Cytotoxicity, antioxidant, anti-inflammatory activity, and GC-MS analysis of Egyptian propolis

Ezzat, S. M.; Khattaby, A. M.; Abdelmageed, Shimaa; El-Aal, M. Abd

doi:10.1007/s00580-019-02971-6

Cited by 10 publications

(5 citation statements)

References 52 publications

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“…Moreover, the found IC50 values are in good agreement with many previous works (Pratami et al, 2017;da Silva et al, 2019;Touzani et al, 2018). Nevertheless, the antioxidant activities of different Lebanese propolis are lower than those of Egyptian propolis (Ezzat et al, 2019).…”

Section: Dpph Assaymentioning

confidence: 84%

Chemical Analysis and Antioxidant Activity of Four Propolis Samples Collected from Different Regions of Lebanon

Ali

Jaber

Dorra

et al. 2021

Journal of Apitherapy and Nature

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Propolis samples were collected from four different regions of Lebanon characterized by high biodiversity and high honey production. The samples were analyzed for their total phenolic contents (TPC), total flavonoid contents (TFC), chemical profiles, and antioxidant activity. The TPC was determined using Folin-Ciocalteu method while the TFC using the AlCl3 method. The antioxidant activity of propolis was examined by two different methods, namely free radical scavenging assay and reducing ability. The chemical profiles of the samples were assessed by thin-layer chromatography (TLC), UV-Vis, and gas chromatography/mass spectrometry GC-MS analysis. Total phenolic content ranged from 53.35 ± 7.09 to 148.27 ± 15.08 mg GAE/g, total flavonoid content ranged from 45.73 ± 2.8 to 134.5 ± 8.46 mg RUE/g. GC/MS analysis revealed the presence of 9-octadecene and tetradecene as major compounds that have been previously reported to demonstrate antioxidant activity. In addition, Berqayel propolis sample showed high content of phenolic compounds and high antioxidant activity and contained mainly hydrocarbons, while samples from Wadi Faara recorded poor chromatograms with the absence of most of the compounds present in Berqayel samples. The majority of propolis samples showed relatively interesting antioxidant activity, which was also correlated with TPC and TFC. Our results provide additional evidence on the importance of the chemical composition of propolis and its usefulness in the pharmaceutical industry.

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Section: Dpph Assaymentioning

confidence: 84%

Chemical Analysis and Antioxidant Activity of Four Propolis Samples Collected from Different Regions of Lebanon

Ali

Jaber

Dorra

et al. 2021

Journal of Apitherapy and Nature

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“…Of the 27 compounds identified by the aforementioned means, 12 of them are well-documented in Egyptian and worldwide propolis. These include d-glucopyranose [ 47 – 49 ] (10.6%); chrysin [ 46 – 48 , 50 – 59 ] (8.1%); sucrose [ 47 , 48 ] (5.8%); 1-[4-hydroxyphenyl]-3-[2,4-dihydroxyphenyl]-2-propen-1-one [ 57 ] (4.7%); genistein [ 53 , 54 , 56 – 58 , 60 ] (4%); myo-inositol [ 50 , 61 ] (2.5%); isoferulic acid [ 46 , 50 , 52 , 58 – 60 , 62 ] (1.8%); palmitic acid [ 46 – 48 , 50 – 52 , 57 , 58 , 60 , 62 – 65 ] (0.5%); d-mannitol [ 47 , 48 ] (0.4%); linoleic acid [ 46 , 50 , 52 , 58 ] (0.3%); d-gluconic acid [ 50 , 52 , 63 ] (0.2%); and gallic acid [ 52 , 58 , 66 ] (0.1%).…”

Section: Resultsmentioning

confidence: 99%

Synergistic effect of potential alpha-amylase inhibitors from Egyptian propolis with acarbose using in silico and in vitro combination analysis

