Cytotoxicity Effect of the Pericarp Extracts of Garcinia forbesii King on MCF-7 Breast Cancer and HepG2 Liver Cancer Cell Lines

Abstract

No previous publications of study results regarding anticancer activity of the pericarp of G. forbesii King have been found. Therefore, in this study we assessed the cytotoxic effects of n-hexane, DCM, ethyl acetate, ABSTRACT Background: The species from the genus Garcinia has long been used as traditional medicine for cancer treatment. Objective: To analyze the phytochemical contents and assess the cytotoxic effects of pericarp extracts of Garcinia forbesii King against MCF-7 breast cancer cells and HepG2 liver cancer cells. Materials and Methods: The phytochemical contents were analyzed using the thin-layer chromatography and the cytotoxic activity was assessed using the MTT assay method. Results: Phytochemical screening showed the presence of alkaloids, flavonoids, terpenoids and polyphenols. The cytotoxic activities of n-hexane, DCM and ethyl acetate extracts on MCF-7 cells were shown with IC 50 103.605±2.3410 μg/ mL, 397.609±28.0534 μg/mL and 1,518.301±68.6379 μg/mL respectively, while the IC 50 on HepG2 cells were 79.798±1.2261 μg/mL, 83.230±4.2557 μg/mL and 671.875±94.3338 μg/mL respectively. Conclusion: The n-hexane, DCM and ethyl acetate extracts from pericarps of G. forbesii King have cytotoxic activities against MCF-7 and HepG2 cancer cells, therefore, it has the potential to be developed as an anticancer.

Correlation of Her-2 Expression with Clinicopathological Characteristics in Invasive Ductal Breast Cancer Patients

“…This study aims to investigate the correlation between Her-2 expression and clinicopathological characteristics in patients with invasive ductal breast carcinoma. Analysis of clinicopathological characteristics and expression of ER, PR, and Her-2 immunostaining are very useful tools for diagnosing this cancer, important for management protocol therapy and prognosis of the patient (10)(11)(12). This study aims to analyze the correlation of Her-2 expression with clinicopathological characteristics in patients with invasive ductal breast cancer.…”