]. In all the above-mentioned cases, the compounds responsible for cytotoxicity, as well as the mechanisms of action, are not known.…”

Section: Discussionmentioning

confidence: 99%

Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece

Gkelis

Panou

Konstantinou

et al. 2019

Toxins

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Cyanobacteria are a diverse group of photosynthetic Gram-negative bacteria that produce an array of secondary compounds with selective bioactivity against a broad spectrum of organisms and cell lines. In this study, 29 strains isolated from freshwaters in Greece were classified using a polyphasic approach and assigned to Chroococcales, Synechococcales, and Nostocales, representing 11 genera and 17 taxa. There were good agreements between 16S ribosomal RNA (rRNA)–cpcBA–internal genetic spacer (IGS) characterization and morphological features, except for the Jaaginema–Limnothrix group which appears intermixed and needs further elucidation. Methanol extracts of the strains were analyzed for cyanotoxin production and tested against pathogenic bacteria species and several cancer cell lines. We report for the first time a Nostoc oryzae strain isolated from rice fields capable of producing microcystins (MCs) and a Chlorogloeopsis fritschii strain isolated from the plankton of a lake, suggesting that this species may also occur in freshwater temperate habitats. Strains with very high or identical 16S rRNA gene sequences displayed different antibacterial and cytotoxic activities. Extracts from Synechococcus cf. nidulans showed the most potent antibacterial activity against Staphylococcus aureus, whereas Jaaginema sp. strains exhibited potent cytotoxic activities against human colorectal adenocarcinoma and hepatocellular carcinoma cells. Jaaginema Thessaloniki Aristotle University Microalgae and Cyanobacteria (TAU-MAC) 0110 and 0210 strains caused pronounced changes in the actin network and triggered the formation of numerous lipid droplets in hepatocellular carcinoma and green monkey kidney cells, suggesting oxidative stress and/or mitochondrial damage leading to apoptosis.

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“…A problemática com as florações algais está relacionada à presença de cianobactérias potencialmente tóxicas, com efeitos negativos diretos à qualidade da água e à gestão hídrica (DROBAC et al, 2016;HROUZEK et al, 2016). As cianotoxinas apresentam grande quimiodiversidade (Quadro 1) e a sua toxicidade pode ser facilmente identificada e quantificada, medindo as respostas biológicas adequadas em organismos no ambiente ou expondo organismos em laboratório (ensaios ecotoxicológicos) (MARTINEZ-HAROA et al, 2015).…”

Section: Impactos Das Cianotoxinas à Saúde Humana E Ambientalunclassified

“…Exemplos destas pesquisas são bem recentes e geram certas controvérsias entre pesquisadores. Trabalho conduzido por Hrouzek et al (2016) utilizou células humanas em estudo sobre toxicidade de cianotoxinas de diferentes cianobactérias (Nostoc, Desmonostoc, Leptolyngbya, Phormidium, Cylindrospermum, Anabaena, Tolypothrix, Wollea, Synechocystis, Oscillatoria, Calothrix, Fisherella). A pesquisa mostrou um bom desempenho para testes utilizando modelos celulares in vitro, podendo estes serem utilizados como base para o acompanhamento da ação toxicológica de amostras de cianotoxinas em células do fígado e neoplásicas (cancerígenas).…”

Section: Ensaios Ecotoxicológicos Com Cianotoxinasunclassified

CIANOBACTÉRIAS E CIANOTOXINAS EM AMBIENTES AQUÁTICOS: uma breve revisão sobre os principais métodos detecção, remoção e impactos sobre a saúde humana = CYANBACTERIA AND CYANOTOXINS IN AQUATIC ENVIRONMENTS: a short review on the main methods of detection, removal and impacts on human health

Rodrigues

De-Carli

Vicentin

et al. 2021

Bol. Lab. Hidrobiol.

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Muitos corpos hídricos têm sido degradados devido à interferência humana. A poluição causada pelas atividades antrópicas vem comprometendo a qualidade da água e promovendo eventos de eutrofização, afetando toda estrutura trófica do ambiente, podendo assim acarretar na proliferação de cianobactérias tóxicas. Nesse sentido, o conhecimento sobre esses organismos é de grande importância, principalmente em corpos hídricos com usos múltiplos. Esta pesquisa representa uma revisão de trabalhos publicados envolvendo qualidade ambiental e ecolotoxicologia aquática, tendo como foco estudos relacionados às cianobactérias e cianotoxinas. Para o trabalho foram analisados livros textos e artigos publicados entre 2010 e 2020 em periódicos nacionais e internacionais. Na literatura publicada nos últimos anos são apresentadas as principais metodologias envolvidas na detecção e remoção de cianotoxinas, além de bioensaios ecotoxicológicos. Fica evidente a importância do tratamento de efluentes e controle das fontes de poluição difusas e pontuais no ecossistema promovendo a melhoria da qualidade da água e controle de cianobactérias.ABSTRACTCyanobacteria play an important role among producers in aquatic ecosystems, however when associated with eutrophication they become an ecological and public health problem. The problem involving cyanobacteria is the blooming of potentially toxic strains. The present work aims to present and discuss the problems related to the proliferation of cyanobacteria in limnological environments, and its consequences for dynamics in the environment and effects on environmental health. In this sense, knowledge about these organisms is of great importance, especially in water bodies with multiple uses. This research represents a review of published works involving environmental quality and aquatic ecotoxicology, focusing on studies related to cyanobacteria and cyanotoxins. For the present work, textbooks and articles published between 2010 and 2020 in national and internationaljournals were analyzed. In the literature published in recent years, the main methodologies involved in the detection and removal of cyanotoxins are presented, in addition to ecotoxicological bioassays. The study of cyanobacteria and their metabolites comprises an area of interest that reaches a greater dimension when impacts on the quality of water supply are evident, thus the importance of treating effluents and controlling pollution sources in the ecosystem is evident, promoting improvement and maintenance the quality of the water bodies.

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Cytotoxicity evaluation of large cyanobacterial strain set using selected human and murine in vitro cell models

Cited by 19 publications

References 44 publications

The Marine Sponge Petrosia ficiformis Harbors Different Cyanobacteria Strains with Potential Biotechnological Application

The Marine Sponge Petrosia ficiformis Harbors Different Cyanobacteria Strains with Potential Biotechnological Application

Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece

CIANOBACTÉRIAS E CIANOTOXINAS EM AMBIENTES AQUÁTICOS: uma breve revisão sobre os principais métodos detecção, remoção e impactos sobre a saúde humana = CYANBACTERIA AND CYANOTOXINS IN AQUATIC ENVIRONMENTS: a short review on the main methods of detection, removal and impacts on human health

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