Cytotoxicity Effect of Constituents of Pinus taiwanensis Hayata Twigs on B16-F10 Melanoma Cells

Abstract

Pinus taiwanensis Hayata (Pinaceae) is an endemic plant in Taiwan. According to the Chinese Materia Medica Grand Dictionary, the Pinus species is mainly used to relieve pain, and eliminate pus and toxicity. In this study, nineteen compounds were isolated from the ethyl acetate layer of the ethanolic extract of P. taiwanensis Hayata twigs using bioassay-guided fractionation, and their anti-melanoma effects were investigated through a B16-F10 mouse melanoma cell model. The structures of the purified compounds were identified by 2D-NMR, MS, and IR, including 1 triterpenoid, 9 diterpenoids, 2 lignans, 4 phenolics, 1 phenylpropanoid, 1 flavonoid, and 1 steroid. Among them, compound 3 was found to be a new diterpene. Some of the compounds (2, 5, 6, 17, 18) showed moderate cytotoxicity effects. On the other hand, the anti-melanoma effect was no better than that from the original ethyl acetate layer. We presumed it resulted from the synergistic effect, although further experimentation needs to be performed.

Anti-Aging Constituents from Pinus morrisonicola Leaves

“…In our study, we revealed flavonoids with sugar moieties and coumaroyl group or feruloyl group, besides the commonly reported hydroxy group-containing flavonoids in P. morrisonicola . Additionally, our findings demonstrated the presence of diterpenes, which is consistent with our previous finding on Pinus taiwanensis Hayata [ 31 ], while previous studies predominantly reported monoterpenes in Pinus species.…”

Principle active metabolites of Pinus morrisonicolaHayata synergistically inhibit cell proliferation and autophagy to elevate apoptosis in hepatocellular carcinoma cells

“…Further studies have shown that TW oil facilitates cell cycle arrest in HepG2 cells 23 . Chrysin is a biologically active dietary flavonoid in many plants and has been known to be the most active bioactive component in TW extract and has been known to induce apoptosis and suppress autophagic clearance 16,24,25 . Most of the studies conducted in cancer cells show that chrysin induces apoptosis and inhibits cancer proliferation.…”

“…induce apoptosis and suppress autophagic clearance. 16,24,25 Most of the studies conducted in cancer cells show that chrysin induces apoptosis and inhibits cancer proliferation. In lymphoblastoid B-cell lines, Chrysin activates the proapoptotic protein Bax and reduces the levels of antiapoptotic protein Bcl-2 and triggers mitochondrial permeability and elevates the release of cytochrome c into cytosol, and induces the activation of caspase-3 and subsequent apoptosis.…”