Cytotoxicity, Antibiotic Combination and Antiviral Activity of Papain Enzyme: <i>In vitro</i> study

Mohamed, Sara H.; Mohamed, Walaa S.; Shaheen, Mohamed N F; Elmahdy, Elmahdy M.; Mabrouk, Mona I.

doi:10.5958/2231-5659.2020.00002.8

Cited by 3 publications

(3 citation statements)

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“…Song et al (2020) observed that papain at 5.0 μg/mL resulted in 26.1, 21.6 and 50.9% reductions in the biofilm mass formed by S. aureus, C. jejuni NCTC 11168 and C. jejuni Y23-5, respectively. Accordingly, Mohamed et al (2018) demonstrated that 100 mg/mL of papain removed 59% of biofilms formed by Klebsiella pneumoniae, although no effects on planktonic cells were observed.…”

Section: Biofilm Formation and Structurementioning

confidence: 99%

“…L. monocytogenes has been reported in raw milk of cow, sheep and goat (Mansouri-Najand et al, 2015) as well as in readyto-eat dairy products such as cheese (Jakobsen et al, 2011) and ice cream (Chen et al, 2016). Other bacteria frequently found in milk and dairy products, with economic and health interest, as well as biofilm formation ability, include several species of Pseudomonas such as P. fluorescens (Meng et al, 2017), Klebsiela pnemoniae (Mohamed et al, 2018), Mycobacterium avium subsp. paratuberculosis (Mullan, 2019), Campylobacter jejuni (Song et al, 2020), S. enterica and E. coli O157:H7 (Ahmed and Shimamoto, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Enzyme-based approaches to control microbial biofilms in dairy processing environments: A review

Gonçalves

Lee

Khaneghah

et al. 2020

qas

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In this review, formation of biofilms and the available data on enzyme-based preparations to control microbial biofilms in dairy processing environments are presented. Mature biofilms, especially those formed by pathogenic bacteria, have increased resistance to biocides, hence stressing the importance of stringent hygienic procedures. Proteases, amylases, cellulases and DNAses are among the most recently studied enzymes that could be associated with the main biocides used in the dairy industry to increase the effect of removal of biofilm. However, addi-tional studies should be conducted to select the best cost-benefit combinations of selected enzymes and biocides to remove efficiently biofilms in dairy processing environments.

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Section: Biofilm Formation and Structurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enzyme-based approaches to control microbial biofilms in dairy processing environments: A review

Gonçalves

Lee

Khaneghah

et al. 2020

qas

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“…After a 24 h of treatment, the plate was examined via an Olympus (Tokyo, Japan) CKX41 inversion phase contrast tissue culture microscope equipped with an Optika (Las Vegas, NV, USA) Pro5 CCD camera, and the microscopic results were recorded. Cell morphological modifications, such as rounding or shrinking, granulation, and vacuolization in the cytoplasm, were all taken into consideration as evidence of cytotoxicity [70][71][72].…”

Section: Mtt Assaymentioning

confidence: 99%

A Study on the Antibacterial, Antispasmodic, Antipyretic, and Anti-Inflammatory Activity of ZnO Nanoparticles Using Leaf Extract from Jasminum sambac (L. Aiton)

