Cytotoxicity assays of new artificial tears containing 2-methacryloyloxyethyl phosphorylcholine polymer for ocular surface cells

Ayaki, Masahiko; Iwasawa, Atsuo; Niwano, Yoshimi

doi:10.1007/s10384-011-0073-8

Cited by 19 publications

(23 citation statements)

References 37 publications

(40 reference statements)

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“…The methods for cell culture, the cytotoxicity assays, and data evaluation have been described in detail elsewhere 2. Briefly, the following commercially available cell lines were used: SIRC (rabbit corneal epithelium; ATCC CCL-60; American Type Culture Collection [ATCC], Manassas, VA); BCE C/D-1b (bovine corneal epithelial cells; JCRB- 9129; Health Science Research Resource Bank, Osaka, Japan); RC-1 (rabbit corneal epithelium; JCRB-0246; Health Science Research Resource Bank); and Chang conjunctiva (human conjunctival cells; ATCC CCL-20.2; ATCC).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, patients’ ocular surface cells come in direct contact with eyedrop formulations. Thus, to reflect the actual situation, we have tried to improve cytotoxicity assays for ocular cells 2–6. In these studies, ocular surface cells came into direct contact with drug formulations in the absence of culture medium.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…However, in recent studies we have improved the CVS such that the CVS 50 is now defined as the number of measurements showing ≥50% cell viability and is expressed as a percentage over the range 0%–100% 2,3. Using this improved method, the total number of measurements is 72 (three concentrations, three exposure times, four cell lines, and two assays) 2,3. Because topically applied eyedrops can be affected by various factors, such as dilution by lacrimal fluid or concentration by moisture loss, and since they can come into contact with different cell types (eg, corneal epithelial and conjunctival cells) on the corneal surface for varying amounts of time, the cytotoxicity of eyedrops needs to be evaluated in a comprehensive manner.…”

Section: Introductionmentioning

confidence: 99%

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Comparative assessment of the cytotoxicity of six anti-inflammatory eyedrops in four cultured ocular surface cell lines, as determined by cell viability scores

Ayaki¹,

Iwasawa²,

Niwano

2012

OPTH

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PurposeAnti-inflammatory eyedrops are often used in the treatment of corneal epithelial disorders. In the present study, we evaluated the cytotoxicity of six anti-inflammatory eyedrops in four ocular surface cell lines.MethodsThe cytotoxicity of six commercially available anti-inflammatory ophthalmic solutions (ie, diclofenac, bromfenac, pranoprofen, betamethasone, and fluoromethorone) was assessed in three corneal cell lines and one conjunctival cell line. Cell viability was determined by the 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide and neutral red assays after exposing the cells to 10, 30, and 60 minutes of onefold, twofold, and tenfold dilutions of the drugs. Cytotoxicity was compared using the cell viability score (CVS), an integrated cytotoxic parameter that takes various factors into account, such as dilution by tear fluid or concentration by evaporation, drug exposure time, and ocular surface cell type.ResultsBased on the CVS scores, the order of the anti-inflammatory eyedrops tested from least to most cytotoxic, with the active ingredient %CVS50, and %CVS40/80 for each solution given in parentheses, was as follows: Rinderon® (betamethasone, 100%, 100%) >0.02% Flumethoron® (fluoromethorone, 68%, 22%) = 0.1% Flumethoron® (fluoromethorone, 76%, 22%) >Bronuck® (0.1% bromfenac, 53%, −8%) = Diclod® (0.1% diclofenac, 44%, −15%) = Niflan® (pranoprofen, 50%, −19%). Rinderon® exhibited the least toxicity of all the anti-inflammatory eyedrops tested. Eyedrops containing non-steroidal anti-inflammatory drugs exhibited greater cytotoxicity than those containing steroids with benzalkonium at comparable concentrations. Concentration was the most significant factor affecting cell viability.ConclusionThe cytotoxicity of the anti-inflammatory eyedrops evaluated in the present study depended on both the pharmaceutical components and preservatives. The CVS is a concise indicator of drug cytotoxicity.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative assessment of the cytotoxicity of six anti-inflammatory eyedrops in four cultured ocular surface cell lines, as determined by cell viability scores

