Cytotoxicity, apoptosis, and IL-8 gene expression induced by some foodborne pathogens in presence of Bacillus coagulans in HT-29 cells

Kawarizadeh, Amin; Pourmontaseri, Maryam; Farzaneh, Mina; Hossinzadeh, Saeid; Pourmontaseri, Zahra

doi:10.1016/j.micpath.2020.104685

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…The cell volume became smaller with irregular morphology. Other studies have also confirmed that the death of tumor cells caused by probiotics is caused by apoptosis rather than necrosis [ 23 , 26 , 27 ]. Our flow cytometry results further demonstrated that B. coagulans MZY531 induced the decrease of H22 hepatoma cells activity, which was caused by apoptosis.…”

Section: Discussionmentioning

confidence: 78%

Anticancer potential of Bacillus coagulans MZY531 on mouse H22 hepatocellular carcinoma cells via anti-proliferation and apoptosis induction

Zhao,

Yang,

Zhou

et al. 2023

BMC Complement Med Ther

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Bacillus coagulans have recently revealed its anticancer effects, but few investigations are available on their effects on liver cancer proliferation, and the precise mechanism to mark its impact on apoptosis-related signaling pathways has yet to be elucidated. The aim of this study was to evaluate the anti-proliferative effect of B. coagulans MZY531 and apoptosis induction in the mouse H22 hepatocellular carcinoma cell line. The anti-proliferative activity of B. coagulans MZY531 was evaluated by Cell Counting Kit-8 (CCK-8) assay, and cell apoptosis was revealed with Terminal Deoxynucleotidyl Transferase (TDT)-mediated dUTP Nick-End Labeling (TUNEL) staining and flow cytometric analysis. The expressions of apoptosis-related protein were determined by western blot analysis. The CCK-8 assay revealed that B. coagulans MZY531 inhibited the H22 cells proliferation in a concentration-dependent manner. TUNEL staining revealed an increased apoptosis rate in H22 cells following intervention with B. coagulans MZY531. Furthermore, flow cytometric analysis showed that B. coagulans MZY531 treatment (MOI = 50 and 100) significantly alleviated the H22 cells apoptosis compared with the control group. Western blot analysis found B. coagulans MZY531 significantly decreased level of phospho-PI3K (p-PI3K), phospho-AKT (p-AKT), and phospho-mTOR (p-mTOR) compared with the control group. Furthermore, H22 cells treatment with B. coagulans MZY531 enhanced the expression of caspase-3 and Bax and jeopardized the expression of Bcl-2. Taken together, apoptosis induction and cell proliferation inhibition via PI3K/AKT/mTOR and Bax/Bcl-2/Caspase-3 pathway are promising evidence to support B. coagulans MZY531 as a potential therapeutic agent for cancer.

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Section: Discussionmentioning

confidence: 78%

Anticancer potential of Bacillus coagulans MZY531 on mouse H22 hepatocellular carcinoma cells via anti-proliferation and apoptosis induction

Zhao,

Yang,

Zhou

et al. 2023

BMC Complement Med Ther

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“…Cytotoxicity test was evaluated by hemocytometer using the existing method 47 . Neuronal Schwann cell line RSC96 (ATCC CRL‐2765) was employed in this study.…”

Section: Methodsmentioning

confidence: 99%

“…Cytotoxicity test was evaluated by hemocytometer using the existing method. 47 Neuronal Schwann cell line RSC96 (ATCC CRL-2765) was employed in this study. The cell was grown in Dulbecco's Modification of Eagle's Medium (DMEM) supplemented with 10% foetal bovine serum (FBS) and 1% penicillin/streptomycin, then incubated at 37 C with 5% CO 2.…”

Section: Cytotoxicity Testmentioning

confidence: 99%

Utilising N‐glutaryl chitosan‐based film with butterfly pea flower anthocyanin as a freshness indicator of chicken breast

et al. 2023

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A novel indicator film was produced with N‐glutaryl chitosan (ChGA) as a matrix and butterfly pea flower anthocyanin (BPA) as a dye. The synthesis of ChGA was achieved by the reaction of chitosan (Ch) and glutaric anhydride (GA), which resulted in the structural alteration of Ch, with carboxylic groups being present in ChGA. The ChGA–BPA film demonstrated higher sensitivity to changes in pH, a more compact structure, and more hydrophilic properties compared with Ch‐BPA film. The ChGA–BPA film demonstrated antioxidant and antimicrobial activity, as well as an enhanced biodegradable property. Further characterisation showed ChGA–BPA film possessed ultraviolet visible (UV–Vis) light barrier properties and was non‐toxic to cells. Lastly, when tested as packaging, the films underwent a visible colour changes from blue to yellowish‐green as chicken breast was stored at various temperatures, which correlated with an increase in nitrogen compounds, indicating the potential of this film as a smart packaging solution to monitor the freshness of chicken breast.

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“…The simplest model used in Salmonella –host–microbiota interactions is confluent cell monolayers of IECs, such as IPEC-J2 ( Schierack et al, 2011 ), IEC-6, or IEC-8 ( Khailova et al, 2010 ; Liu et al, 2010 ) or cells of cancerous origin, such as HT-29 and Caco-2 ( Carey and Kostrzynska, 2013 ; Kawarizadeh et al, 2021 ). Cell lines of normal tissue origin are morphologically and functionally similar to primary cells, develop proper microvilli, tight junctions, and Toll-like receptors (TLRs).…”

Section: Cell Linesmentioning

confidence: 99%

The Game for Three: Salmonella–Host–Microbiota Interaction Models

Grzymajło

2022

Front. Microbiol.

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Colonization of the gastrointestinal (GI) tract by enteric pathogens occurs in a context strongly determined by host-specific gut microbiota, which can significantly affect the outcome of infection. The complex gameplay between the trillions of microbes that inhabit the GI tract, the host, and the infecting pathogen defines a specific triangle of interaction; therefore, a complete model of infection should consider all of these elements. Many different infection models have been developed to explain the complexity of these interactions. This review sheds light on current knowledge, along with the strengths and limitations of in vitro and in vivo models utilized in the study of Salmonella–host–microbiome interactions. These models range from the simplest experiment simulating environmental conditions using dedicated growth media through in vitro interaction with cell lines and 3-D organoid structure, and sophisticated “gut on a chip” systems, ending in various animal models. Finally, the challenges facing this field of research and the important future directions are outlined.

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Cytotoxicity, apoptosis, and IL-8 gene expression induced by some foodborne pathogens in presence of Bacillus coagulans in HT-29 cells

Cited by 7 publications

References 24 publications

Anticancer potential of Bacillus coagulans MZY531 on mouse H22 hepatocellular carcinoma cells via anti-proliferation and apoptosis induction

Anticancer potential of Bacillus coagulans MZY531 on mouse H22 hepatocellular carcinoma cells via anti-proliferation and apoptosis induction

Utilising N‐glutaryl chitosan‐based film with butterfly pea flower anthocyanin as a freshness indicator of chicken breast

The Game for Three: Salmonella–Host–Microbiota Interaction Models

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