Nada,

Metwally,

Asaad

et al. 2024

BMC Complement Med Ther

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Background Type 2 Diabetes mellitus (DM) is an affliction impacting the quality of life of millions of people worldwide. An approach used in the management of Type 2 DM involves the use of the carbohydrate-hydrolyzing enzyme inhibitor, acarbose. Although acarbose has long been the go-to drug in this key approach, it has become apparent that its side effects negatively impact patient adherence and subsequently, therapeutic outcomes. Similar to acarbose in its mechanism of action, bee propolis, a unique natural adhesive biomass consisting of biologically active metabolites, has been found to have antidiabetic potential through its inhibition of α-amylase. To minimize the need for ultimately novel agents while simultaneously aiming to decrease the side effects of acarbose and enhance its efficacy, combination drug therapy has become a promising pharmacotherapeutic strategy and a focal point of this study. Methods Computer-aided molecular docking and molecular dynamics (MD) simulations accompanied by in vitro testing were used to mine novel, pharmacologically active chemical entities from Egyptian propolis to combat Type 2 DM. Glide docking was utilized for a structure-based virtual screening of the largest in-house library of Egyptian propolis metabolites gathered from literature, in addition to GC–MS analysis of the propolis sample under investigation. Thereafter, combination analysis by means of fixed-ratio combinations of acarbose with propolis and the top chosen propolis-derived phytoligand was implemented. Results Aucubin, identified for the first time in propolis worldwide and kaempferol were the most promising virtual hits. Subsequent in vitro α-amylase inhibitory assay demonstrated the ability of these hits to significantly inhibit the enzyme in a dose-dependent manner with an IC50 of 2.37 ± 0.02 mM and 4.84 ± 0.14 mM, respectively. The binary combination of acarbose with each of propolis and kaempferol displayed maximal synergy at lower effect levels. Molecular docking and MD simulations revealed a cooperative binding mode between kaempferol and acarbose within the active site. Conclusion The suggested strategy seems imperative to ensure a steady supply of new therapeutic entities sourced from Egyptian propolis to regress the development of DM. Further pharmacological in vivo investigations are required to confirm the potent antidiabetic potential of the studied combination.

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“…Selective inhibition of COX-2 could alleviate the side effects associated with inhibiting the COX-1 isoform as antiinflammatory agent, and that was set as the initial goal for this study (Chirisa and Mukanganyama, 2016). Natural products, specifically medicinal herbs and drug discovery remained a successful combination for the inventorization of new therapeutical agents (Ezzat et al, 2019). site or it may be due to the release of serotonin and histamine (Saleem et al, 2017a).…”

Section: Discussionmentioning

confidence: 99%

Untitled

2021

Pak. J. Pharm. Sci

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Study has been premeditated to appraise the anticancer and anti-inflammatory activities of a native medicinal plant Saussurea hypoleuca Spreng root. Anticancer assays including MTT, Alamar Blue (AB), Neutral Red (NR) & LDH were employed on root methanolic extract (RME) and all fractions to calculate % age of cell viability and cell cytotoxicity. All fractions of plant root were tested for in vitro as well as in vivo anti-inflammatory assays by reported methods. GC-MS analysis of n-hexane: chloroform fractions in column chromatography has shown isopropyl myristate, hexadecanoic acid, 11-octadecenoic acid, Di-n-octyl phthalate, dioctyl ether, decanedioic acid, 1H-3a,7-Methanoazulene, 3,4-hexanedione and Tetracosapentaene. Percentage of cell viability in anticancer assays was significantly high in all fractions. However, whole results were momentous with ethyl acetate and aqueous fractions owning to excellent profile in evaluating cytotoxicity in each assay. COX-2 inhibition was calculated which was high in RME (68.69%), ethyl acetate (56.52%), aqueous (55.21%) and chloroform fraction (53.47%). Carrageenan and formalin models were developed on rats to investigate in vivo anti-inflammatory activity. RME (56.19%, 71.09%, 66.4%, 67.99%) and ethyl acetate (51.36%, 64.97%, 55.63% & 61.01%) produced significant % age inhibition in dose dependent manner at 200 and 400 mg/kg doses respectively. All above findings direct that plant root holds strong anticancer and antiinflammatory activities.

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Cytotoxicity, antioxidant, anti-inflammatory activity, and GC-MS analysis of Egyptian propolis

Cited by 10 publications

References 52 publications

Chemical Analysis and Antioxidant Activity of Four Propolis Samples Collected from Different Regions of Lebanon

Chemical Analysis and Antioxidant Activity of Four Propolis Samples Collected from Different Regions of Lebanon

Synergistic effect of potential alpha-amylase inhibitors from Egyptian propolis with acarbose using in silico and in vitro combination analysis

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