Ayaki¹,

Iwasawa²,

Niwano

2012

OPTH

Self Cite

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“…Lipidure®, a copolymer of butyl methacrylate and 2-methacryloyloxyethyl phosphorylcholine, was chosen as the coating material; this material has been previously shown to reduce the adherence of fibroblasts on poly(ethylene telephthalate) in comparison with uncoated material [23]. In previous work, Mang et al and Ayaki et al [24, 25] showed using in vitro studies that Lipidure® materials provide good cytocompatibility. To determine the effectiveness of Lipidure®-coated E-shell 300 materials in preventing macrophage adhesion, we examined uncoated materials and coated materials (n=3) at 2 hours, 24 hours, and 48 hours (data not shown) after seeding.…”

mentioning

confidence: 99%

Multiplexed microneedle-based biosensor array for characterization of metabolic acidosis

Miller

Skoog

Edwards

et al. 2012

Talanta

111

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The development of a microneedle-based biosensor array for multiplexed in situ detection of exercise-induced metabolic acidosis, tumor microenvironment, and other variations in tissue chemistry is described. Simultaneous and selective amperometric detection of pH, glucose, and lactate over a range of physiologically-relevant concentrations in complex media is demonstrated. Furthermore, materials modified with a cell-resistant (Lipidure®) coating were shown to inhibit macrophage adhesion; no signs of coating delamination were noted over a 48-hour period.

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Tuning of Smart Multifunctional Polymer Coatings Made by Zwitterionic Phosphorylcholines

Münch

Adam

Fritzsche

et al. 2019

Adv Materials Inter

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In the last years, the generation of multifunctional coatings has been moved into the focus of interface modifications to expand the spectrum of material applications and to introduce new smart properties. Herein a promising multifunctional and universally usable coating with simultaneous antifouling, easy‐to‐clean, and anti‐fog functionality is presented based on smart polymer films consisting of copolymers with 2‐methacryloyloxyethyl phosphorylcholine (MPC), realizing the function of the film and photoreactive 4‐benzophenyl methacrylate (BPO), which is responsible for stability and crosslinking. The easy‐to‐clean effect is demonstrated qualitatively and quantitatively by oil droplet detachment experiments. The antifouling behavior against different germs is investigated by cell adhesion experiments. Furthermore the anti‐fog performance is shown by breathing on the surfaces. To study the influence of the different amounts of copolymerized BPO, the grafted films are characterized by atomic force microscopy (AFM), infrared spectroscopy (ATR‐FTIR), as well as contact angle measurements. In situ spectroscopic ellipsometry is performed to investigate the swelling behavior of the thin films as a function of the time of UV‐irradiation. It is found that a degree of swelling of 15 and a water contact angle of less than 12° are the key parameters necessary for the generation of multifunctional coatings.

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Cytotoxicity assays of new artificial tears containing 2-methacryloyloxyethyl phosphorylcholine polymer for ocular surface cells

Abstract: The MPC eye drops were tolerable to ocular surface cells, and comparable to single doses of clinically approved drugs containing sodium hyaluronate.

Cited by 19 publications

References 37 publications

Comparative assessment of the cytotoxicity of six anti-inflammatory eyedrops in four cultured ocular surface cell lines, as determined by cell viability scores

Comparative assessment of the cytotoxicity of six anti-inflammatory eyedrops in four cultured ocular surface cell lines, as determined by cell viability scores

Multiplexed microneedle-based biosensor array for characterization of metabolic acidosis

Tuning of Smart Multifunctional Polymer Coatings Made by Zwitterionic Phosphorylcholines

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