Sugitha,

Venkatesan,

Latha

et al. 2024

Molecules

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The green synthesis of zinc oxide nanoparticles (ZnO NPs) using plants has grown in significance in recent years. ZnO NPs were synthesized in this work via a chemical precipitation method with Jasminum sambac (JS) leaf extract serving as a capping agent. These NPs were characterized using UV-vis spectroscopy, FT-IR, XRD, SEM, TEM, TGA, and DTA. The results from UV-vis and FT-IR confirmed the band gap energies (3.37 eV and 3.50 eV) and the presence of the following functional groups: CN, OH, C=O, and NH. A spherical structure and an average grain size of 26 nm were confirmed via XRD. The size and surface morphology of the ZnO NPs were confirmed through the use of SEM analysis. According to the TEM images, the ZnO NPs had an average mean size of 26 nm and were spherical in shape. The TGA curve indicated that the weight loss starts at 100 °C, rising to 900 °C, as a result of the evaporation of water molecules. An exothermic peak was seen during the DTA analysis at 480 °C. Effective antibacterial activity was found at 7.32 ± 0.44 mm in Gram-positive bacteria (S. aureus) and at 15.54 ± 0.031 mm in Gram-negative (E. coli) bacteria against the ZnO NPs. Antispasmodic activity: the 0.3 mL/mL sample solution demonstrated significant reductions in stimulant effects induced by histamine (at a concentration of 1 µg/mL) by (78.19%), acetylcholine (at a concentration of 1 µM) by (67.57%), and nicotine (at a concentration of 2 µg/mL) by (84.35%). The antipyretic activity was identified using the specific Shodhan vidhi method, and their anti-inflammatory properties were effectively evaluated with a denaturation test. A 0.3 mL/mL sample solution demonstrated significant reductions in stimulant effects induced by histamine (at a concentration of 1 µg/mL) by 78.19%, acetylcholine (at a concentration of 1 µM) by 67.57%, and nicotine (at a concentration of 2 µg/mL) by 84.35%. These results underscore the sample solution’s potential as an effective therapeutic agent, showcasing its notable antispasmodic activity. Among the administered doses, the 150 mg/kg sample dose exhibited the most potent antipyretic effects. The anti-inflammatory activity of the synthesized NPs showed a remarkable inhibition percentage of (97.14 ± 0.005) at higher concentrations (250 µg/mL). Furthermore, a cytotoxic effect was noted when the biologically synthesized ZnO NPs were introduced to treated cells.

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Detection of Antimicrobial, Antioxidant and Cytotoxicity Activities of Fusarium oxysporum F01 Isolated from Catharanthus roseus Collected in Vietnam

Tu¹,

Thuy²,

Vinh³

et al. 2021

J. Pure Appl. Microbiol.

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The ability of endophytic fungi to produce valuable bioactive compounds when surviving in medicinal herbs. Finding out endophytic fungi originated from Catharanthus roseus with antimicrobial, antioxidant and cytotoxicity activities is important for pharmaceutical development. The isolation was based on the morphology of fungi. Identification was performed by sequencing 18S rRNA that was compared with known genes using Blast search in the combination of phylogenic analysis by using Clustal W and PhyML in GenomeNet. For the antimicrobial test, the agar diffusion method was used. DPPH scavenging assay for the antioxidant activity determination by using spectrophotometry. The cytotoxicity test was carried out by Sulforhodamine B method. LC-MS was applied for predicting components. We isolated the Fusarium oxysporum F01 strain originated from Catharanthus roseus. The aqueous extracts showed inhibition against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 (15.00 ± 0.50 mm), Serratia marcescens ATCC 14756 (9.00 ± 0.87 mm), Vibrio parahaemolyticus ATCC 17802 (15.50 ± 0.87 mm), Escherichia coli ATCC 25922 (12.50 ± 0.50 mm). The antioxidant activity of the extract obtained from the supernatant was determined with an IC50 value of about 11 µg/mL. The extract also showed cytotoxicity effect on liver cancer cell line (HepG2) and breast cancer cell line (MCF-7) with the percentage of inhibition of 84.47 ± 3.18 and 94.69 ± 1.59, respectively. LC-MS was used to point out the presence of pratol, a melanogenesis agent. The study provided more interesting information about Fusarium oxysporum F01 isolated in Catharanthus roseus grown in Vietnam, contributing to pharmaceutical sources in the world.

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Cytotoxicity, Antibiotic Combination and Antiviral Activity of Papain Enzyme: In vitro study

Cited by 3 publications

References 0 publications

Enzyme-based approaches to control microbial biofilms in dairy processing environments: A review

Enzyme-based approaches to control microbial biofilms in dairy processing environments: A review

A Study on the Antibacterial, Antispasmodic, Antipyretic, and Anti-Inflammatory Activity of ZnO Nanoparticles Using Leaf Extract from Jasminum sambac (L. Aiton)

Detection of Antimicrobial, Antioxidant and Cytotoxicity Activities of Fusarium oxysporum F01 Isolated from Catharanthus roseus Collected in Vietnam

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