Cytotoxicity Comparison of Mineral Trioxide Aggregates and EndoSequence Bioceramic Root Repair Materials

Damas, Beth Ann; Wheater, Michelle; Bringas, Josef S.; Hoen, Michel

doi:10.1016/j.joen.2010.11.027

Cited by 162 publications

(168 citation statements)

References 19 publications

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“…The complete loss of viability in the presence of GIC corroborates previous studies (16), highlighting the need for the apposition of another protective material under GICs in deeper cavities. Acid release from GICs during setting represents a possible source of injury to the pulp cells following tubular diffusion and emphasizing the need for a barrier to such diffusion (16). Despite the presence of GIC in the experimental hybrid material, excellent cell viability (93%) was observed, which may reflect incorporation of the resin component (Bis-EMA 30), with its lower diffusion characteristics (10).…”

Section: Discussionsupporting

confidence: 88%

“…The pH values for MTA of around 12.0 have been reported in other studies (18,19). The complete loss of viability in the presence of GIC corroborates previous studies (16), highlighting the need for the apposition of another protective material under GICs in deeper cavities. Acid release from GICs during setting represents a possible source of injury to the pulp cells following tubular diffusion and emphasizing the need for a barrier to such diffusion (16).…”

Section: Discussionsupporting

confidence: 88%

“…The relatively poor viability seen with MTA compared with the control (49%) is suggestive of at least an initial toxic effect of MTA. While some of the previously published studies have reported higher cell viability in the presence of MTA (16,17), the data obtained during the first 24 h post-setting perhaps reflected the high pH generated by the material during this period (11). The pH values for MTA of around 12.0 have been reported in other studies (18,19).…”

Section: Discussionmentioning

confidence: 67%

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Novel experimental cements for use on the dentin-pulp complex

et al. 2012

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This aim of this study was to evaluate the physicochemical and biological properties of novel experimental cements (Hybrid, Paste and Resin) based on synergistic combinations of existing materials, including pH, diametral tensile strength (DTS) and cytotoxicity comparing them with mineral trioxide aggregate (MTA -Angelus ® ) and a glass ionomer cement (GIC) developed at our laboratory. For the physicochemical and biological tests, specimens with standard dimensions were produced. pH measurements were performed with digital pH meter at the following time intervals: 3, 24, 48 and 72 h. For the DTS test, cylindrical specimens were subjected to compressive load until fracture. The MTT assay was performed for cytotoxicity evaluation. Data were analyzed by ANOVA and Tukey's test (α=0.05). Paste group showed pH values similar to MTA, and Hybrid group presented pH values similar to GIC (p>0.05). The tested materials showed pH values ranging from alkaline to near neutrality at the evaluated times. MTA and GIC showed similar DTS values. The lowest and highest DTS values were seen in the Paste and Resin groups, respectively (p<0.05). Cell viability for MTA and experimental Hybrid, Paste and Resin groups was 49%, 93%, 90% and 86%, respectively, when compared with the control group. The photo-cured experimental resin cement showed similar or superior performance compared with the current commercial or other tested experimental materials.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 67%

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Novel experimental cements for use on the dentin-pulp complex

et al. 2012

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“…Part 5 of the biological evaluation of medical devices in ISO 10993-5 is as follows: Regulations of cytotoxicity in vitro, countries have to make the relevant provisions of the corresponding cytotoxicity tests according to their actual situation (13). With the continuous development of cytotoxicity tests, methods, such as detection of cell damage by morphological changes, determination of cell damage, measuring cell growth and metabolic properties, have appeared and have gradually been developed from qualitative evaluation to quantitative (14)(15)(16)(17). However, the correlation remains to be further studied, as well as the evaluation of the correlation results of these methods with other biological evaluation.…”

Section: Introductionmentioning

confidence: 99%

Study of the in vitro cytotoxicity testing of medical devices

Li¹,

Zhou²,

Xu³

2015

Biomedical Reports

348

224

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Abstract. The cytotoxicity test is one of the biological evaluation and screening tests that use tissue cells in vitro to observe the cell growth, reproduction and morphological effects by medical devices. Cytotoxicity is preferred as a pilot project test and an important indicator for toxicity evaluation of medical devices as it is simple, fast, has a high sensitivity and can save animals from toxicity. Three types of cytotoxicity test are stated in the International Organization for Standardization 109993-5: Extract, direct contact and indirect contact tests. The xCELLigence real-time cell analysis system shows a significant potential in regards to cytotoxicity in recent years. The present review provides a brief insight into the in vitro cytotoxicity testing of medical devices.

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“…In pilot studies, it was observed that this material started to set only when completely covered with water 29) . A recent study reported that EndoSequence Root Repair Material (marketed as iRoot BP in Canada; Brasseler USA, Savannah, GA, USA) incubated at 37°C in 100% humidity was unable to withstand a 500-g load until after a duration of 168 h (7 days) 30) . This has important clinical implications, especially where apical surgeries are involved, in that any unset material may be washed out by tissue fluids and blood in the surgical field and consequently lead to microleakage and treatment failure.…”

Section: Discussionmentioning

confidence: 99%

Effect of Er,Cr:YSGG laser irradiation on apical sealing ability of calcium silicatecontaining endodontic materials in root-end cavities

et al. 2014

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The aim of this research was to evaluate the apical sealing abilities of 60 root-end cavities filled with mineral trioxide aggregate (MTA) and iRoot BP cements after treated with either 17% EDTA solution or Er,Cr:YSGG laser irradiation. After the filling procedure, apical leakage quantity was measured at 4 weeks using a fluid filtration method. One root from each group was processed for scanning electron microscopy and energy dispersive X-ray spectroscopy analyses. Both EDTA/MTA and laser irradiation/MTA combinations showed significantly lower microleakage than EDTA/iRoot BP and laser irradiation/iRoot BP combinations (p<0.05). Between groups of the same filling material, there were no significant differences among specimens treated with EDTA or laser (p>0.05). Both MTA and iRoot-BP demonstrated tag-like structures within the dentinal tubules when used in conjunction with EDTA.

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Cytotoxicity Comparison of Mineral Trioxide Aggregates and EndoSequence Bioceramic Root Repair Materials

Cited by 162 publications

References 19 publications

Novel experimental cements for use on the dentin-pulp complex

Novel experimental cements for use on the dentin-pulp complex

Study of the in vitro cytotoxicity testing of medical devices

Effect of Er,Cr:YSGG laser irradiation on apical sealing ability of calcium silicatecontaining endodontic materials in root-end cavities

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